In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2019-01-18)
Kurzfassung:
By the time cardiotoxicity-associated cardiac dysfunction is detectable by echocardiography it is often beyond meaningful intervention. 99m Tc-sestamibi is used clinically to image cardiac perfusion by single photon emission computed tomography (SPECT) imaging, but as a lipophilic cation its distribution is also governed by mitochondrial membrane potential (ΔΨ m ). Correcting scans for variations in perfusion (using a ΔΨ m -independent perfusion tracer such as (bis(N-ethoxy-N-ethyldithiocarbamato)nitrido 99m Tc(V)) ( 99m Tc-NOET) could allow 99m Tc-sestamibi to be repurposed to specifically report on ΔΨ m as a readout of evolving cardiotoxicity. Isolated rat hearts were perfused within a γ-detection apparatus to characterize the pharmacokinetics of 99m Tc-sestamibi and 99m Tc-NOET in response to mitochondrial perturbation by hypoxia, ionophore (CCCP) or doxorubicin. All interventions induced 99m Tc-sestamibi washout; hypoxia from 24.9 ± 2.6% ID to 0.4 ± 6.2%, CCCP from 22.8 ± 2.5% ID to −3.5 ± 3.1%, and doxorubicin from 23.0 ± 2.2% ID to 17.8 ± 0.7, p 〈 0.05. Cardiac 99m Tc-NOET retention (34.0 ± 8.0% ID) was unaffected in all cases. Translating to an i n vivo rat model, 2 weeks after bolus doxorubicin injection, there was a dose-dependent loss of cardiac 99m Tc-sestamibi retention (from 2.3 ± 0.3 to 0.9 ± 0.2 ID/g with 10 mg/kg (p 〈 0.05)), while 99m Tc-NOET retention (0.93 ± 0.16 ID/g) was unaffected. 99m Tc-NOET therefore traps in myocardium independently of the mitochondrial perturbations that induce 99m Tc-sestamibi washout, demonstrating proof-of-concept for an imaging approach to detect evolving cardiotoxicity.
Materialart:
Online-Ressource
ISSN:
2045-2322
DOI:
10.1038/s41598-018-36721-5
Sprache:
Englisch
Verlag:
Springer Science and Business Media LLC
Publikationsdatum:
2019
ZDB Id:
2615211-3
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