GLORIA — GEOMAR Library Ocean Research Information Access

Hits per page

hits 1 - 3 | 3 hits

Sorting

Online Resource

RNA sequencing provides exquisite insight into the manipulation of the alveolar macrophage by tubercle bacilli

Nalpas, Nicolas C. ; Magee, David A. ; Conlon, Kevin M. ; [et al.]

Springer Science and Business Media LLC ; 2015

In: Scientific Reports Vol. 5, No. 1 ( 2015-09-08)

add to mindlist on the mindlist

Details

In: Scientific Reports, Springer Science and Business Media LLC, Vol. 5, No. 1 ( 2015-09-08)

Abstract: Mycobacterium bovis , the agent of bovine tuberculosis, causes an estimated $3 billion annual losses to global agriculture due, in part, to the limitations of current diagnostics. Development of next-generation diagnostics requires a greater understanding of the interaction between the pathogen and the bovine host. Therefore, to explore the early response of the alveolar macrophage to infection, we report the first application of RNA-sequencing to define, in exquisite detail, the transcriptomes of M. bovis -infected and non-infected alveolar macrophages from ten calves at 2, 6, 24 and 48 hours post-infection. Differentially expressed sense genes were detected at these time points that revealed enrichment of innate immune signalling functions and transcriptional suppression of host defence mechanisms ( e.g ., lysosome maturation). We also detected differentially expressed natural antisense transcripts, which may play a role in subverting innate immune mechanisms following infection. Furthermore, we report differential expression of novel bovine genes, some of which have immune-related functions based on orthology with human proteins. This is the first in-depth transcriptomics investigation of the alveolar macrophage response to the early stages of M. bovis infection and reveals complex patterns of gene expression and regulation that underlie the immunomodulatory mechanisms used by M. bovis to evade host defence mechanisms.

Type of Medium: Online Resource

ISSN: 2045-2322

URL: Article

DOI: 10.1038/srep13629

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2015

detail.hit.zdb_id: 2615211-3

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

Online Resource

Global Gene Expression and Systems Biology Analysis of Bovine Monocyte-Derived Macrophages in Response to In Vitro Challenge with Mycobacterium bovis

Magee, David A. ; Taraktsoglou, Maria ; Killick, Kate E. ; [et al.]

Public Library of Science (PLoS) ; 2012

In: PLoS ONE Vol. 7, No. 2 ( 2012-2-22), p. e32034-

add to mindlist on the mindlist

Details

In: PLoS ONE, Public Library of Science (PLoS), Vol. 7, No. 2 ( 2012-2-22), p. e32034-

Type of Medium: Online Resource

ISSN: 1932-6203

URL: Article

DOI: 10.1371/journal.pone.0032034

DOI: 10.1371/journal.pone.0032034.g001

DOI: 10.1371/journal.pone.0032034.g002

DOI: 10.1371/journal.pone.0032034.g003

DOI: 10.1371/journal.pone.0032034.g004

DOI: 10.1371/journal.pone.0032034.g005

DOI: 10.1371/journal.pone.0032034.g006

DOI: 10.1371/journal.pone.0032034.g007

DOI: 10.1371/journal.pone.0032034.s001

DOI: 10.1371/journal.pone.0032034.s002

DOI: 10.1371/journal.pone.0032034.s003

DOI: 10.1371/journal.pone.0032034.s004

DOI: 10.1371/journal.pone.0032034.s005

DOI: 10.1371/journal.pone.0032034.s006

DOI: 10.1371/journal.pone.0032034.s007

DOI: 10.1371/journal.pone.0032034.s008

DOI: 10.1371/journal.pone.0032034.s009

DOI: 10.1371/journal.pone.0032034.s010

DOI: 10.1371/journal.pone.0032034.s011

DOI: 10.1371/journal.pone.0032034.s012

DOI: 10.1371/journal.pone.0032034.s013

DOI: 10.1371/journal.pone.0032034.s014

DOI: 10.1371/journal.pone.0032034.s015

Language: English

Publisher: Public Library of Science (PLoS)

Publication Date: 2012

detail.hit.zdb_id: 2267670-3

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

Online Resource

Key Hub and Bottleneck Genes Differentiate the Macrophage Response to Virulent and Attenuated Mycobacterium bovis

Killick, Kate E. ; Magee, David A. ; Park, Stephen D. E. ; [et al.]

Frontiers Media SA ; 2014

In: Frontiers in Immunology Vol. 5 ( 2014-10-01)

add to mindlist on the mindlist

Details

In: Frontiers in Immunology, Frontiers Media SA, Vol. 5 ( 2014-10-01)

Type of Medium: Online Resource

ISSN: 1664-3224

URL: Article

DOI: 10.3389/fimmu.2014.00422

Language: Unknown

Publisher: Frontiers Media SA

Publication Date: 2014

detail.hit.zdb_id: 2606827-8

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

hits 1 - 3 | 3 hits