In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 54, No. 8 ( 2010-08), p. 3107-3112
Abstract:
The contribution of the bla OXA-58 gene and its promoter to β-lactam resistance has not been validated in Acinetobacter spp. other than Acinetobacter baumannii . We identified a multidrug-resistant (including carbapenem resistance) Acinetobacter genomic species 13TU in which bla OXA-58 was the only detected carbapenemase gene. The bla OXA-58 gene was plasmid located, flanked by IS Aba3 (downstream) and an IS Aba3- like element (upstream). An IS 1006 element was inserted into IS Aba3 -like (IS 1006 -ΔIS Aba3 -like) to generate a hybrid promoter for bla OXA-58 , with a −35 promoter located in IS 1006 and a −10 promoter in IS Aba3 -like. The reference strain of Acinetobacter genomic species 13TU, ATCC 17903, revealed higher MICs of amoxicillin, ticarcillin, and piperacillin and heteroresistance to imipenem and meropenem when it was transformed with a shuttle vector containing a fragment encompassing ΔIS Aba3 -like- bla OXA-58 , compared to the same host containing only bla OXA-58 . When the fragment was changed from ΔIS Aba3 -like- bla OXA-58 to IS 1006 -ΔIS Aba3 -like- bla OXA-58 , the ATCC 17903 transformant revealed a markedly higher level of bla OXA-58 transcription (12-fold), increased cefuroxime and piperacillin-tazobactam MICs, and homoresistance to imipenem and meropenem. Different roles of the insertion elements preceding the bla OXA-58 gene in Acinetobacter genomic species 13TU are demonstrated. The IS Aba3 -like- -bla OXA-58 construct can mediate resistance to penicillin derivatives but only heteroresistance to carbapenems. The insertion of IS 1006 into IS Aba3 -like, generating a hybrid promoter, could further enhance the transcription of bla OXA-58 and mediate homoresistance to carbapenems and also enhanced resistance to piperacillin-tazobactam.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.00128-10
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2010
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3
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