In:
The Analyst, Royal Society of Chemistry (RSC)
Abstract:
Extracellular vesicles (EVs), as a type of subcellular structure, have been extensively researched for their potential in developing advanced diagnostic technologies for various diseases. However, the biomolecular and biophysical heterogeneity of EVs has restricted their application in clinical settings. In this article, we developed a size-exclusion chromatography-based technique for simultaneous EV size subtyping and protein profiling. By eluting fluorescent aptamer-treated patient plasma through a size-exclusion column, the mixture can be classified into 50 nm aptamer-bound EVs, 100 nm aptamer-bound EVs and free-floating aptamers, which could further enable the multiplex EV membrane proteins profiling by analyzing the fluorescence intensities of EV-bound aptamers. Using this technique, we successfully identified EV size subtypes for differentiating gastrointestinal cancers prognosis states. Overall, we developed a rapid, user-friendly and low-cost EV size subtyping and protein profiling technique, which holds great potentials for identifying crucial EV size subtypes for disease diagnosis in clinic.
Type of Medium:
Online Resource
ISSN:
0003-2654
,
1364-5528
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2023
detail.hit.zdb_id:
1472713-4
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