In:
BioTechniques, Future Science Ltd, Vol. 61, No. 1 ( 2016-07), p. 20-25
Abstract:
Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the environment. Here we present a comparative study of the performance of six fluorescent intercalating dyes—SYTO-9, SYTO-13, SYTO-82, SYBR Green I, SYBR Gold, EvaGreen—in three different qLAMP model systems. SYTO-9 and SYTO-82, which had the best results, were used for additional enzyme and template titration studies. SYTO-82 demonstrated the best combination of time-to-threshold (T t ) and signal-to-noise ratio (SNR).
Type of Medium:
Online Resource
ISSN:
0736-6205
,
1940-9818
Language:
English
Publisher:
Future Science Ltd
Publication Date:
2016
detail.hit.zdb_id:
1496354-1
SSG:
12
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