In:
eLife, eLife Sciences Publications, Ltd, Vol. 8 ( 2019-06-17)
Abstract:
Functional tissue architecture originates by self-assembly of distinct cell types, following tissue-specific rules of cell-cell interactions. In the liver, a structural model of the lobule was pioneered by Elias in 1949. This model, however, is in contrast with the apparent random 3D arrangement of hepatocytes. Since then, no significant progress has been made to derive the organizing principles of liver tissue. To solve this outstanding problem, we computationally reconstructed 3D tissue geometry from microscopy images of mouse liver tissue and analyzed it applying soft-condensed-matter-physics concepts. Surprisingly, analysis of the spatial organization of cell polarity revealed that hepatocytes are not randomly oriented but follow a long-range liquid-crystal order. This does not depend exclusively on hepatocytes receiving instructive signals by endothelial cells, since silencing Integrin-β1 disrupted both liquid-crystal order and organization of the sinusoidal network. Our results suggest that bi-directional communication between hepatocytes and sinusoids underlies the self-organization of liver tissue.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.44860.001
DOI:
10.7554/eLife.44860.002
DOI:
10.7554/eLife.44860.003
DOI:
10.7554/eLife.44860.004
DOI:
10.7554/eLife.44860.005
DOI:
10.7554/eLife.44860.006
DOI:
10.7554/eLife.44860.007
DOI:
10.7554/eLife.44860.008
DOI:
10.7554/eLife.44860.009
DOI:
10.7554/eLife.44860.010
DOI:
10.7554/eLife.44860.011
DOI:
10.7554/eLife.44860.012
DOI:
10.7554/eLife.44860.013
DOI:
10.7554/eLife.44860.014
DOI:
10.7554/eLife.44860.015
DOI:
10.7554/eLife.44860.016
DOI:
10.7554/eLife.44860.017
DOI:
10.7554/eLife.44860.018
DOI:
10.7554/eLife.44860.019
DOI:
10.7554/eLife.44860.020
DOI:
10.7554/eLife.44860.021
DOI:
10.7554/eLife.44860.023
DOI:
10.7554/eLife.44860.024
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2019
detail.hit.zdb_id:
2687154-3
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