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  • 1
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    Primary bilateral corneal nerve sheath neoplasm in a dog
    Wiley ; 2017
    In:  Veterinary Ophthalmology Vol. 20, No. 4 ( 2017-07), p. 365-371
    Details
    In: Veterinary Ophthalmology, Wiley, Vol. 20, No. 4 ( 2017-07), p. 365-371
    Abstract: A 15‐year‐old, neutered male, Shih Tzu cross developed progressive corneal stromal thickening and vascularization of the right eye, and 5 months later, of the left eye. Both eyes became blind due to extensive corneal opacification and were enucleated. Light microscopic examination revealed a diffuse corneal infiltrate of neoplastic mesenchymal cells, and immunohistochemistry revealed diffuse cytoplasmic vimentin immunoreactivity and variable cytoplasmic and nuclear immunoreactivity for S100 in the neoplastic cells. Transmission electron microscopy revealed desmosomes between contiguous cells, thread‐like cytoplasmic processes coated with basement membrane, extracellular bundles of collagen, and axonal degeneration consistent with features of a nerve sheath neoplasm. This is the first report of primary, bilateral corneal nerve sheath sarcoma in a canine.
    Type of Medium: Online Resource
    ISSN: 1463-5216 , 1463-5224
    URL: Issue
    URL: Article
    DOI: 10.1111/vop.2017.20.issue-4
    DOI: 10.1111/vop.12398
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2011043-1
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  • 2
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    Relative quantification of white blood cell mitochondrial DNA and assessment of mitochondria by use of transmission electron microscopy in English Springer Spaniels with and without retinal dysplasia
    American Veterinary Medical Association (AVMA) ; 2010
    In:  American Journal of Veterinary Research Vol. 71, No. 4 ( 2010-04), p. 454-459
    Details
    In: American Journal of Veterinary Research, American Veterinary Medical Association (AVMA), Vol. 71, No. 4 ( 2010-04), p. 454-459
    Abstract: Objective —To compare relative amounts of WBC mitochondrial DNA (mtDNA; assessed via real-time PCR assay) and morphology of lymphocyte mitochondria (assessed via transmission electron microscopy [TEM]) in blood samples collected from English Springer Spaniels with and without retinal dysplasia. Animals —7 and 5 client-owned English Springer Spaniels (1 to 11 years old) with and without retinal dysplasia, respectively. Procedures —Blood samples were obtained from affected and unaffected dogs via venipuncture. Genomic DNA was extracted from WBCs of the 7 affected and 5 unaffected dogs, and relative quantification of the cytochrome c oxidase subunit 1 gene ( COX1 ) was determined via analysis of real-time PCR results. White blood cells from 3 affected and 4 unaffected dogs were embedded in epoxide resin for TEM; cross sections were examined for lymphocytes, which were measured. The mitochondria within lymphocytes were quantified, and the mitochondrial surface area per lymphocyte cross section was calculated. A masked technique was used to compare mitochondrial morphology between the 2 groups. Results —Compared with the smallest measured quantity of mtDNA among unaffected dogs, mtDNA amounts varied among unaffected (1.08- to 4.76-fold differences) and affected dogs (1- to 2.68-fold differences). Analysis of lymphocyte measurements and mitochondrial surface area, morphology, and quantity revealed no significant differences between affected and unaffected dogs. Conclusions and Clinical Relevance —No significant differences were detected in relative amounts of WBC mtDNA or the size, number, or morphology of lymphocyte mitochondria in English Springer Spaniels affected with retinal dysplasia, compared with results for unaffected control dogs.
    Type of Medium: Online Resource
    ISSN: 0002-9645
    URL: Article
    DOI: 10.2460/ajvr.71.4.454
    Language: Unknown
    Publisher: American Veterinary Medical Association (AVMA)
    Publication Date: 2010
    detail.hit.zdb_id: 2056942-7
    SSG: 22
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  • 3
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    Ocular Pathology
    Elsevier BV ; 2015
    In:  Veterinary Clinics of North America: Equine Practice Vol. 31, No. 2 ( 2015-08), p. 425-448
    Details
    In: Veterinary Clinics of North America: Equine Practice, Elsevier BV, Vol. 31, No. 2 ( 2015-08), p. 425-448
    Type of Medium: Online Resource
    ISSN: 0749-0739
    URL: Article
    DOI: 10.1016/j.cveq.2015.04.001
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2015
    SSG: 21,22
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  • 4
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    Effect of prolonged photoperiod on ocular tissues of domestic turkeys
    Wiley ; 2017
    In:  Veterinary Ophthalmology Vol. 20, No. 3 ( 2017-05), p. 232-241
    Details
    In: Veterinary Ophthalmology, Wiley, Vol. 20, No. 3 ( 2017-05), p. 232-241
    Abstract: The objective of this study is to investigate the structural and functional ocular changes that develop in turkeys exposed to a photoperiod of 23 h of light (23L) compared with a photoperiod of 14 h of light (14L). Procedures Ten‐day‐old Nicholas heavy strain poults were exposed to either a 14L or 23L photoperiod. Between 16 and 18 weeks of age, equal numbers of turkeys per treatment group underwent ophthalmic examination (biomicroscopy, indirect ophthalmoscopy) ( n = 14), refractometry ( n = 20), keratometry ( n = 20), tonometry ( n = 20), and full‐field electroretinography ( ERG ) ( n = 14). Postmortem analyses included orbital magnetic resonance imaging ( MRI ) ( n = 10) and light microscopy ( n = 24) at 18 weeks of age. Results Autorefraction revealed a median of −0.13 for sphere in both groups ( P = 0.69), which is approximately emmetropia. The radius of curvature of the cornea was significantly higher ( P = 0.0001) and the refractive power of the cornea was significantly lower ( P = 0.0001) in the 23L group. The astigmatic power was significantly greater in the 23L group ( P = 0.0001). Mean intraocular pressure did not differ between groups ( P = 0.085). Turkeys from the 23L group had significantly larger globes in nasotemporal ( P = 0.0007), dorsoventral ( P = 0.015), and anterioposterior ( P = 0.021) directions, and anterior chambers were more shallow ( P = 0.0002). ERG s revealed the 23L group to have lower a‐ and b‐wave amplitudes and significantly lower cone flicker amplitudes ( P = 0.0008). Light microscopic examination revealed 23L turkeys to have significantly decreased numbers of nuclei in the outer nuclear layer ( P = 0.0001) and inner nuclear layer ( P = 0.0186), and decreased choroidal thickness ( P = 0.0008). The prevalence of cataract in the 23L group was significantly higher ( P = 0.001). Conclusions Exposing turkeys to a prolonged photoperiod induces significant ocular disease.
    Type of Medium: Online Resource
    ISSN: 1463-5216 , 1463-5224
    URL: Issue
    URL: Article
    DOI: 10.1111/vop.2017.20.issue-3
    DOI: 10.1111/vop.12395
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2011043-1
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  • 5
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    Alterations in conjunctival bacteria and antimicrobial susceptibility during topical administration of ofloxacin after cataract surgery in dogs
    American Veterinary Medical Association (AVMA) ; 2017
    In:  American Journal of Veterinary Research Vol. 78, No. 2 ( 2017-02), p. 207-214
    Details
    In: American Journal of Veterinary Research, American Veterinary Medical Association (AVMA), Vol. 78, No. 2 ( 2017-02), p. 207-214
    Abstract: OBJECTIVE To evaluate changes in conjunctival bacteria and antimicrobial susceptibility of bacteria after cataract surgery in dogs. ANIMALS 16 client-owned dogs. PROCEDURES Samples for aerobic and anaerobic culture were obtained from the conjunctival fornices of both eyes of dogs 24 hours before (week 0) and 1, 3, and 6 weeks after cataract surgery. Topical administration of ofloxacin (every 6 hours) was initiated 12 hours before surgery and continued for 3 weeks. In vitro antimicrobial susceptibility was determined by Kirby-Bauer disk diffusion and a commercially available test for ofloxacin. RESULTS Frequency of positive culture results was significantly higher at week 6 than at weeks 0 and 1. Bacterial load was more likely to be moderate or high at weeks 3 and 6 than at weeks 0 and 1. The most frequently cultured organism was Staphylococcus pseudintermedius (21/78 [26.9%]), followed by coagulase-negative Staphylococcus spp (19/78 [24.4%]). Staphylococcus pseudintermedius was the organism most frequently cultured at weeks 0 (5/12), 1 (4/12), and 6 (8/19), whereas frequency of this organism was lowest at week 3 (1/20). In contrast, coagulase-negative Staphylococcus spp were the most frequently cultured organisms at week 3 (10/20). There was a significant increase in the proportion of organisms resistant to ofloxacin at week 3, compared with the proportion at week 0. CONCLUSIONS AND CLINICAL RELEVANCE The number of bacterial organisms increased and the population of conjunctival bacteria was altered and had a higher proportion resistant to ofloxacin during the 6 weeks after cataract surgery for dogs treated by use of this protocol.
    Type of Medium: Online Resource
    ISSN: 0002-9645
    URL: Article
    DOI: 10.2460/ajvr.78.2.207
    Language: Unknown
    Publisher: American Veterinary Medical Association (AVMA)
    Publication Date: 2017
    detail.hit.zdb_id: 2056942-7
    SSG: 22
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  • 6
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    A retrospective study of the clinical, histological, and immunohistochemical manifestations of 5 dogs originally diagnosed histologically as necrotizing scleritis : Canine Necrotizing Scleritis
    Wiley ; 2012
    In:  Veterinary Ophthalmology Vol. 15, No. 2 ( 2012-03), p. 102-109
    Details
    In: Veterinary Ophthalmology, Wiley, Vol. 15, No. 2 ( 2012-03), p. 102-109
    Type of Medium: Online Resource
    ISSN: 1463-5216
    URL: Issue
    URL: Article
    DOI: 10.1111/vop.2012.15.issue-2
    DOI: 10.1111/j.1463-5224.2011.00948.x
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2011043-1
    SSG: 22
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  • 7
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    Measurement of tear production and intraocular pressure in conscious captive European fallow deer ( DAMA dama )
    Wiley ; 2018
    In:  Veterinary Medicine and Science Vol. 4, No. 3 ( 2018-08), p. 227-236
    Details
    In: Veterinary Medicine and Science, Wiley, Vol. 4, No. 3 ( 2018-08), p. 227-236
    Abstract: Normal values for intraocular pressure (IOP) and tear production in conscious cervids have not been reported to date. Based on trends in zoological institutions to perform non‐anaesthetized health exams, it is applicable to establish normal values in conscious animals, as anaesthesia and sedation can alter these parameters. The goal of this study was to estimate intraocular pressures using rebound tonometry and measure tear production values in a group of healthy, conscious, European fallow deer utilizing chute restraint. Evaluation of these values with regards to instrumentation and restraint variables will be assessed. Complete ophthalmic examinations, including estimation of IOP with rebound tonometry and measurement of tear production with Schirmer tear tests (STT) were performed on nine conscious European fallow deer ( Dama dama ) restrained in a chute. Correlations between IOP on the unspecified (P) and the equine (H) settings, as well as IOP and STT differences between left (OS) and right (OD) eyes were evaluated, in addition to assessment of correlations between right and left lateral recumbency on IOP and STT. Tear production measurements were 18.7 ± 5.1 mm min −1 with a 95% confidence interval (CI) range of 16.4–21.1 mm min −1 . Intraocular pressure measurements for the P setting were 16.1 ± 4.5 mmHg with a 95% CI range of 14.1–18.2 mmHg, and for the H setting were 21.5 ± 5.1 mmHg with a 95% CI range of 19.1–23.9 mmHg. No statistically significant difference ( P  〉   0.05) was found between OS and OD in any test. Neither left nor right lateral recumbency was found to have a statistically significant effect on IOP or STT. This study represents the first assessment of ophthalmic parameters in conscious fallow deer with rebound tonometry and STT.
    Type of Medium: Online Resource
    ISSN: 2053-1095 , 2053-1095
    URL: Issue
    URL: Article
    DOI: 10.1002/vms3.2018.4.issue-3
    DOI: 10.1002/vms3.105
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2819409-3
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  • 8
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    Immunohistochemical evaluation of fibrovascular and cellular pre‐iridal membranes in dogs
    Wiley ; 2012
    In:  Veterinary Ophthalmology Vol. 15, No. s1 ( 2012-03), p. 54-59
    Details
    In: Veterinary Ophthalmology, Wiley, Vol. 15, No. s1 ( 2012-03), p. 54-59
    Abstract: Objective  Histologically, two morphologically distinct types of pre‐iridal membranes appear to occur in diseased canine globes: fibrovascular and cellular. Cellular pre‐iridal membranes of corneal endothelial origin exist in iridocorneal endothelial (ICE) syndrome in humans and arise through metaplastic transformation of corneal endothelial cells into epithelial‐like cells. 1 The purpose of this study was to (i) evaluate immunohistochemical staining of these two types of membranes in diseased canine globes, (ii) determine whether endothelial cell metaplasia or iridal vascular budding plays a role in cellular membrane formation and (iii) compare the primary histopathologic diagnosis between the two groups. Procedures  Hematoxylin and eosin (H & E)‐stained slides of 28 enucleated canine specimens with pre‐iridal membranes were randomly selected and examined with light microscopy. The globes were divided into two groups based on the appearance of the membrane: fibrovascular or cellular, and the histopathologic diagnoses were recorded. Immunohistochemical staining for vimentin, cytokeratin AE1/AE3, and Von Willebrand’s factor (Factor VIII) was completed on the slides of each globe. The histopathologic diagnoses were compared between the two groups. Results  The fibrovascular and cellular membranes stained positive for vimentin and negative for cytokeratin AE1/AE3. All fibrovascular membranes stained positive for Factor VIII compared with the cellular membranes which stained negative. In the cellular membrane group, primary glaucoma was a common histologic diagnosis. Conclusions  Immunohistochemical evaluation in this study does not support the hypothesis of metaplastic transformation of endothelial cells into epithelial‐like cells in the canine globes with cellular membranes. The cellular membranes in this study do not represent a canine version of ICE syndrome and are not of vascular endothelial origin.
    Type of Medium: Online Resource
    ISSN: 1463-5216 , 1463-5224
    URL: Issue
    URL: Article
    DOI: 10.1111/vop.2012.15.issue-s1
    DOI: 10.1111/j.1463-5224.2011.00949.x
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2011043-1
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  • 9
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    Golden retriever cystic uveal disease: a longitudinal study of iridociliary cysts, pigmentary uveitis, and pigmentary/cystic glaucoma over a decade in western Canada
    Wiley ; 2016
    In:  Veterinary Ophthalmology Vol. 19, No. 3 ( 2016-05), p. 237-244
    Details
    In: Veterinary Ophthalmology, Wiley, Vol. 19, No. 3 ( 2016-05), p. 237-244
    Abstract: To determine the incidence of iridociliary cysts, pigmentary uveitis ( PU )/pigmentary cystic glaucoma ( PCG ) in golden retriever dogs in western Canada, the progression of iridociliary cysts to PU / PCG , and a mode of inheritance for this disorder. Animal studied A total of 830 golden retriever dogs from Alberta, Saskatchewan, and Manitoba from 2004 to 2014 were studied. Procedure Data were compiled from Canine Eye Registry Foundation ( CERF ) or Orthopedic Foundation for Animals ( OFA ) records ( n  =   630) and clinical consultations ( n  =   200) for a retrospective assessment of iridociliary cysts, PU , and PCG . Results Total incidence of iridociliary cysts and PU from CERF / OFA data were 4.8% ( n  =   30/630) and 5.9% ( n  =   37/630), respectively. Incidence of PU increased with ages 〉 4 years (12.7%, n  =   32/251). Dogs diagnosed with thin‐walled, attached iridociliary cysts had a high risk of being diagnosed with PU or PCG upon re‐examination (56.5%, n  =   13/23). No dogs diagnosed with thick‐walled, anterior chamber cysts ( n  =   7) developed PU or PCG within the time frame of the study. Data from clinical consultations confirmed that PU carried a poor prognosis for the affected eyes as 44.9% ( n  =   22/49) of dogs progressed to PCG . PU ‐ and PCG ‐affected dogs followed a familial pattern and there was an association with thin‐walled iridociliary cysts. Pedigree analysis suggested an autosomal dominant mode of inheritance with partial penetrance. Conclusions Thin‐walled iridociliary cysts are associated with PU and PCG . All breeding golden retriever dogs should be examined annually by an ophthalmologist. The incidence of this disorder is higher in western Canada than previous reports in North America.
    Type of Medium: Online Resource
    ISSN: 1463-5216 , 1463-5224
    URL: Issue
    URL: Article
    DOI: 10.1111/vop.2016.19.issue-3
    DOI: 10.1111/vop.12293
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2011043-1
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  • 10
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    Odontogenic parakeratinized cyst resulting in exophthalmos and palatine, maxillary, and zygomatic bone erosion in a dog
    Wiley ; 2018
    In:  Veterinary Ophthalmology Vol. 21, No. 5 ( 2018-09), p. 539-543
    Details
    In: Veterinary Ophthalmology, Wiley, Vol. 21, No. 5 ( 2018-09), p. 539-543
    Abstract: A novel case of a canine odontogenic parakeratinized cyst ( COPC ) that resulted in exophthalmos and palatine, maxillary, and zygomatic bone erosion in a 5‐year‐old Chihuahua dog is reported. Final diagnosis was aided by cross‐sectional imaging (magnetic resonance imaging and computed tomography) and confirmed with histologic examination of the cyst wall.
    Type of Medium: Online Resource
    ISSN: 1463-5216 , 1463-5224
    URL: Issue
    URL: Article
    DOI: 10.1111/vop.2018.21.issue-5
    DOI: 10.1111/vop.12538
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2011043-1
    SSG: 22
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