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Development and validation of a prognostic model incorporating tumor thrombus grading for nonmetastatic clear cell renal cell carcinoma with tumor thrombus: A multicohort study

Qu, Le ; Chen, Hui ; Chen, Qi ; [et al.]

Wiley ; 2023

In: MedComm Vol. 4, No. 4 ( 2023-08)

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In: MedComm, Wiley, Vol. 4, No. 4 ( 2023-08)

Abstract: There is significant variability with respect to the prognosis of nonmetastatic clear cell renal cell carcinoma (ccRCC) patients with venous tumor thrombus (VTT). By applying multiregion whole‐exome sequencing on normal‐tumor‐thrombus‐metastasis quadruples from 33 ccRCC patients, we showed that metastases were mainly seeded by VTT (81.8%) rather than primary tumors (PTs). A total of 706 nonmetastatic ccRCC patients with VTT from three independent cohorts were included in this study. C‐index analysis revealed that pathological grading of VTT outperformed other indicators in risk assessment (OS: 0.663 versus 0.501–0.610, 0.667 versus 0.544–0.651, and 0.719 versus 0.511–0.700 for Training, China‐Validation, and Poland‐Validation cohorts, respectively). We constructed a risk predicting model, TT‐GPS score, based on four independent variables: V TT height, VTT g rading, p erinephric fat invasion, and s arcomatoid differentiation in PT. The TT‐GPS score displayed better discriminatory ability (OS, c‐index: 0.706–0.840, AUC: 0.788–0.874; DFS, c‐index: 0.691–0.717, AUC: 0.771–0.789) than previously reported models in risk assessment. In conclusion, we identified for the first‐time pathological grading of VTT as an unheeded prognostic factor. By incorporating VTT grading, the TT‐GPS score is a promising prognostic tool in predicting the survival of nonmetastatic ccRCC patients with VTT.

Type of Medium: Online Resource

ISSN: 2688-2663 , 2688-2663

URL: Issue

URL: Article

DOI: 10.1002/mco2.v4.4

DOI: 10.1002/mco2.300

Language: English

Publisher: Wiley

Publication Date: 2023

detail.hit.zdb_id: 3021470-1

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A simple integrated system for rapid analysis of sialic‐acid‐containing N ‐glycopeptides from human serum

Zhu, Jun ; Wang, Fangjun ; Cheng, Kai ; [et al.]

Wiley ; 2013

In: PROTEOMICS Vol. 13, No. 8 ( 2013-04), p. 1306-1313

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In: PROTEOMICS, Wiley, Vol. 13, No. 8 ( 2013-04), p. 1306-1313

Abstract: Terminal sialylation is very important in cancer biology and has been extensively investigated for the discovery of potential clinical biomarkers of cancers. In this study, we presented a novel approach, by using of T i(IV)‐ IMAC , to enrich sialic‐acid‐containing N ‐glycopeptides for the analysis of terminal sialylation. Compared with conventional method using T i O 2 , this approach obtained 2.5 times more glycopeptides and glycosylation sites. Then, a simple integrated system combining filter‐aided sample preparation, ACN ‐improved digestion, and T i(IV)‐ IMAC enrichment was established for efficient analysis. In this system, protein digestion, glycopeptide enrichment, and deglycosylation were integrated and were performed sequentially in a single filter unit without any need for desalting, lyophilization, or sample transfer procedures. As a result, the number of identifications was improved by 1.5‐fold and the total processing time was drastically reduced to only 7–8 h. By using this system, fast and efficient analysis of human serum sialylated N ‐glycoproteome was achieved. From only 1 μL of human serum, 217 unique glycopeptides and 194 glycosylation sites were successfully identified.

Type of Medium: Online Resource

ISSN: 1615-9853 , 1615-9861

URL: Issue

URL: Article

DOI: 10.1002/pmic.v13.8

DOI: 10.1002/pmic.201200367

Language: English

Publisher: Wiley

Publication Date: 2013

detail.hit.zdb_id: 2037674-1

SSG: 12

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Murine Placental‐Fetal Phosphate Dyshomeostasis Caused by an Xpr1 Deficiency Accelerates Placental Calcification and Restricts Fetal Growth in Late Gestation

Xu, Xuan ; Li, Xiunan ; Sun, Hao ; [et al.]

Wiley ; 2020

In: Journal of Bone and Mineral Research Vol. 35, No. 1 ( 2020-01), p. 116-129

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In: Journal of Bone and Mineral Research, Wiley, Vol. 35, No. 1 ( 2020-01), p. 116-129

Abstract: Phosphorus is a necessary component of all living organisms. This nutrient is mainly transported from the maternal blood to the fetus via the placenta, and insufficient phosphorus availability via the placenta disturbs the normal development of the fetus, especially fetal bone formation in late gestation. Key proteins (phosphate transporters and exporters) that are responsible for the maintenance of placental‐fetal phosphorus homeostasis have been identified. A deficiency in the phosphate transporter Pit2 has been shown to result in placental calcification and the retardation of fetal development in mice. What roles does XPR1 (the only known phosphate exporter) play in maintaining placental‐fetal phosphorus homeostasis? In this study, we found that Xpr1 expression is strong in the murine placenta and increases with age during gestation. We generated a global Xpr1 knockout mouse and found that heterozygous ( Xpr1 +/− ) and homozygous ( Xpr1 −/− ) fetuses have lower inorganic phosphate (Pi) levels in amniotic fluid and serum and a decreased skeletal mineral content. Xpr1 ‐deficient placentas show abnormal Pi exchange during gestation. Therefore, Xpr1 deficiency in the placenta disrupts placental‐fetal Pi homeostasis. We also discovered that the placentas of the Xpr1 +/− and Xpr1 −/− embryos are severely calcified. Mendelian inheritance statistics for offspring outcomes indicated that Xpr1 ‐deficient embryos are significantly reduced in late gestation. In addition, Xpr1 −/− mice die perinatally and a small proportion of Xpr1 +/− mice die neonatally. RNA sequence (RNA‐Seq) analysis of placental mRNA revealed that many of the transcripts are significantly differentially expressed due to Xpr1 deficiency and are linked to dysfunction of the placenta. This study is the first to reveal that XPR1 plays an important role in maintaining placental‐fetal Pi homeostasis, disruption of which causes severe placental calcification, delays normal placental function, and restricts fetal growth. © 2019 American Society for Bone and Mineral Research.

Type of Medium: Online Resource

ISSN: 0884-0431 , 1523-4681

URL: Issue

URL: Article

DOI: 10.1002/jbmr.v35.1

DOI: 10.1002/jbmr.3866

RVK:

XA 52230

Language: English

Publisher: Wiley

Publication Date: 2020

detail.hit.zdb_id: 2008867-X

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Malonitrile‐Functionalized Tetraphenylpyrazine: Aggregation‐Induced Emission, Ratiometric Detection of Hydrogen Sulfide, and Mechanochromism

Chen, Ming ; Chen, Rui ; Shi, Yang ; [et al.]

Wiley ; 2018

In: Advanced Functional Materials Vol. 28, No. 6 ( 2018-02)

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In: Advanced Functional Materials, Wiley, Vol. 28, No. 6 ( 2018-02)

Abstract: Development of new aggregation‐induced emission (AIE) luminogens has been a hot research topic because they thoroughly solve the notorious aggregation‐caused quenching effect confronted in conventional fluorogens and their promising applications in, for example, organic light‐emitting diodes, chemo‐ and biosensors and bioimaging. Many AIE luminogens (AIEgens) have been prepared but most of them are silole, tetraphenylethene, distyrylanthracene, and their derivatives. In this work, based on the skeleton of tetraphenylpyrazine (TPP), a new AIEgen, named TPP‐PDCV, is generated by functionalizing TPP with malonitrile group. TPP‐PDCV can serve as a sensitive ratiometric fluorescent probe for detecting hydrogen sulfide with high speciality and low detection limit of down to 0.5 × 10 −6 m . The mechanism for such detection is fully investigated and deciphered. Unlike most reported mechanochromic AIEgens, which undergo turn‐off or ‐on emission or emission bathochromic shift in the presence of external stimuli, TPP‐PDCV exhibits an abnormal and reversible mechanochromism with hypsochromic effect. These indicate that TPP‐PDCV possesses a huge potential for high‐tech applications through rational modification of TPP core.

Type of Medium: Online Resource

ISSN: 1616-301X , 1616-3028

URL: Issue

URL: Article

DOI: 10.1002/adfm.v28.6

DOI: 10.1002/adfm.201704689

Language: English

Publisher: Wiley

Publication Date: 2018

detail.hit.zdb_id: 2029061-5

detail.hit.zdb_id: 2039420-2

SSG: 11

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A PRRT 2 variant in a Chinese family with paroxysmal kinesigenic dyskinesia and benign familial infantile seizures results in loss of interaction with STX 1B

Ma, Hongying ; Feng, Shenglei ; Deng, Xuejun ; [et al.]

Wiley ; 2018

In: Epilepsia Vol. 59, No. 8 ( 2018-08), p. 1621-1630

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In: Epilepsia, Wiley, Vol. 59, No. 8 ( 2018-08), p. 1621-1630

Abstract: To identify the causative gene of autosomal dominant paroxysmal kinesigenic dyskinesia and benign familial infantile seizures ( PKD / BFIS ) in a large Chinese family and explore the potential pathogenic mechanism of a PRRT 2 (proline‐rich transmembrane protein 2) variant. Methods Genetic testing was performed via whole exome sequencing. Western blotting and immunofluorescence were used to analyze the protein expression level and subcellular localization of the PRRT 2 mutant in HeLa cells and N2A cells. Coimmunoprecipitation was conducted to investigate the interaction of the PRRT 2 mutant with syntaxin 1B ( STX 1B). Results In a large Chinese family with autosomal dominant PKD / BFIS showing wide phenotypic heterogeneity, including patients suffering from PKD , BFIS , or epilepsy and asymptomatic variant carriers, a c.621dupA variant in PRRT 2 was identified in the proband and was shown to cosegregate with the phenotype in this family. This variant results in premature termination at codon 224, producing a truncated protein (p.Ser208Ilefs*17) in which the two conserved hydrophobic segments and the cytoplasmic loop are missing. Both the expression and subcellular localization of PRRT 2 are strongly affected by the c.621dupA variant. In addition, we found that PRRT 2 directly interacts with STX 1B, a SNARE protein critical for neurotransmitter release, whereas the truncated variant p.Ser208Ilefs*17 lacking the helix‐loop‐helix domain fails to bind to STX 1B. Significance Our findings identified a PRRT 2 variant in a family with PKD / BFIS and confirmed STX 1B as a new binding partner of PRRT 2, which suggested that the loss of the interaction between PRRT 2 and STX 1B may contribute to the pathogenesis of PKD / BFIS .

Type of Medium: Online Resource

ISSN: 0013-9580 , 1528-1167

URL: Issue

URL: Article

DOI: 10.1111/epi.2018.59.issue-8

DOI: 10.1111/epi.14511

RVK:

XA 10000

Language: English

Publisher: Wiley

Publication Date: 2018

detail.hit.zdb_id: 2002194-X

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A novel urinary long non‐coding RNA transcript improves diagnostic accuracy in patients undergoing prostate biopsy

Zhang, Wei ; Ren, Shan‐Cheng ; Shi, Xiao‐Lei ; [et al.]

Wiley ; 2015

In: The Prostate Vol. 75, No. 6 ( 2015-05), p. 653-661

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In: The Prostate, Wiley, Vol. 75, No. 6 ( 2015-05), p. 653-661

Abstract: Long non‐coding RNA (LncRNA) PCA3 has been a well‐established urine biomarker for the detection of prostate cancer (PCa). Our previous study showed a novel LncRNA FR0348383 is up‐regulated in over 70% of PCa compared with matched benign tissues. The aim of this study was to evaluate the diagnostic value of urinary FR0348383 for men undergoing prostate biopsy due to elevated PSA (PSA 〉 4.0 ng/ml) and/or abnormal digital rectal examination (DRE). Methods Post‐DRE first‐catch urine specimens prior to prostate biopsies were prospectively collected. After the whole transcriptome amplification, quantitative real time polymerase chain reaction was applied to quantify urine FR0348383 and PSA levels. The FR0348383 score was calculated as the ratio of PSA and FR0348383 mRNA (PSA mRNA/FR0348383 mRNA × 1000). The diagnostic value of FR0348383 score was evaluated by logistic regression and decision curve analysis. Results 213 cases with urine samples containing sufficient mRNA were included, 94 cases had serum PSA level 4.0–10.0 ng/ml. PCa was identified in 72 cases. An increasing FR0348383 score was correlated with an increasing probability of a positive biopsy ( P 〈 0.001). Multivariable logistic analysis indicated FR0348383 score ( P 〈 0.001), PSA ( P = 0.004), age ( P = 0.007), prostate volume ( P 〈 0.001) were independent predictors of PCa. ROC analysis demonstrated FR0348383 score outperformed PSA, %free PSA, and PSA Density in the prediction of PCa in the subgroup of patients with grey area PSA (AUC: 0.815 vs. 0.562 vs. 0.599 vs. 0.645). When using a probability threshold of 30% in the grey zone cohort, The FR0348383 score would save 52.0% of avoidable biopsies without missing any high grade cancers. Conclusions FR0348383 transcript in post‐DRE urine may be a novel biomarker for detection of PCa with great diagnostic value, especially in the grey zone cohort. The application of FR0348383 score in clinical practice might avoid unnecessary prostate biopsies and increase the specificity of PCa diagnosis. Prostate 75: 653–661, 2015 . © 2015 Wiley Periodicals, Inc.

Type of Medium: Online Resource

ISSN: 0270-4137 , 1097-0045

URL: Issue

URL: Article

DOI: 10.1002/pros.v75.6

DOI: 10.1002/pros.22949

Language: English

Publisher: Wiley

Publication Date: 2015

detail.hit.zdb_id: 1494709-2

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A new method for quantitative analysis of cell surface glycoproteome

Sun, Zhen ; Chen, Rui ; Cheng, Kai ; [et al.]

Wiley ; 2012

In: PROTEOMICS Vol. 12, No. 22 ( 2012-11), p. 3328-3337

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In: PROTEOMICS, Wiley, Vol. 12, No. 22 ( 2012-11), p. 3328-3337

Type of Medium: Online Resource

ISSN: 1615-9853

URL: Article

DOI: 10.1002/pmic.201200150

Language: English

Publisher: Wiley

Publication Date: 2012

detail.hit.zdb_id: 2037674-1

SSG: 12

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