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  • Wiley  (2)
  • Chen, Hong  (2)
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  • Wiley  (2)
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  • 1
    In: Animal Science Journal, Wiley, Vol. 91, No. 1 ( 2020-01)
    Abstract: To determine the causative variations associated with two chicken comb phenotypes, pendulous comb (PC) or upright comb (UC), two pooled genomic DNA samples from PC and UC chickens were re‐sequenced by Next‐Generation Sequencer, and genome‐wide Single nucleotide polymorphisms (SNPs) were detected. Using three selective sweep approaches, F ST , θπ, and Tajima's D, with top 5% window values serving as the threshold, a total of 84 positively selective genes (PSGs) were identified. There were no SNPs in exons of the PSGs with significant differences in allele frequencies between the two comb phenotype groups. Then, 515 differentially expressed genes (DEGs) between the PC and UC were identified by RNA‐seq. Three genes including CD36 (CD36 molecule), ADAMTSL3 (ADAMTS‐like 3), and AOX1 (aldehyde oxidases 1) are overlapped between PSGs and DEGs. After genotyping seven candidate SNPs in the regulatory regions of the three overlapping genes in 120 chickens from two other breeds, two variants (rs14607046 and rs731818051) in the regulatory regions of AOX1 and ADAMTSL3 were found to have significant differences in allele frequency between the PC and UC, suggesting that the two variants may be causative mutations for PC. Overall, our study shed light on the genetic basis underlying the PC phenotype in chickens.
    Type of Medium: Online Resource
    ISSN: 1344-3941 , 1740-0929
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2095161-9
    Location Call Number Limitation Availability
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  • 2
    In: Animal Science Journal, Wiley, Vol. 91, No. 1 ( 2020-01)
    Abstract: This study investigated the effects of beak trimming on a local broiler breed. A total of 648 one‐day‐old birds were randomly allocated into three treatments: no trimming (NBT), infrared beak trimming (IRBT), and hot‐blade beak trimming (HBBT). The performance, beak length, behavior, carcass traits, organ percentages, and meat quality were inspected. The body weight (BW) from 7 to 35 days in the IRBT group was significantly lower than that in the NBT group, and the BW from 7 to 49 days was lower than that in the HBBT group ( p  〈  .05). Compared with untrimmed birds, birds in the IRBT group had lower average daily feed intake (ADFI) from 15 to 21 days, and higher heart percentage and L* value in the breast muscle. The upper beak length at 28 and 49 days of age were longest in untrimmed birds, intermediate in birds in the HBBT group, and shortest in the IRBT groups ( p   〈 .05). No evidence was found that HBBT caused changes in performance, behavior, carcass traits, organ percentages, and meat quality except for lower change in spleen percentage. Taken together, IRBT had more influence at inhibiting early BW, ADFI, and upper beak length than HBBT in the local broiler breed.
    Type of Medium: Online Resource
    ISSN: 1344-3941 , 1740-0929
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2095161-9
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
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