In:
FEBS Letters, Wiley, Vol. 350, No. 2-3 ( 1994-08-22), p. 203-206
Abstract:
PDC‐109 (13 kDa) is the most abundant component, and the major heparin‐binding protein, of bovine ( Bos taurus ) seminal plasma. Here, we show that PDC‐109 contains a single O‐linked oligosaccharide (NeuNAcα(2–6)‐Galβ(1–3)‐GalNAc‐) attached to Thr 11 . Immunoquantitation of PDC‐109 indicates that its concentration in seminal plasma is 15–20 mg/ml. Though PDC‐109 is not present on epididymal sperm, ejaculated spermatozoa on average are coated with (9.5 ± 0.3) × 10 6 molecules of PDC‐109/cell. This value remained constant in swim‐up sperm and decreased to (7.7 ± 0.4) × 10 6 /spermatozoon after incubation for 24 h in capacitation medium at 39°C. These data substantiate the hypothesis that PDC‐109 may be one of the seminal plasma components that enhance the fertilizing capacity of bull spermatozoa upon interaction with heparin‐like glycosaminoglycans present in the female genital tract.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(94)00768-3
Language:
English
Publisher:
Wiley
Publication Date:
1994
detail.hit.zdb_id:
1460391-3
SSG:
12
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