In:
FEBS Letters, Wiley, Vol. 551, No. 1-3 ( 2003-09-11), p. 29-36
Abstract:
Human Bfl‐1 is an anti‐apoptotic Bcl‐2 family member. Here, we found that Bfl‐1 was converted into a potent death‐promoting protein by green fluorescent protein (GFP) fusion with its N‐terminus. The transient expression of GFP‐Bfl‐1 induced cytochrome c release and triggered apoptosis in 293T cells, which depended on the mitochondrial localization of GFP‐Bfl‐1. Apoptosis induced by GFP‐Bfl‐1 was significantly blocked by the pan‐caspase inhibitor carbobenzoxy‐Val‐Ala‐Asp‐fluoromethyl ketone, but was not blocked by either Bcl‐xL or Bfl‐1. Our findings provide a useful model for understanding the structural basis of Bcl‐2 family proteins that act in an opposite way despite sharing structural similarity between anti‐apoptotic and pro‐apoptotic proteins.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(03)00872-X
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
1460391-3
SSG:
12
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