In:
FEBS Letters, Wiley, Vol. 440, No. 1-2 ( 1998-11-27), p. 19-24
Abstract:
Here we report that photoactivated hypericin can induce either apoptosis or necrosis in HeLa cells. Under apoptotic conditions the cleavage of poly(ADP‐ribose) polymerase (PARP) into the 85‐kDa product is blocked by the caspase inhibitors benzyloxycarbonyl‐Val‐Ala‐Asp‐fluoromethylketone (z‐VAD‐fmk) and benzyloxycarbonyl‐Asp‐Glu‐Val‐Asp‐fluoromethylketone (z‐DEVD‐fmk). Both inhibitors protect cells from apoptosis but cannot prevent hypericin‐induced necrosis. Conversely, HeLa cells overexpressing the viral cytokine response modifier A (CrmA), which inhibits caspase‐1 and ‐8, still undergo hypericin‐induced apoptosis and necrosis. Evidence is provided for the release of mitochondrial cytochrome c in the cytosol and for procaspase‐3 activation in the hypericin‐induced cell killing.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(98)01416-1
Language:
English
Publisher:
Wiley
Publication Date:
1998
detail.hit.zdb_id:
1460391-3
SSG:
12
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