In:
Environmental Microbiology, Wiley, Vol. 18, No. 6 ( 2016-06), p. 1686-1703
Abstract:
Research on geologic carbon sequestration raises questions about potential impacts of subsurface microbiota on carbon cycling and biogeochemistry. Subsurface, high‐ CO 2 systems are poorly biologically characterized, partly because of difficulty accessing high‐volume, uncontaminated samples. CO 2 ‐driven C rystal G eyser ( CG , U tah, USA ), an established geologic carbon sequestration analogue, provides high volumes of deep (∼ 200–500 m) subsurface fluids. We explored microbial diversity and metabolic potential in this high‐ CO 2 environment by assembly and analysis of metagenomes recovered from geyser water filtrate. The system is dominated by neutrophilic, iron‐oxidizing bacteria, including ‘marine’ M ariprofundus ( Z etaproteobacteria ) and ‘freshwater’ Gallionellales , sulfur‐oxidizing T hiomicrospira crunogena and T hiobacillus ‐like Hydrogenophilales . Near‐complete genomes were reconstructed for these bacteria. CG is notably populated by a wide diversity of bacteria and archaea from phyla lacking isolated representatives (candidate phyla) and from as‐yet undefined lineages. Many bacteria affiliate with OD 1, OP 3, OP 9, PER , ACD 58, WWE 3, BD 1‐5, OP 11, TM 7 and ZB 2. The recovery of nearly 100 genes encoding ribulose‐1,5 bisphosphate carboxylase‐oxygenase subunit proteins of the C alvin cycle and AMP salvage pathways suggests a strong biological role in high‐ CO 2 subsurface carbon cycling. Overall, we predict microbial impacts on subsurface biogeochemistry via iron, sulfur, and complex carbon oxidation, carbon and nitrogen fixation, fermentation, hydrogen metabolism, and aerobic and anaerobic respiration.
Type of Medium:
Online Resource
ISSN:
1462-2912
,
1462-2920
DOI:
10.1111/emi.2016.18.issue-6
DOI:
10.1111/1462-2920.12817
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2020213-1
SSG:
12
Permalink