In:
Molecular Ecology Resources, Wiley, Vol. 15, No. 6 ( 2015-11), p. 1421-1434
Abstract:
Twelve eulachon ( Thaleichthys pacificus , Osmeridae) populations ranging from Cook Inlet, Alaska and along the west coast of North America to the Columbia River were examined by restriction‐site‐associated DNA ( RAD ) sequencing to elucidate patterns of neutral and adaptive variation in this high geneflow species. A total of 4104 single‐nucleotide polymorphisms ( SNP s) were discovered across the genome, with 193 putatively adaptive SNP s as determined by F ST outlier tests. Estimates of population structure in eulachon with the putatively adaptive SNP s were similar, but provided greater resolution of stocks compared with a putatively neutral panel of 3911 SNP s or previous estimates with 14 microsatellites. A cline of increasing measures of genetic diversity from south to north was found in the adaptive panel, but not in the neutral markers ( SNP s or microsatellites). This may indicate divergent selective pressures in differing freshwater and marine environments between regional eulachon populations and that these adaptive diversity patterns not seen with neutral markers could be a consideration when determining genetic boundaries for conservation purposes. Estimates of effective population size ( N e ) were similar with the neutral SNP panel and microsatellites and may be utilized to monitor population status for eulachon where census sizes are difficult to obtain. Greater differentiation with the panel of putatively adaptive SNP s provided higher individual assignment accuracy compared to the neutral panel or microsatellites for stock identification purposes. This study presents the first SNP s that have been developed for eulachon, and analyses with these markers highlighted the importance of integrating genome‐wide neutral and adaptive genetic variation for the applications of conservation and management.
Type of Medium:
Online Resource
ISSN:
1755-098X
,
1755-0998
DOI:
10.1111/men.2015.15.issue-6
DOI:
10.1111/1755-0998.12400
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2406833-0
SSG:
12
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