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  • 1
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2023-01-20)
    Abstract: The connection between Pediatric Inflammatory Multisystem Syndrome (PIMS) and Kawasaki Disease (KD) is not yet fully understood. Using the same national registry, clinical features and outcome of children hospitalized in Germany, and Innsbruck (Austria) were compared. Reported to the registry were 395 PIMS and 69 KD hospitalized patients. Patient age in PIMS cases was higher than in KD cases (median 7 [IQR 4–11] vs. 3 [IQR 1–4] years). A majority of both PIMS and KD patients were male and without comorbidities. PIMS patients more frequently presented with organ dysfunction, with the gastrointestinal (80%), cardiovascular (74%), and respiratory (52%) systems being most commonly affected. By contrast, KD patients more often displayed dermatological (99% vs. 68%) and mucosal changes (94% vs. 64%), plus cervical lymph node swelling (51% vs. 34%). Intensive care admission (48% vs. 19%), pulmonary support (32% vs. 10%), and use of inotropes/vasodilators (28% vs. 3%) were higher among PIMS cases. No patients died. Upon patient discharge, potentially irreversible sequelae—mainly cardiovascular—were reported (7% PIMS vs. 12% KD). Despite differences in age distribution and disease severity, PIMS and KD cases shared many common clinical and prognostic characteristics. This supports the hypothesis that the two entities represent a syndrome continuum.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2615211-3
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  • 2
    In: Genomics, Proteomics & Bioinformatics, Oxford University Press (OUP), Vol. 20, No. 2 ( 2022-04-01), p. 405-417
    Abstract: High-quality DNA extraction is a crucial step in metagenomic studies. Bias by different isolation kits impairs the comparison across datasets. A trending topic is, however, the analysis of multiple metagenomes from the same patients to draw a holistic picture of microbiota associated with diseases. We thus collected bile, stool, saliva, plaque, sputum, and conjunctival swab samples and performed DNA extraction with three commercial kits. For each combination of the specimen type and DNA extraction kit, 20-gigabase (Gb) metagenomic data were generated using short-read sequencing. While profiles of the specimen types showed close proximity to each other, we observed notable differences in the alpha diversity and composition of the microbiota depending on the DNA extraction kits. No kit outperformed all selected kits on every specimen. We reached consistently good results using the Qiagen QiAamp DNA Microbiome Kit. Depending on the specimen, our data indicate that over 10 Gb of sequencing data are required to achieve sufficient resolution, but DNA-based identification is superior to identification by mass spectrometry. Finally, long-read nanopore sequencing confirmed the results (correlation coefficient & gt; 0.98). Our results thus suggest using a strategy with only one kit for studies aiming for a direct comparison of multiple microbiotas from the same patients.
    Type of Medium: Online Resource
    ISSN: 1672-0229 , 2210-3244
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 2233708-8
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  • 3
    In: Geoscientific Model Development, Copernicus GmbH, Vol. 15, No. 22 ( 2022-11-25), p. 8613-8638
    Abstract: Abstract. While advanced computational capabilities have enabled the development of complex ocean general circulation models (OGCMs) for marginal seas, systematic comparisons of regional ocean models and their setups are still rare. The Baltic Sea Model Intercomparison Project (BMIP), introduced herein, was therefore established as a platform for the scientific analysis and systematic comparison of Baltic Sea models. The inclusion of a physically consistent regional reanalysis data set for the period 1961–2018 allows for standardized meteorological forcing and river runoff. Protocols to harmonize model outputs and analyses are provided as well. An analysis of six simulations performed with four regional OGCMs differing in their resolution, grid coordinates, and numerical methods was carried out to explore intermodel differences despite harmonized forcing. Uncertainties in the modeled surface temperatures were shown to be larger at extreme than at moderate temperatures. In addition, a roughly linear increase in the temperature spread with increasing water depth was determined and indicated larger uncertainties in the near-bottom layer. On the seasonal scale, the model spread was larger in summer than in winter, likely due to differences in the models' thermocline dynamics. In winter, stronger air–sea heat fluxes and vigorous convective and wind mixing reduced the intermodel spread. Uncertainties were likewise reduced near the coasts, where the impact of meteorological forcing was stronger. The uncertainties were highest in the Bothnian Sea and Bothnian Bay, attributable to the differences between the models in the seasonal cycles of sea ice triggered by the ice–albedo feedback. However, despite the large spreads in the mean climatologies, high interannual correlations between the sea surface temperatures (SSTs) of all models and data derived from a satellite product were determined. The exceptions were the Bothnian Sea and Bothnian Bay, where the correlation dropped significantly, likely related to the effect of sea ice on air–sea heat exchange. The spread of water salinity across the models is generally larger compared to water temperature, which is most obvious in the long-term time series of deepwater salinity. The inflow dynamics of saline water from the North Sea is covered well by most models, but the magnitude, as inferred from salinity, differs as much as the simulated mean salinity of deepwater. Marine heat waves (MHWs), coastal upwelling, and stratification were also assessed. In all models, MHWs were more frequent in shallow areas and in regions with seasonal ice cover. An increase in the frequency (regionally varying between ∼50 % and 250 %) and duration (50 %–150 %) of MHWs during the last 3 decades in all models was found as well. The uncertainties were highest in the Bothnian Bay, likely due to the different trends in sea ice presence. All but one of the analyzed models overestimated upwelling frequencies along the Swedish coast, the Gulf of Finland, and around Gotland, while they underestimated upwelling in the Gulf of Riga. The onset and seasonal cycle of thermal stratification likewise differed among the models. Compared to observation-based estimates, in all models the thermocline in early spring was too deep, whereas a good match was obtained in June when the thermocline intensifies.
    Type of Medium: Online Resource
    ISSN: 1991-9603
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2022
    detail.hit.zdb_id: 2456725-5
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  • 4
    In: Geoscientific Model Development, Copernicus GmbH, Vol. 15, No. 7 ( 2022-04-08), p. 2973-3020
    Abstract: Abstract. The Earth system model EC-Earth3 for contributions to CMIP6 is documented here, with its flexible coupling framework, major model configurations, a methodology for ensuring the simulations are comparable across different high-performance computing (HPC) systems, and with the physical performance of base configurations over the historical period. The variety of possible configurations and sub-models reflects the broad interests in the EC-Earth community. EC-Earth3 key performance metrics demonstrate physical behavior and biases well within the frame known from recent CMIP models. With improved physical and dynamic features, new Earth system model (ESM) components, community tools, and largely improved physical performance compared to the CMIP5 version, EC-Earth3 represents a clear step forward for the only European community ESM. We demonstrate here that EC-Earth3 is suited for a range of tasks in CMIP6 and beyond.
    Type of Medium: Online Resource
    ISSN: 1991-9603
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2022
    detail.hit.zdb_id: 2456725-5
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  • 5
    Online Resource
    Online Resource
    Copernicus GmbH ; 2022
    In:  Earth System Dynamics Vol. 13, No. 1 ( 2022-03-30), p. 613-631
    In: Earth System Dynamics, Copernicus GmbH, Vol. 13, No. 1 ( 2022-03-30), p. 613-631
    Abstract: Abstract. Atmospheric rivers (ARs) are important drivers of hazardous precipitation levels and are often associated with intense floods. So far, the response of ARs to climate change in Europe has been investigated using global climate models within the CMIP5 framework. However, the spatial resolution of those models (1–3∘) is too coarse for an adequate assessment of local to regional precipitation patterns. Using a regional climate model with 0.22∘ resolution, we downscaled an ensemble consisting of 1 ERA-Interim (ERAI) reanalysis data hindcast simulation, 9 global historical, and 24 climate scenario simulations following greenhouse gas emission scenarios RCP2.6, RCP4.5, and RCP8.5. The performance of the climate model to simulate AR frequencies and AR-induced precipitation was tested against ERAI. Overall, we find a good agreement between the downscaled CMIP5 historical simulations and ERAI. However, the downscaled simulations better represented small-scale spatial characteristics. This was most evident over the terrain of the Iberian Peninsula, where the AR-induced precipitation pattern clearly reflected prominent east–west topographical elements, resulting in zonal bands of high and low AR impact. Over central Europe, the models simulated a smaller propagation distance of ARs toward eastern Europe than obtained using the ERAI data. Our models showed that ARs in a future warmer climate will be more frequent and more intense, especially in the higher-emission scenarios (RCP4.5, RCP8.5). However, assuming low emissions (RCP2.6), the related changes can be mostly mitigated. According to the high-emission scenario RCP8.5, AR-induced precipitation will increase by 20 %–40 % in western central Europe, whereas mean precipitation rates increase by a maximum of only 12 %. Over the Iberian Peninsula, AR-induced precipitation will slightly decrease (∼6 %) but the decrease in the mean rate will be larger (∼15 %). These changes will lead to an overall increased fractional contribution of ARs to heavy precipitation, with the greatest impact over the Iberian Peninsula (15 %–30 %) and western France (∼15 %). Likewise, the fractional share of yearly maximum precipitation attributable to ARs will increase over the Iberian Peninsula, the UK, and western France. Over Norway, average AR precipitation rates will decline by −5 % to −30 %, most likely due to dynamic changes, with ARs originating from latitudes 〉 60∘ N decreasing by up to 20 % and those originating south of 45∘ N increasing. This suggests that ARs over Norway will follow longer routes over the continent, such that additional moisture uptake will be impeded. By contrast, ARs from 〉60∘ N will take up moisture from the North Atlantic before making landfall over Norway. The found changes in the local AR pathway are probably driven by larger-scale circulation changes such as a change in dominating weather regimes and/or changes in the winter storm track over the North Atlantic.
    Type of Medium: Online Resource
    ISSN: 2190-4987
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2022
    detail.hit.zdb_id: 2578793-7
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  • 6
    In: Earth System Dynamics, Copernicus GmbH, Vol. 13, No. 2 ( 2022-04-13), p. 809-831
    Abstract: Abstract. We investigate the effect of variable marine biogeochemical light absorption on Indian Ocean sea surface temperature (SST) and how this affects the South Asian climate. In twin experiments with a regional Earth system model, we found that the average SST is lower over most of the domain when variable marine biogeochemical light absorption is taken into account, compared to the reference experiment with a constant light attenuation coefficient equal to 0.06 m−1. The most significant deviations (more than 1 ∘C) in SST are observed in the monsoon season. A considerable cooling of subsurface layers occurs, and the thermocline shifts upward in the experiment with the activated biogeochemical impact. Also, the phytoplankton primary production becomes higher, especially during periods of winter and summer phytoplankton blooms. The effect of altered SST variability on climate was investigated by coupling the ocean models to a regional atmosphere model. We find the largest effects on the amount of precipitation, particularly during the monsoon season. In the Arabian Sea, the reduction of the transport of humidity across the Equator leads to a reduction of the large-scale precipitation in the eastern part of the basin, reinforcing the reduction of the convective precipitation. In the Bay of Bengal, it increases the large-scale precipitation, countering convective precipitation decline. Thus, the key impacts of including the full biogeochemical coupling with corresponding light attenuation, which in turn depends on variable chlorophyll a concentration, include the enhanced phytoplankton primary production, a shallower thermocline, and decreased SST and water temperature in subsurface layers, with cascading effects upon the model ocean physics which further translates into altered atmosphere dynamics.
    Type of Medium: Online Resource
    ISSN: 2190-4987
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2022
    detail.hit.zdb_id: 2578793-7
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  • 7
    In: RNA Biology, Informa UK Limited, Vol. 20, No. 1 ( 2023-12-31), p. 482-494
    Type of Medium: Online Resource
    ISSN: 1547-6286 , 1555-8584
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2159587-2
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  • 8
    In: Journal of Clinical Medicine, MDPI AG, Vol. 12, No. 10 ( 2023-05-16), p. 3502-
    Abstract: Background: Diabetes mellitus worsens outcomes in patients suffering from heart disease undergoing cardiac procedures. Objectives: To investigate the impact of diabetes in patients undergoing mitral transcatheter edge-to-edge repair (M-TEER). Methods: 1118 patients treated with M-TEER for functional (FMR) and degenerative (DMR) mitral regurgitation (MR) between 2010 and 2021 were analyzed using the combined endpoint of death/rehospitalization for heart failure (HFH). Results: Among diabetics (N = 306; 27.4%), comorbidities such as coronary artery disease (75.2% vs. 62.7%; p 〈 0.001) and progressed (stage III/IV) chronic kidney disease (79.5% vs. 72.6%; p = 0.018) were more frequent. The rate of FMR was higher in diabetics (71.9% vs. 64.5%; p 〈 0.001). The combined endpoint occurred more frequently in diabetics (40.2% vs. 35.6%; log-rank = 0.035). While no difference was observed in FMR patients (36.8% vs. 37.6%; log-rank p = 0.710), rates of the combined endpoint differed significantly between diabetics and non-diabetics in DMR patients (48.8% vs. 31.9%; log-rank p = 0.001) only. However, diabetes did neither predict the combined endpoint in the overall (OR: 0.97; 95% CI 0.65–1.45; p = 0.890) nor in the DMR cohort (OR: 0.73; 95% CI 0.35–1.51; p = 0.389). Among diabetics treated with M-TEER, troponin (OR: 2.32; 95% CI 1.3–3.7; p = 0.002) and estimated glomerular filtration rate (OR: 0.52; 95% CI 0.3–0.88; p = 0.018) independently predicted the combined endpoint. Conclusions: Diabetes is associated with adverse outcomes after M-TEER, particularly in DMR patients. However, diabetes does not predict the combined endpoint. In diabetics undergoing M-TEER, biochemical markers associated with organ function and damage independently predict the combined endpoint of death and rehospitalization.
    Type of Medium: Online Resource
    ISSN: 2077-0383
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662592-1
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  • 9
    In: Global Change Biology, Wiley, Vol. 28, No. 17 ( 2022-09), p. 5310-5319
    Abstract: Climate change influences the ocean's physical and biogeochemical conditions, causing additional pressures on marine environments and ecosystems, now and in the future. Such changes occur in environments that already today suffer under pressures from, for example, eutrophication, pollution, shipping, and more. We demonstrate how to implement climate change into regional marine spatial planning by introducing data of future temperature, salinity, and sea ice cover from regional ocean climate model projections to an existing cumulative impact model. This makes it possible to assess climate change impact in relation to pre‐existing cumulative impact from current human activities. Results indicate that end‐of‐century projected climate change alone is a threat of the same magnitude as the combination of all current pressures to the marine environment. These findings give marine planners and policymakers forewarning on how future climate change may impact marine ecosystems, across space, emission scenarios, and in relation to other pressures.
    Type of Medium: Online Resource
    ISSN: 1354-1013 , 1365-2486
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2020313-5
    SSG: 12
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  • 10
    In: HNO, Springer Science and Business Media LLC
    Abstract: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a type‑2 inflammatory disease of the upper airways, with severe impairment of quality of life. Persons affected by NSAID-exacerbated respiratory disease (NERD) usually present with highly dynamic recurrence of polyps and disease despite prior treatment with sinus surgeries, oral corticosteroids, and aspirin desensitization (ATAD). Biologic therapy has fundamentally changed the choice of therapeutic concept; however, limited data exist on subgroups such as NERD patients. The aim of the current article is to report on a multicenter retrospective study on add-on therapy with dupilumab, omalizumab, and mepolizumab in patients with NERD. Methods This is a retrospective cohort study of patients (NERD+, status after ATAD) in three reference centers in Germany (Munich, Mainz, Berlin). Subjective and objective parameters were collected at 4, 8, and 12 months after biologic therapy initiation in accordance with current EPOS/EUFOREA (European Position Paper on Rhinosinusitis and Nasal Polyps/European Forum for Research and Education in Allergy and Airway Diseases) guidelines. Biologic agents were chosen depending on availability and patient characteristics. Results Treatment was commenced in 122 patients meeting the criteria for CRSwNP and NERD. The endoscopic polyp score, SNOT-22 questionnaire score, visual analogue scoring of total symptoms/severity of disease, and sense of smell (psychophysical testing with Sniffin’Sticks/Brief Smell Identification Test, B‑SIT; Sensonics, Inc., Haddon Heights, NJ, USA) improved significantly after 4 and 12 months of add-on therapy ( p   〈 0.0001). All three biologic agents significantly improved one or more disease parameter. Adverse events were not life threatening but led to change of biologic agent in 4 cases. Patients rated biologic therapy significantly better than ATAD, with improved long-term disease control. Conclusion Add-on biologic therapy is effective, safe, and widely accepted among CRSwNP + NERD patients. Future studies might allow for personalized algorithms with sequential surgery, ATAD, and/or biologic therapy.
    Type of Medium: Online Resource
    ISSN: 0017-6192 , 1433-0458
    RVK:
    Language: German
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2024
    detail.hit.zdb_id: 1459183-2
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