In:
New Phytologist, Wiley, Vol. 200, No. 4 ( 2013-12), p. 1076-1088
Abstract:
Brassinosteroids ( BR s) are essential regulators of plant architecture. Understanding how BR s control plant height and leaf angle would facilitate development of new plant type varieties by biotechnology. A number of mutants involved in BR biosynthesis have been isolated but many of them lack detailed genetic analysis. Here, we report the isolation and characterization of a severe dwarf mutant, chromosome segment deleted dwarf 1 ( csdd1 ), which was deficient in BR biosynthesis in rice. We isolated the mutant by screening a tissue culture‐derived population, cloned the gene by mapping, and confirmed its function by complementary and RNA i experiments, combined with physiological and chemical analysis. We showed that the severe dwarf phenotype was caused by a complete deletion of a cytochrome P450 gene, CYP90D2/D2 , which was further confirmed in two independent T ‐ DNA insertion lines in different genetic backgrounds and by RNA interference. Our chemical analysis suggested that CYP90D2/D2 might catalyze C ‐3 dehydrogenation step in BR biosynthesis. We have demonstrated that the CYP90D2/D2 gene plays a more important role than previously reported. Allelic mutations of CYP90D2/D2 confer varying degrees of dwarfism and leaf angle, thus providing useful information for molecular breeding in grain crop plants.
Type of Medium:
Online Resource
ISSN:
0028-646X
,
1469-8137
DOI:
10.1111/nph.2013.200.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
208885-X
detail.hit.zdb_id:
1472194-6
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