GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Protein Isolation and Separation Techniques of Pasteurella multocidavia One- and Two-Dimen-Sional Gel Electrophoresis
    Radiance Research Academy ; 2022
    In:  International Journal of Current Research and Review Vol. 14, No. 12 ( 2022), p. 01-08
    Details
    In: International Journal of Current Research and Review, Radiance Research Academy, Vol. 14, No. 12 ( 2022), p. 01-08
    Abstract: Introduction: Bacteria in the genus Pasteurella live as commensal parasites on the mucous membranes of vertebrates, particularly mammals and birds. Pasteurella species like Pasteurella multocida and Pasteurella haemolytica cause significant economic losses as a result of diseases caused by these two microorganisms. Objective: This study compares protein isolation and analysis methods for effective one and two-dimensional gel electrophoresis of Pasteurella multocidaproteins and a preliminary protein mapping and analysis of P. multocida serotype B. Methodology: Protein samples were obtained by two isolation methods: homogenisation/sonication and detergent lysis. 1D SDS-PAGE methodology was optimized for protein loading, separating gel percentages and gel size. Detergent lysis was the preferred isolation method of total proteins. Results: The optimum running condition for SDS-PAGE was determined to be 15 µg of protein loading run on a 12.5% separating gel with a gel size of 10cm x 10cm. Preliminary 1D SDS-PAGE analysis revealed 3 distinct protein bands (33 kDa, 39 kDa and 〉 200 kDa), which could only be found specifically in serotype B samples. The samples were then separated by 2-DGE after optimization. The optimum running condition was 500µg of protein sample loaded using rehydration loading with immobilized pH gradient (IPG) strips pH 3–10 for the first-dimension isoelectric focusing (IEF) and 12.5% single percentage gel for the seconddimension SDS-PAGE. The protein map, which produced the most, spots were compared to in silico 2-DGE protein patterns of Pm70 in GELBANK database. Conclusion: A total of 23 proteins were identified and protein of 33 kDA was identified in both 1D SDS-PAGE and 2-DGE. This is the first protein analysis and the first protein map of P. multocida serotype B ever produced.
    Type of Medium: Online Resource
    ISSN: 2231-2196 , 0975-5241
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.31782/IJCRR.2022
    DOI: 10.31782/IJCRR
    DOI: 10.31782/IJCRR.2022.141208
    Language: Unknown
    Publisher: Radiance Research Academy
    Publication Date: 2022
    detail.hit.zdb_id: 2620630-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Chitinase Activity by Chitin Degrading Strain (Bacillus Salmalaya) in Shrimp Waste
    Radiance Research Academy ; 2022
    In:  International Journal of Current Research and Review Vol. 14, No. 11 ( 2022), p. 11-17
    Details
    In: International Journal of Current Research and Review, Radiance Research Academy, Vol. 14, No. 11 ( 2022), p. 11-17
    Abstract: Introduction: Chitin degradation by chitinase enzyme can be used on a large scale for bioremediation of seafood waste and is environmentally friendly. Objective: The main aim of this study was to screen the potential of the strain as a chitin degrading agent. Methods: In this present study, the cell-free supernatant of Bacillus salmalaya was determined for its protein concentration. Bacillus salmalaya139SI was isolated from agricultural soil and it was identified by staining technique and colony morphology. The production of chitinase by Bacillus salmalaya139SI was optimized under different concentrations of substrate, pH and temperature. Results: Strain 139SI exhibited strong hemolytic activity and the crude protein concentration of Bacillus salmalaya was 84.09mg/ mL with OD value 0.462. Strain 139SI were also screened on colloidal chitin agar medium supplemented with mineral salt. Chikinase production was determined by clear zones of hydrolysis produced after 7 days of incubation at 37°C. The maximum chitinase production was observed in Brain Heart Infusion broth supplemented with 1.0% colloidal chitin at pH 7 and temperature 35°C after four days of incubation. Chitinase activity was observed when high concentrations of crude extract of 139SI able to degrade shrimp shell by showing the degradation zone at day 4. Conclusion: From the results, we concluded that the Bacillus salmalayahas potential to be a biofunctional chitinase that could degrade complex polysaccharides present in the organic wastes and applicable in cleaning the environment.
    Type of Medium: Online Resource
    ISSN: 2231-2196 , 0975-5241
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.31782/IJCRR.2022
    DOI: 10.31782/IJCRR
    DOI: 10.31782/IJCRR.2022.141107
    Language: Unknown
    Publisher: Radiance Research Academy
    Publication Date: 2022
    detail.hit.zdb_id: 2620630-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Green cleaning activity of Bacillus salmalaya 139Sl: a novel strain for removing common household stains
    Springer Science and Business Media LLC ; 2022
    In:  Biomass Conversion and Biorefinery
    Details
    In: Biomass Conversion and Biorefinery, Springer Science and Business Media LLC
    Type of Medium: Online Resource
    ISSN: 2190-6815 , 2190-6823
    URL: Article
    DOI: 10.1007/s13399-022-03147-z
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2592298-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Pathological study of Pasteurella Multocida Recombinant Clone ABA392
    Lahore Medical and Dental College ; 2022
    In:  Pakistan Journal of Medical and Health Sciences Vol. 16, No. 2 ( 2022-02-26), p. 1112-1116
    Details
    In: Pakistan Journal of Medical and Health Sciences, Lahore Medical and Dental College, Vol. 16, No. 2 ( 2022-02-26), p. 1112-1116
    Abstract: Objective: In current study Molecular analysis of the recombinant clone ABA392 was carried out Methodology. In this study, detailed molecular analysis of the recombinant clone ABA392 were carried out by examining plasmid stability, plasmid profiling, determination of the plasmid molecular weight, and restriction endonuclease analysis. Results: From the experiments above, the plasmid size of ABA392 was estimated as 3.5 kb, while the size of the insert gene of ABA392 was estimated as 0.8 kb (double digested with PstI and SmaI restriction enzymes). A Pathogenicity study of recombinant clone ABA392 was carried out via a mouse virulence test and histopathological analysis, where it was found that mice, which were challenged with ABA392 and the parent strain of Pasteurella multocida serotype B strain PMB202, were lethal within 24 to 72 hours upon inoculation. Based on microscopic examination, the virulence effect was observed by marked haemorrhage in lung, liver, spleen, and kidney tissues. Conclusion: This study has also proven that the plasmid of recombinant clone ABA392 was stably maintained in the Escherichia coli JM101 host system as reported previously. A preliminary protein analysis via SDS-PAGE was performed and a distinct protein band was most likely to be detected, which was approximately 16kD. Nonetheless, further characterization of the recombinant clone ABA392 proteins needs to be done to determine the nature of these proteins, which are involved in the virulence mechanism of hemorrhagic septicemia. Keywords: Plasmid profiling; Pathogenicity; recombinant clone; Pasteurella multocida
    Type of Medium: Online Resource
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.53350/pjmhs22162
    DOI: 10.53350/pjmhs2216
    DOI: 10.53350/pjmhs221621112
    Language: Unknown
    Publisher: Lahore Medical and Dental College
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Analysis and Characterization of Chitinase in Bacillus salmalaya Strain 139SI
    Radiance Research Academy ; 2022
    In:  International Journal of Current Research and Review Vol. 14, No. 11 ( 2022), p. 31-36
    Details
    In: International Journal of Current Research and Review, Radiance Research Academy, Vol. 14, No. 11 ( 2022), p. 31-36
    Abstract: Introduction: Chitinases are enzymes that hydrolyze the internal β-1,4 glycosidic linkages of chitin, a significant structural component of arthropod exoskeletons and fungus cell walls. Objective: Main objective of current study was to determine the protein and to check the antimicrobial, antifungal activity of chitinase in Bacillus salmalaya strain 139SI. Methodology: Purified and estimation enzymes were quantified by the method of Lowry method. Antimicrobial action of chitinase was done using standard Disc Diffusion method while the hyphal extension inhibition assay was used to test the antifungal activity of pure chitinase strains 139SI, 140SI, and 141SI. Results: In this study, Bacillus salmalaya 139SIexhibited strong hemolytic activity and their protein concentration was measured as 56.43 mg/mL. In addition, strain 139SI had strong antifungal activity against phytopathogenic fungus including Fusarium sp., R. solani and Phytophthora sp. Strain 139SI had the capability in degrading the peptidoglycan component of cell walls of gram-negative bacteria such as Escherichia coli but not against the gram-positive, Staphylococcus aureus. Chitinase activity was observed when 200μl crude extract of 139SI able to degrade 0.09 g chitin of shrimp shell by breaking down shrimp shell structure and bonds of chitin effectively as early as 2 days or up to 7 days. Conclusion: Hence, based on the results, B. salmalaya 139SI has potential to be a novel biofunctional chitinase that could use as a biological agent in degrading the chitin component of fungal cell walls and shells waste of many kind of insect and crustaceans for solving future problem in the agricultural sector and fishery industry.
    Type of Medium: Online Resource
    ISSN: 2231-2196 , 0975-5241
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.31782/IJCRR.2022
    DOI: 10.31782/IJCRR
    DOI: 10.31782/IJCRR.2022.141108
    Language: Unknown
    Publisher: Radiance Research Academy
    Publication Date: 2022
    detail.hit.zdb_id: 2620630-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Enhancing Lipase Production of Bacillus salmalaya Strain 139SI Using Different Carbon Sources and Surfactants
    MDPI AG ; 2022
    In:  Applied Microbiology Vol. 2, No. 1 ( 2022-02-28), p. 237-247
    Details
    In: Applied Microbiology, MDPI AG, Vol. 2, No. 1 ( 2022-02-28), p. 237-247
    Abstract: Microbial lipase is one of the major sources of the enzyme that has been broadly exploited in the food, detergent, and pharmaceutical industries due to its high catalytic activity, high yield, and environmental friendliness and cost-effectiveness. Therefore, the aim of this study was to optimize the medium for the submerged fermentation for lipase production by a novel strain, Bacillus salmalaya strain 139SI. The media subjected to lipase production was Luria Bertani (LB) with different carbon sources and surfactants supplemented to determine which would give the highest lipase activity of Bacillus salmalaya. The Lipase activity of the supernatant containing lipase enzyme was ddetermined using the titrimetric method with hydrolysis reaction. Results showed that the olive oil that was used as a carbon source, induced the highest lipase activity (11.0 U/mL) compared to sunflower oil (9.6 U/mL) and cooking oil waste (7.8 U/mL). For surfactants, LB medium supplemented with tween 80 enhanced higher lipase activity (6.8 U/mL) compared to tween 20 (6.0 U/mL) and sodium dodecyl sulphate (SDS) (2.0 U/mL). Thus, it can be concluded that submerged fermentation allows optimization of the culture medium whereby, among carbon sources, olive oil induced the highest lipase production, whereas Tween 80 was the best lipase inducer compared to other surfactants.
    Type of Medium: Online Resource
    ISSN: 2673-8007
    URL: Article
    DOI: 10.3390/applmicrobiol2010017
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 3136474-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Biosurfactant Screening and Antibiotic Analysis of Bacillus salmalaya
    Radiance Research Academy ; 2022
    In:  International Journal of Current Research and Review Vol. 14, No. 12 ( 2022), p. 56-64
    Details
    In: International Journal of Current Research and Review, Radiance Research Academy, Vol. 14, No. 12 ( 2022), p. 56-64
    Abstract: Introduction: Biosurfactants are made up of naturally occurring molecules such as lipopeptide, glycoprotein, lipoprotein, and fatty acids. Using biosurfactants rather than chemical and synthetic surfactants is safer. Objective: The aim of this study was to investigate biosurfactant properties and antibiotic resistance of soil bacteria, Bacillus salmalaya strain 139SI. Bacillus salmalaya produces biosurfactants, which capable of reducing surface tension of certain media, as they are surface-active molecules. Methods: In current study Parafilm M Test, Drop Collapse, Oil spreading methods for biosurfactants screening were used and antibiotic analysis was done using an aseptic technique. Results: 600μl biosurfactant (460 mg/ml) was the most effective volume to produce large diameter of oil spreading zone when 700 μl of oil was used. 0.23g freeze-dried supernatant powder mixed with 0.5 ml distilled water (460 mg/ml) produced larger clearing zone than powder mixed with 1 ml (230 mg/ml) and 1.5 ml distilled water (153 mg/ml). In cleaning activity of crude oil container, biosurfactant was able to clean oil in the container and this can be applied in cleaning oil waste from crude oil tank and can also be used to clean oil spillage. In antibiotic analysis study, ten soil bacteria isolates included Bacillus salmalaya were selected. Conclusion: All isolates had shown different sensitivity towards different antibiotics. Bacillus salmalaya showed sensitivity to almost all selected antibiotic compared to other isolates. This indicated that this strain is safe to be used as it was sensitive to many antibiotics.
    Type of Medium: Online Resource
    ISSN: 2231-2196 , 0975-5241
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.31782/IJCRR.2022
    DOI: 10.31782/IJCRR
    DOI: 10.31782/IJCRR.2022.141209
    Language: Unknown
    Publisher: Radiance Research Academy
    Publication Date: 2022
    detail.hit.zdb_id: 2620630-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Screening of Anticancer and Immunomodulatory Properties of Recombinant pQE-HAS113 Clone Derived from Streptococcus Equi
    Lahore Medical and Dental College ; 2022
    In:  Pakistan Journal of Medical and Health Sciences Vol. 16, No. 2 ( 2022-02-26), p. 1100-1103
    Details
    In: Pakistan Journal of Medical and Health Sciences, Lahore Medical and Dental College, Vol. 16, No. 2 ( 2022-02-26), p. 1100-1103
    Abstract: Objective: Streptococcus equi has been identified to contain the hyaluronan synthase gene (HAS), which could have a role in the cell proliferation activity. The objective of this research was to determine anticancer properties of HAS113 recombinant clone. Methodology: The intracellular and extracellular portions of the expressed gene products of pQE-HAS113 were harvested and evaluate for anticancer properties against prostate cancer cell (PC3) and breast cancer cell (MCF-7). To evaluate anti-proliferative properties of pQE-HAS113, two assays were carried out: MTT assay, which was used for assessing percent cell inhibition and apoptosis assay, through the AO/EB stain of living, apoptotic, as well as necrotic cells. For evaluation of the immunomodulatory properties of pQE-HAS113 on RAW264.7 in terms of IL-6, IFN-γ & IL-8 expression, flow cytometry examination was done. The harvested intracellular and extracellular portions of pQE-HAS113 were tested. Results: The results indicated that the percentage proliferation of both the prostate cancer and breast cancer cells were 2.1% and 7.2% respectively against the intracellular portions of pQE-HAS113. While in case of the extracellular portions of pQE-HAS113 the proliferation rate of both the prostate cancer and breast cancer cells were 6.5% and 6.7% respectively. The PQE-HAS113 intracellular clone gene has shown proinflammatory activity by stimulating the expression of IL-6, INF-γ, & IL-8 in the RAW264.7 macrophage cells. Conclusion: Thus, the results from the present study indicated that the expressed product of recombinant clone of HAS 113, pQE-HAS113 has potential to be used as anti-proliferative and apoptosis inducing substance for prostate and breast cancer. Keywords: Streptococcus equi; breast cancer; cell proliferation; apoptosis ass
    Type of Medium: Online Resource
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.53350/pjmhs22162
    DOI: 10.53350/pjmhs2216
    DOI: 10.53350/pjmhs221621100
    Language: Unknown
    Publisher: Lahore Medical and Dental College
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...