In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 152, No. 1 ( 2010), p. 32-40
Abstract:
〈 i 〉 Background: 〈 /i 〉 A disintegrin and metalloprotein-33 〈 i 〉 (ADAM33) 〈 /i 〉 participates in the bronchial remodeling process in asthma, and genetic analyses pointed it out as a candidate gene in asthma. 〈 i 〉 Methods: 〈 /i 〉 To analyze the association between 〈 i 〉 ADAM33 〈 /i 〉 and asthma and total and mite-specific IgE levels in a population of the Caribbean Coast of Colombia, we genotyped 6 single-nucleotide polymorphisms of 〈 i 〉 ADAM33 〈 /i 〉 in 429 asthmatics, 401 controls and 116 family trios using fluorogenic probes. Total and specific IgE against 〈 i 〉 Blomia tropicalis 〈 /i 〉 and 〈 i 〉 Dermatophagoides pteronyssinus 〈 /i 〉 were determined by ELISA. Case-control and family-based analyses were performed. Case-control association analyses were corrected by population stratification using a set of 52 ancestry-informative markers. 〈 i 〉 Results: 〈 /i 〉 Eight common haplotypes were identified; among them, H4 (GCAGGG) was associated with asthma in the family group (Z score: –2.049, p = 0.04). We also found an association between the TT genotype of ST+7 and asthma in the case-control study (p = 0.05) that disappeared after correcting for multiple testing. In the family-based analysis, this genotype was a risk factor for asthma (p = 0.01), high total IgE (Z score: 2.546, p = 0.01) and high specific IgE against 〈 i 〉 B. tropicalis 〈 /i 〉 (p = 0.02) and 〈 i 〉 D. pteronyssinus 〈 /i 〉 (Z score: 2.414, p = 0.01). V4 was associated with specific IgE against 〈 i 〉 B. tropicalis 〈 /i 〉 (p = 0.03); T2 with asthma (p = 0.03), high total IgE (p = 0.02) and IgE against D. 〈 i 〉 pteronyssinus 〈 /i 〉 (p = 0.03) and T1 with high total IgE (p = 0.04). None of these associations was maintained after correction for multiple testing. 〈 i 〉 Conclusions: 〈 /i 〉 Our findings suggest a relevant role of 〈 i 〉 ADAM33 〈 /i 〉 in thepathogenesis of asthma in this population.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
2010
detail.hit.zdb_id:
1482722-0
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