In:
Journal of Porphyrins and Phthalocyanines, World Scientific Pub Co Pte Ltd, Vol. 22, No. 09n10 ( 2018-09), p. 925-934
Abstract:
Antimicrobial photodynamic therapy (aPDT) is an effective mean for killing bacteria in this era of increasing multi-antibiotic resistance, and possesses a number of unique advantages. Much effort has been devoted to the development a key component of aPDT photosensitizers (PSs). We synthesized a series of PSs with different positive charges (ZnPc(Lys)[Formula: see text] , where [Formula: see text] 3, 5, 7, and studied their antibacterial activities and mechanisms against Escherichia coli (E. coli). Interestingly, the ZnPc(Lys)[Formula: see text] derivative showed stronger antibacterial effect (MIC = 25.3 [Formula: see text]M) than the other two PSs (MICs = 50.6 [Formula: see text] M), even though this PS did not have the highest uptake on bacteria among these PSs. It was ZnPc(Lys)[Formula: see text] that possessed the highest bacterial uptake. ZnPc(Lys)[Formula: see text] was found to have the highest monomeric fractions (62.0%) on bacteria surface than the other two PSs (37.9% for [Formula: see text] 3 and 33.9% [Formula: see text] = 7). These results clearly demonstrate that PS conformation on bacterial surface as a key parameter determining antibacterial efficacy of PSs. Other mechanistic aspects of photodynamic effects, including PS binding kinetics, bacterial surface hydrophobicity, zeta potential of bacteria, membrane permeability and bacterial signaling pathways were also studied.
Type of Medium:
Online Resource
ISSN:
1088-4246
,
1099-1409
DOI:
10.1142/S1088424618500888
Language:
English
Publisher:
World Scientific Pub Co Pte Ltd
Publication Date:
2018
Permalink