In:
Chemistry – A European Journal, Wiley, Vol. 25, No. 21 ( 2019-04-11), p. 5463-5471
Abstract:
Metal–organic frameworks (MOFs) for enzyme immobilization have already shown superior tunable and designable characteristics, however, their diverse responsive properties have rarely been exploited. In this work we integrated a responsive MOF into a MOF–enzyme composite with the purpose of designing an “all‐in‐one” multifunctional composite with catalytic and luminescence functions incorporated into a single particle. As a proof‐of‐concept, glucose oxidase (GOx) was encapsulated in situ within an oxygen (O 2 )‐sensitive, noble‐metal‐free, luminescent Cu I triazolate framework (MAF‐2), denoted as GOx@MAF‐2. Owing to the rigid scaffold of MAF‐2 and confinement effect, the GOx@MAF‐2 composite showed significantly improved stability (shelf life of 60 days and heat resistance up to 80 °C) as well as good selectivity and recyclability. More importantly, owing to the O 2 sensitivity of MAF‐2, the GOx@MAF‐2 composite exhibited a rapid and reversible response towards dissolved O 2 , thereby allowing direct and ratiometric sensing of glucose without the need for chromogenic substrates, cascade enzymatic reactions, or electrode systems. High sensitivity with a detection limit of 1.4 μ m glucose was achieved, and the glucose levels in human sera were accurately determined. This strategy has led to a new application for MOFs that can be facilely extended to other MOF–enzyme composites due to the multifunctionality of MOFs.
Type of Medium:
Online Resource
ISSN:
0947-6539
,
1521-3765
DOI:
10.1002/chem.201806146
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
1478547-X
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