In:
Immunology & Cell Biology, Wiley, Vol. 100, No. 8 ( 2022-09), p. 636-652
Abstract:
Special AT‐binding protein 1 (SATB1) is a chromatin‐binding protein that has been shown to be a key regulator of T‐cell development and CD4 + T‐cell fate decisions and function. The underlying function for SATB1 in peripheral CD8 + T‐cell differentiation processes is largely unknown. To address this, we examined SATB1‐binding patterns in naïve and effector CD8 + T cells demonstrating that SATB1 binds to noncoding regulatory elements linked to T‐cell lineage–specific gene programs, particularly in naïve CD8 + T cells. We then assessed SATB1 function using N ‐ethyl‐ N ‐nitrosourea‐mutant mice that exhibit a point mutation in the SATB1 DNA‐binding domain (termed Satb1 m1Anu/m1Anu ). Satb1 m1Anu/m1Anu mice exhibit diminished SATB1‐binding, naïve, Satb1 m1Anu/m1Anu CD8 + T cells exhibiting transcriptional and phenotypic characteristics reminiscent of effector T cells. Upon activation, the transcriptional signatures of Satb1 m1Anu/m1Anu and wild‐type effector CD8 + T cells converged. While there were no overt differences, primary respiratory infection of Satb1 m1Anu/m1Anu mice with influenza A virus (IAV) resulted in a decreased proportion and number of IAV‐specific CD8 + effector T cells recruited to the infected lung when compared with wild‐type mice. Together, these data suggest that SATB1 has a major role in an appropriate transcriptional state within naïve CD8 + T cells and ensures appropriate CD8 + T‐cell effector gene expression upon activation.
Type of Medium:
Online Resource
ISSN:
0818-9641
,
1440-1711
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
2011707-3
SSG:
12
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