In:
Journal of Virology, American Society for Microbiology, Vol. 73, No. 9 ( 1999-09), p. 7108-7116
Abstract:
The ex vivo antiviral CD8 + repertoires of 34 human immunodeficiency virus (HIV)-seropositive patients with various CD4 + T-cell counts and virus loads were analyzed by gamma interferon enzyme-linked immunospot assay, using peptides derived from HIV type 1 and Epstein-Barr virus (EBV). Most patients recognized many HIV peptides, with markedly high frequencies, in association with all the HLA class I molecules tested. We found no correlation between the intensity of anti-HIV CD8 + responses and the CD4 + counts or virus load. In contrast, the polyclonality of anti-HIV CD8 + responses was positively correlated with the CD4 + counts. The anti-EBV responses were significantly less intense than the anti-HIV responses and were positively correlated with the CD4 + counts. Longitudinal follow-up of several patients revealed the remarkable stability of the anti-HIV and anti-EBV CD8 + responses in two patients with stable CD4 + counts, while both antiviral responses decreased in two patients with obvious progression toward disease. Last, highly active antiretroviral therapy induced marked decreases in the number of anti-HIV CD8 + T cells, while the anti-EBV responses increased. These findings emphasize the magnitude of the ex vivo HIV-specific CD8 + responses at all stages of HIV infection and suggest that the CD8 + hyperlymphocytosis commonly observed in HIV infection is driven mainly by virus replication, through intense, continuous activation of HIV-specific CD8 + T cells until ultimate progression toward disease. Nevertheless, highly polyclonal anti-HIV CD8 + responses may be associated with a better clinical status. Our data also suggest that a decrease of anti-EBV CD8 + responses may occur with depletion of CD4 + T cells, but this could be restored by highly active antiretroviral treatment.
Type of Medium:
Online Resource
ISSN:
0022-538X
,
1098-5514
DOI:
10.1128/JVI.73.9.7108-7116.1999
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1999
detail.hit.zdb_id:
1495529-5
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