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  • Cerff, Martin  (5)
  • 2010-2014  (5)
  • 1
    Online Resource
    Online Resource
    Harvesting fresh water and marine algae by magnetic separation: Screening of separation parameters and high gradient magnetic filtration
    Elsevier BV ; 2012
    In:  Bioresource Technology Vol. 118 ( 2012-08), p. 289-295
    Details
    In: Bioresource Technology, Elsevier BV, Vol. 118 ( 2012-08), p. 289-295
    Type of Medium: Online Resource
    ISSN: 0960-8524
    URL: Article
    DOI: 10.1016/j.biortech.2012.05.020
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2012
    detail.hit.zdb_id: 1501389-3
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    In situ magnetic separation of antibody fragments from Escherichia coli in complex media
    Springer Science and Business Media LLC ; 2013
    In:  BMC Biotechnology Vol. 13, No. 1 ( 2013-12)
    Details
    In: BMC Biotechnology, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2013-12)
    Abstract: In situ magnetic separation (ISMS) has emerged as a powerful tool to overcome process constraints such as product degradation or inhibition of target production. In the present work, an integrated ISMS process was established for the production of his-tagged single chain fragment variable (scFv) D1.3 antibodies (“D1.3”) produced by E. coli in complex media. This study investigates the impact of ISMS on the overall product yield as well as its biocompatibility with the bioprocess when metal-chelate and triazine-functionalized magnetic beads were used. Results Both particle systems are well suited for separation of D1.3 during cultivation. While the triazine beads did not negatively impact the bioprocess, the application of metal-chelate particles caused leakage of divalent copper ions in the medium. After the ISMS step, elevated copper concentrations above 120 mg/L in the medium negatively influenced D1.3 production. Due to the stable nature of the model protein scFv D1.3 in the biosuspension, the application of ISMS could not increase the overall D1.3 yield as was shown by simulation and experiments. Conclusions We could demonstrate that triazine-functionalized beads are a suitable low-cost alternative to selectively adsorb D1.3 fragments, and measured maximum loads of 0.08 g D1.3 per g of beads. Although copper-loaded metal-chelate beads did adsorb his-tagged D1.3 well during cultivation, this particle system must be optimized by minimizing metal leakage from the beads in order to avoid negative inhibitory effects on growth of the microorganisms and target production. Hereby, other types of metal chelate complexes should be tested to demonstrate biocompatibility. Such optimized particle systems can be regarded as ISMS platform technology, especially for the production of antibodies and their fragments with low stability in the medium. The proposed model can be applied to design future ISMS experiments in order to maximize the overall product yield while the amount of particles being used is minimized as well as the number of required ISMS steps.
    Type of Medium: Online Resource
    ISSN: 1472-6750
    URL: Article
    DOI: 10.1186/1472-6750-13-44
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2013
    detail.hit.zdb_id: 2052746-9
    SSG: 12
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  • 3
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    Semi‐continuous in situ magnetic separation for enhanced extracellular protease production—modeling and experimental validation
    Wiley ; 2013
    In:  Biotechnology and Bioengineering Vol. 110, No. 8 ( 2013-08), p. 2161-2172
    Details
    In: Biotechnology and Bioengineering, Wiley, Vol. 110, No. 8 ( 2013-08), p. 2161-2172
    Abstract: In modern biotechnology proteases play a major role as detergent ingredients. Especially the production of extracellular protease by Bacillus species facilitates downstream processing because the protease can be directly harvested from the biosuspension. In situ magnetic separation (ISMS) constitutes an excellent adsorptive method for efficient extracellular protease removal during cultivation. In this work, the impact of semi‐continuous ISMS on the overall protease yield has been investigated. Results reveal significant removal of the protease from Bacillus licheniformis cultivations. Bacitracin‐functionalized magnetic particles were successfully applied, regenerated and reused up to 30 times. Immediate reproduction of the protease after ISMS proved the biocompatibility of this integrated approach. Six subsequent ISMS steps significantly increased the overall protease yield up to 98% because proteolytic degradation and potential inhibition of the protease in the medium could be minimized. Furthermore, integration of semi‐continuous ISMS increased the overall process efficiency due to reduction of the medium consumption. Process simulation revealed a deeper insight into protease production, and was used to optimize ISMS steps to obtain the maximum overall protease yield. Biotechnol. Bioeng. 2013; 110: 2161–2172. © 2013 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 0006-3592 , 1097-0290
    URL: Issue
    URL: Article
    DOI: 10.1002/bit.v110.8
    DOI: 10.1002/bit.24893
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 1480809-2
    detail.hit.zdb_id: 280318-5
    SSG: 12
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  • 4
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    Online Resource
    Enhancing the growth of Physcomitrella patens by combination of monochromatic red and blue light – a kinetic study
    Wiley ; 2012
    In:  Biotechnology Journal Vol. 7, No. 4 ( 2012-04), p. 527-526
    Details
    In: Biotechnology Journal, Wiley, Vol. 7, No. 4 ( 2012-04), p. 527-526
    Abstract: In the current work we demonstrate the relevance of monochromatic light conditions in moss plant cell culture. Light intensity and illumination wavelength are important cultivation parameters due to their impact on growth and chlorophyll formation kinetics of the moss Physcomitrella patens. This moss was chosen as a model organism due to its capability to produce complex recombinant pharmaceutical proteins. Filamentous moss cells were cultivated in mineral medium in shaking flasks. The flasks were illuminated by light emitting diodes (LED) providing nearly monochromatic red and blue light as well as white light as a reference. A maximum growth rate of 0.78 day (1 was achieved under additional CO 2 aeration and no growth inhibition was observed under high light illumination. The application of dual red and blue light is the most effective way to reach high growth and chlorophyll formation rates while minimizing energy consumption of the LEDs. These observations are discussed as effects of photo sensory pigments in the moss. The combination of monochromatic red and blue light should be considered when a large scale process is set up.
    Type of Medium: Online Resource
    ISSN: 1860-6768 , 1860-7314
    URL: Issue
    URL: Article
    DOI: 10.1002/biot.v7.4
    DOI: 10.1002/biot.201100044
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2214038-4
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  • 5
    Online Resource
    Online Resource
    Relationship between light intensity and morphology of the moss Physcomitrella patens in a draft tube photo bioreactor
    Elsevier BV ; 2012
    In:  Biochemical Engineering Journal Vol. 60 ( 2012-1), p. 119-126
    Details
    In: Biochemical Engineering Journal, Elsevier BV, Vol. 60 ( 2012-1), p. 119-126
    Type of Medium: Online Resource
    ISSN: 1369-703X
    URL: Article
    DOI: 10.1016/j.bej.2011.10.012
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2012
    detail.hit.zdb_id: 995646-3
    detail.hit.zdb_id: 2012139-8
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