In:
Contrast Media & Molecular Imaging, Hindawi Limited, Vol. 2018 ( 2018), p. 1-9
Abstract:
Objective. To synthesize 68 Ga-Glu-urea-Lys(Ahx)-HBED-CC ( 68 Ga-PSMA-11) with a synthesis module and investigate PET-CT imaging to monitor PSMA expression during prostate cancer (PCa) progression and tumor growth in mice bearing subcutaneous PCa xenografts. Method. The radiochemical purity and stability of 68 Ga-PSMA-11 were determined via radio-HPLC. The PCa cell lines of different PSMA expression levels (PC3, VCAP±, CWR22RV1+, and LNCaP++) were selected to mimic the PCa progression. 68 Ga-PSMA-11 biodistribution was studied by dissection method and in vivo imaging with micro PET-CT. The expression levels of PSMA in tumor cells and tissues were analyzed by immunofluorescence, flow cytometry, and western blot. The correlation between PSMA expression and radio-uptake was also evaluated. 2-PMPA preadministration served as a block group. Results. The radiochemical purity of 68 Ga-PSMA-11 was 99.6 ± 0.1% and stable in vitro for 2 h. The equilibrium binding constant (Kd) of 68 Ga-PSMA-11 to LNCaP, CWR22Rv1, PC-3, and VCAP cells was 4.3 ± 0.8 nM, 16.4 ± 1.3 nM, 225.3 ± 20.8 nM, and 125.6 ± 13.1 nM, respectively. Results of tumor uptake (% ID and % ID/g or % ID/cm 3 ) of 68 Ga-PSMA-11 in biodistribution and micro PET imaging were LNCaP 〉 CWR22RV1 〉 PC-3 and VCAP due to different PSMA expression levels. It was confirmed by flow cytometry, western blot, and immunofluorescence. Tumor uptake (% ID/cm 3 ) of 68 Ga-PSMA-11 increased with the tumor anatomical volume in quadratic polynomial fashion and reached the peak (when tumor volume was 0.5 cm 3 ) earlier than tumor uptake (% ID). Tumor uptake (% ID/cm 3 ) of 68 Ga-PSMA-11 based on functional volume correlated well with the PSMA expression in a linear manner ( y = 9.35 x + 2.59 , R 2 = 0.8924 , and p 〈 0.0001 ); however, low dose 2-PMPA causes rapid renal clearance of increased tumor/kidney uptake of 68 Ga-PSMA-11. Conclusions. The 68 Ga-PSMA-11 PET-CT imaging could invasively evaluate PSMA expression during PCa progression and tumor growth with % ID/cm 3 (based on functional volume) as an important index. Low dose 2-PMPA preadministration might be a choice to decrease kidney uptake of 68 Ga-PSMA-11.
Type of Medium:
Online Resource
ISSN:
1555-4309
,
1555-4317
DOI:
10.1155/2018/8046541
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2018
detail.hit.zdb_id:
2222967-X
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