In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 16, No. 7 ( 2022-7-5), p. e0010518-
Kurzfassung:
Leishmania parasites cause cutaneous leishmaniasis (CL), a disease characterized by disfiguring, ulcerative skin lesions. Both parasite and host gene expression following infection with various Leishmania species has been investigated in vitro, but global transcriptional analysis following L . major infection in vivo is lacking. Thus, we conducted a comprehensive transcriptomic profiling study combining bulk RNA sequencing (RNA-Seq) and single-cell RNA sequencing (scRNA-Seq) to identify global changes in gene expression in vivo following L . major infection. Bulk RNA-Seq analysis revealed that host immune response pathways like the antigen processing and presentation pathway were significantly enriched amongst differentially expressed genes (DEGs) upon infection, while ribosomal pathways were significantly downregulated in infected mice compared to naive controls. scRNA-Seq analyses revealed cellular heterogeneity including distinct resident and recruited cell types in the skin following murine L . major infection. Within the individual immune cell types, several DEGs indicative of many interferon induced GTPases and antigen presentation molecules were significantly enhanced in the infected ears including macrophages, resident macrophages, and inflammatory monocytes. Ingenuity Pathway Analysis of scRNA-Seq data indicated the antigen presentation pathway was increased with infection, while EIF2 signaling is the top downregulated pathway followed by eIF4/p70S6k and mTOR signaling in multiple cell types including macrophages, blood and lymphatic endothelial cells. Altogether, this transcriptomic profile highlights known recruitment of myeloid cells to lesions and recognizes a potential role for EIF2 signaling in murine L . major infection in vivo.
Materialart:
Online-Ressource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0010518
DOI:
10.1371/journal.pntd.0010518.g001
DOI:
10.1371/journal.pntd.0010518.g002
DOI:
10.1371/journal.pntd.0010518.g003
DOI:
10.1371/journal.pntd.0010518.g004
DOI:
10.1371/journal.pntd.0010518.g005
DOI:
10.1371/journal.pntd.0010518.g006
DOI:
10.1371/journal.pntd.0010518.g007
DOI:
10.1371/journal.pntd.0010518.g008
DOI:
10.1371/journal.pntd.0010518.g009
DOI:
10.1371/journal.pntd.0010518.t001
DOI:
10.1371/journal.pntd.0010518.t002
DOI:
10.1371/journal.pntd.0010518.t003
DOI:
10.1371/journal.pntd.0010518.t004
DOI:
10.1371/journal.pntd.0010518.t005
DOI:
10.1371/journal.pntd.0010518.t006
DOI:
10.1371/journal.pntd.0010518.t007
DOI:
10.1371/journal.pntd.0010518.s001
DOI:
10.1371/journal.pntd.0010518.s002
DOI:
10.1371/journal.pntd.0010518.s003
DOI:
10.1371/journal.pntd.0010518.s004
DOI:
10.1371/journal.pntd.0010518.s005
DOI:
10.1371/journal.pntd.0010518.s006
DOI:
10.1371/journal.pntd.0010518.s007
DOI:
10.1371/journal.pntd.0010518.s008
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2022
ZDB Id:
2429704-5
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