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  • Wiley  (3)
  • Chen, Yulin  (3)
  • 1
    In: The FEBS Journal, Wiley, Vol. 286, No. 23 ( 2019-12), p. 4675-4692
    Abstract: The ability to alter single bases without homology directed repair ( HDR ) of double‐strand breaks provides a potential solution for editing livestock genomes for economic traits, which are often multigenic. Progress toward multiplex editing in large animals has been hampered by the costly inefficiencies of HDR via microinjection of in vitro manipulated embryos. Here, we designed sg RNA s to induce nonsense codons (C‐to‐T transitions) at four target sites in caprine FGF 5 , which is a crucial regulator of hair length in mammals. Initial transfections of the third generation Base Editor ( BE 3) plasmid and four different sg RNA s into caprine fibroblasts were ineffective in altering FGF 5 . In contrast, all five progenies produced from microinjected single‐cell embryos had alleles with a targeted nonsense mutation. The effectiveness of BE 3 to make single base changes varied considerably based on sg RNA design. In addition, the rate of mosaicism differed between animals, target sites, and tissue type. The phenotypic effects on hair fiber were characterized by hematoxylin and eosin, immunofluorescence staining, and western blotting. Differences in morphology were detectable, even though mosaicism was probably affecting the levels of FGF 5 expression. PCR amplicon and whole‐genome resequencing analyses for off‐target changes caused by BE 3 were low at a genome‐wide scale. This study provided the first evidence of base editing in large mammals produced from microinjected single‐cell embryos. Our results support further optimization of BE s for introgressing complex human disease alleles into large animal models, to evaluate potential genetic improvement of complex health and production traits in a single generation.
    Type of Medium: Online Resource
    ISSN: 1742-464X , 1742-4658
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2172518-4
    SSG: 12
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  • 2
    In: Animal Genetics, Wiley, Vol. 47, No. 5 ( 2016-10), p. 630-631
    Type of Medium: Online Resource
    ISSN: 0268-9146 , 1365-2052
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1472889-8
    SSG: 22
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Wiley ; 2022
    In:  International Journal of Applied Ceramic Technology Vol. 19, No. 3 ( 2022-05), p. 1727-1735
    In: International Journal of Applied Ceramic Technology, Wiley, Vol. 19, No. 3 ( 2022-05), p. 1727-1735
    Abstract: The stability of the grouting material under the coupling effect of sulfate attack and dry–wet cycles is essential to engineering safety. As a novel grouting material, the stability research of high‐water quick‐setting material (HWQSM) under different corrosive environments is in the initial stage. Moreover, the stratified damage characteristics (SDC) of HWQSM under sulfate attack were not fully explored. To this end, the strength degradation and SDC of HWQSM exposed to three kinds of sulfate solutions (i.e., 10% MgSO 4 solution, 10% Na 2 SO 4 solution, and hybrid solution of 5% Na 2 SO 4 and 5% MgSO 4 ) and dry–wet cycles were analyzed by using ETM‐C mechanical testing machine, scanning electron microscopy (SEM), XRD, and AFM. The results show that the strength reduction rate, the thickness of the corrosion layer, and roughness between the corrosion layer and the transition layer of HWQSM are all positively correlated with the concentration of Mg 2+ in the erosion solution for the increase in dry–wet cycles. Under the combined action of ion erosion and carbonization, the pores and cracks of the corrosion layer expand, which would reduce the permeability resistance but increase the erosion damage rate. This research furnishes new views into the SDC of HWQSM under sulfate attack and dry–wet cycles, which would deeper our understanding to this special material undoubtedly; the research results are expected to furnish theoretical guidance of HWQSM resistance to different sulfate solutions.
    Type of Medium: Online Resource
    ISSN: 1546-542X , 1744-7402
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2167226-X
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