In:
PLOS Biology, Public Library of Science (PLoS), Vol. 20, No. 11 ( 2022-11-17), p. e3001868-
Abstract:
The striatum links neuronal circuits in the human brain, and its malfunction causes neuronal disorders such as Huntington’s disease (HD). A human striatum model that recapitulates fetal striatal development is vital to decoding the pathogenesis of striatum-related neurological disorders and developing therapeutic strategies. Here, we developed a method to construct human striatal organoids (hStrOs) from human pluripotent stem cells (hPSCs), including hStrOs-derived assembloids. Our hStrOs partially replicated the fetal striatum and formed striosome and matrix-like compartments in vitro. Single-cell RNA sequencing revealed distinct striatal lineages in hStrOs, diverging from dorsal forebrain fate. Using hStrOs-derived assembloids, we replicated the striatal targeting projections from different brain parts. Furthermore, hStrOs can serve as hosts for striatal neuronal allografts to test allograft neuronal survival and functional integration. Our hStrOs are suitable for studying striatal development and related disorders, characterizing the neural circuitry between different brain regions, and testing therapeutic strategies.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001868
DOI:
10.1371/journal.pbio.3001868.g001
DOI:
10.1371/journal.pbio.3001868.g002
DOI:
10.1371/journal.pbio.3001868.g003
DOI:
10.1371/journal.pbio.3001868.g004
DOI:
10.1371/journal.pbio.3001868.g005
DOI:
10.1371/journal.pbio.3001868.g006
DOI:
10.1371/journal.pbio.3001868.s001
DOI:
10.1371/journal.pbio.3001868.s002
DOI:
10.1371/journal.pbio.3001868.s003
DOI:
10.1371/journal.pbio.3001868.s004
DOI:
10.1371/journal.pbio.3001868.s005
DOI:
10.1371/journal.pbio.3001868.s006
DOI:
10.1371/journal.pbio.3001868.s007
DOI:
10.1371/journal.pbio.3001868.s008
DOI:
10.1371/journal.pbio.3001868.s009
DOI:
10.1371/journal.pbio.3001868.s010
DOI:
10.1371/journal.pbio.3001868.s011
DOI:
10.1371/journal.pbio.3001868.s012
DOI:
10.1371/journal.pbio.3001868.s013
DOI:
10.1371/journal.pbio.3001868.s014
DOI:
10.1371/journal.pbio.3001868.s015
DOI:
10.1371/journal.pbio.3001868.s016
DOI:
10.1371/journal.pbio.3001868.s017
DOI:
10.1371/journal.pbio.3001868.s018
DOI:
10.1371/journal.pbio.3001868.r001
DOI:
10.1371/journal.pbio.3001868.r002
DOI:
10.1371/journal.pbio.3001868.r003
DOI:
10.1371/journal.pbio.3001868.r004
DOI:
10.1371/journal.pbio.3001868.r005
DOI:
10.1371/journal.pbio.3001868.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2126773-X
Permalink