In:
United European Gastroenterology Journal, Wiley, Vol. 6, No. 5 ( 2018-06), p. 691-701
Kurzfassung:
Helicobacter pylori infection causes long‐term chronic active gastritis, a risk factor for the intestinal and diffuse forms of gastric cancer. Most gastric cancers develop in a stepwise progression from chronic active gastritis to precursor lesions of gastric cancer. The early detection of gastric cancer improves survival. Studies with recent evidence have proposed circulating‐microRNAs as biomarkers of cancer. Objective The purpose of this study was to explore the circulating‐microRNA profile from H. pylori infection to gastric adenocarcinoma. Methods One hundred and twenty‐three patients were enrolled and assigned to the discovery or the validation sets. In the discovery phase, circulating‐microRNAs were measured by dye‐based quantitative polymerase chain reaction and a selection of circulating‐microRNAs was validated by probe‐based quantitative polymerase chain reaction. A quality control protocol was used. Results One hundred and sixty‐seven circulating‐microRNAs were detected. Precursor lesions of gastric cancer and gastric cancer patients showed the downregulation of eight and five circulating‐microRNAs, respectively. We further validated the deregulation of miR‐196a‐5p in precursor lesions of gastric cancer and the deregulation of miR‐134‐5p, miR‐144‐3p and miR‐451a in gastric cancer. However, circulating‐microRNAs exhibited moderate diagnostic performance due to the overlap of circulating‐microRNA expression between non‐cancer and cancer patients. miR‐144‐3p/miR‐451a expression levels were correlated. Interestingly, these microRNAs are in 17q11.2, a site of rearrangements associated with gastric cancer. Conclusion Circulating‐microRNAs are deregulated in precancerous and gastric cancer patients but efforts are needed to improve their diagnostic accuracy.
Materialart:
Online-Ressource
ISSN:
2050-6406
,
2050-6414
DOI:
10.1177/2050640618759433
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2018
ZDB Id:
2728585-6
Permalink