In:
BioMed Research International, Hindawi Limited, Vol. 2015 ( 2015), p. 1-7
Abstract:
The aim of the study was to examine the association between polymorphisms of DNA repair genes and chromosomal damage of 1,3-butadiene- (BD-) exposed workers. The study was conducted in 45 pairs of occupationally exposed workers in a BD product workshop and matched control workers in an administrative office and a circulatory water workshop in China. Newly developed biomarkers (micronuclei, MNi; nucleoplasmic bridges, NPBs; nuclear buds, NBUDs) in the cytokinesis-blocked micronucleus (CBMN) cytome assay were adopted to detect chromosomal damage. PCR and PCR-restriction fragment length polymorphism (RFLP) are adopted to analyze polymorphisms of DNA repair genes, such as X-ray repair cross-complementing Group 1 ( XRCC1 ), O 6 -methylguanine-DNA methyltransferase ( MGMT ), poly (adenosine diphosphate-ribose) polymerases ( ADPRT ), and apurinic/apyrimidinic endonucleases ( APE1 ). The BD-exposed workers exhibited increased frequencies of MNi and NPBs when compared to subjects in the control group. The results also show that the BD-exposed workers carrying XRCC1 diplotypes TCGA-CCGG ( 4.25 ± 2.06 ‰ ) ( FR = 2.10 , 95% CI: 1.03–4.28) and TCGG-TCGA ( 5.80 ± 3.56 ‰ ) ( FR = 2.75 , 95% CI: 0.76–2.65) had statistically higher NBUD frequencies than those who carried diplotype TCGG-TCGG ( 1.89 ± 1.27 ‰ ). Our study suggests that polymorphisms of XRCC1 gene may influence chromosomal damage in BD-exposed workers.
Type of Medium:
Online Resource
ISSN:
2314-6133
,
2314-6141
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2015
detail.hit.zdb_id:
2698540-8
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