In:
Journal of Clinical Laboratory Analysis, Wiley, Vol. 30, No. 6 ( 2016-11), p. 924-929
Abstract:
The whole blood extraction for liquid chromatography tandem mass spectrometry (LC‐MS/MS) of simultaneous quantification of cyclosporine A (Cys A), tacrolimus (Tacrs), sirolimus (Siros), and everolimus (Evers) is still performed manually in many laboratories. The analytical results obtained with an automated method using a liquid handler versus a classical manual preparation were compared. Methods Cys A ( n = 36), Tacrs ( n = 50), Siros ( n = 34), Evers ( n = 38) whole blood samples of patients were analyzed by LC‐MS/MS assay after manual preparation and automated process using a liquid handling platform including a centrifugation step. Results The comparison between manual and automated extraction investigated by a linear regression showed a high correlation between results [(Tacrs “automated”) = 1.0927 × (Tacrs “manual”) − 0.36; (Cys A “automated”) = 1.0284 × (Cys A “manual”) + 0.0312; (Siros “automated”) = 0.9923 × (Siros “manual”) + 0.4001; (Evers “automated”) = 1.0000 × (Evers “manual”) − 0.0600]. Conclusion The results obtained by the automated and manual preparation are consistent. The automated method is applied for high‐throughput therapeutic drug monitoring of immunosuppressive drugs in routine practice, leading to an increase in quality.
Type of Medium:
Online Resource
ISSN:
0887-8013
,
1098-2825
DOI:
10.1002/jcla.2016.30.issue-6
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2001635-9
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