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  • Hindawi Limited  (2)
  • Chen, Xiao-Long  (2)
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  • Hindawi Limited  (2)
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  • 1
    Online Resource
    Online Resource
    Robot-Assisted versus Laparoscopic-Assisted Gastrectomy among Gastric Cancer Patients: A Retrospective Short-Term Analysis from a Single Institution in China
    Hindawi Limited ; 2019
    In:  Gastroenterology Research and Practice Vol. 2019 ( 2019-10-23), p. 1-9
    Details
    In: Gastroenterology Research and Practice, Hindawi Limited, Vol. 2019 ( 2019-10-23), p. 1-9
    Abstract: Background . The da Vinci robotic system was considered an effectively alternative treatment option for early gastric cancer patients in recent years. The aim of our study was to evaluate the safety and feasibility of robot-assisted gastrectomy in our center. Methods . This study included 33 patients who underwent robot-assisted gastrectomy (RAG) and 88 patients who underwent laparoscopic-assisted gastrectomy (LAG) between January 2016 and April 2018. Clinicopathological characteristics, surgical parameters, postoperative recovery, and the learning curves of RAG were evaluated. Results . Baseline characteristics between two groups were well balanced. The operation time of RAG was longer than that of LAG ( 333.1 ± 61.4   min vs. 290.6 ± 39.0   min , p = 0.001 ), and the estimated blood loss was 62.4 ± 41.2   ml in the RAG group and 77.7 ± 32.3   ml in the LAG group ( p = 0.005 ), respectively. The mean number of examined lymph nodes in RAG was less than that in LAG ( 30.3 ± 10.2 vs. 37.4 ± 13.7 , p = 0.008 ). However, RAG had an advantage in the dissection of No. 9 lymph nodes ( 3.4 ± 2.1 vs. 2.5 ± 1.6 , p = 0.039 ). The incidence of postoperative complications was similar in both groups ( p = 0.735 ). There were no significant differences in terms of postoperative recovery between the two groups. The learning curve of RAG showed that the CUSUM value decreased from the 8th case, which suggested a rapid learning curve among experienced surgeons on LAG operations. Conclusions . RAG was safe and feasible for gastric cancer patients, with superiority in the dissection of No. 9 lymph nodes.
    Type of Medium: Online Resource
    ISSN: 1687-6121 , 1687-630X
    URL: Article
    DOI: 10.1155/2019/9059176
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2435460-0
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Peritoneal Metastatic Cancer Stem Cells of Gastric Cancer with Partial Mesenchymal-Epithelial Transition and Enhanced Invasiveness in an Intraperitoneal Transplantation Model
    Hindawi Limited ; 2020
    In:  Gastroenterology Research and Practice Vol. 2020 ( 2020-08-05), p. 1-13
    Details
    In: Gastroenterology Research and Practice, Hindawi Limited, Vol. 2020 ( 2020-08-05), p. 1-13
    Abstract: Objectives . This preliminary study is aimed at enriching and isolating peritoneal metastatic cancer stem cells (pMCSCs) of gastric cancer and assessing their epithelial-mesenchymal transition (EMT) phenotype and invasiveness. Methods . Cancer stem cells of human gastric cancer (CSC-hGC) were previously isolated and transfected with green fluorescent protein and luciferase genes to validate the mouse model of peritoneal metastasis established via transplantation. The first and second generations ([G1] and [G2] , respectively) of pMCSCs were isolated from intraperitoneally transplanted CSC-hGC (pMCSC-tGC) by spherical culture. CSC and EMT-related markers and regulators in the two generations of intraperitoneally transplanted tumors were examined by immunohistochemistry, immunofluorescence staining, and quantitative PCR. Cell mobility was examined by a transwell assay. Results . The nude mouse model of intraperitoneally transplanted CSC-hGC was successful in establishing sequential formation of peritoneal tumors and enrichment of pMCSCs. CD44 and CD54 were consistently expressed in the two generations of transplanted tumors. In vitro cell (migration) assays and immunocytofluorescence assays showed that in pMCSC-tGC [G2] , E-cad, Survivin, and Vimentin expression was stable; α -SMA expression was decreased; and OVOL2, GRHL2, and ZEB1 expression was increased. PCR analysis indicated that in pMCSC-tGC [G2] , the mRNA expression of E-cad, α -SMA, MMP9, MMP2, and Vimentin was downregulated, while that of ZEB1, OVOL2, and GRHL2 was upregulated. In vivo tumor (homing) assays and immunohistochemical assays demonstrated that in pMCSC-tGC [G2] , E-cad and Snail were upregulated, while α -SMA was downregulated. The numbers of migrated and invaded pMCSC-tGC [G1] and pMCSC-tGC [G2] were significantly higher than those of CSC-hGC in migration and invasion assays. Conclusions . pMCSCs might be a specific subpopulation that can be sequentially enriched by intraperitoneal transplantation. pMCSCs exhibited a tendency towards partial mesenchymal-epithelial transition, enhancing their invasiveness during homing and the formation of peritoneal tumors. However, these preliminary findings require validation in further experiments.
    Type of Medium: Online Resource
    ISSN: 1687-6121 , 1687-630X
    URL: Article
    DOI: 10.1155/2020/3256538
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2435460-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
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