In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 12 ( 2021-12-13)
Abstract:
Multiple genes in sugarcane control sucrose accumulation and the biosynthesis of cell wall components; however, it is unclear how these genes are expressed in its apical culms. To better understand this process, we sequenced mRNA from +1 stem internodes collected from four genotypes with different concentrations of soluble solids. Culms were collected at four different time points, ranging from six to 12-month-old plants. Here we show differentially expressed genes related to sucrose metabolism and cell wall biosynthesis, including genes encoding invertases, sucrose synthase and cellulose synthase. Our results showed increased expression of invertases in IN84-58, the genotype with lower sugar and higher fiber content, as well as delayed expression of secondary cell wall-related cellulose synthase for the other genotypes. Interestingly, genes involved with hormone metabolism were differentially expressed across time points in the three genotypes with higher soluble solids content. A similar result was observed for genes controlling maturation and transition to reproductive stages, possibly a result of selection against flowering in sugarcane breeding programs. These results indicate that carbon partitioning in apical culms of contrasting genotypes is mainly associated with differential cell wall biosynthesis, and may include early modifications for subsequent sucrose accumulation. Co-expression network analysis identified transcription factors related to growth and development, showing a probable time shift for carbon partitioning occurred in 10-month-old plants.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2021.736797
DOI:
10.3389/fpls.2021.736797.s001
DOI:
10.3389/fpls.2021.736797.s002
DOI:
10.3389/fpls.2021.736797.s003
DOI:
10.3389/fpls.2021.736797.s004
DOI:
10.3389/fpls.2021.736797.s005
DOI:
10.3389/fpls.2021.736797.s006
DOI:
10.3389/fpls.2021.736797.s007
DOI:
10.3389/fpls.2021.736797.s008
DOI:
10.3389/fpls.2021.736797.s009
DOI:
10.3389/fpls.2021.736797.s010
DOI:
10.3389/fpls.2021.736797.s011
DOI:
10.3389/fpls.2021.736797.s012
DOI:
10.3389/fpls.2021.736797.s013
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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