In:
Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 60, No. 12 ( 2014-12), p. 865-868
Abstract:
In our previous study, γ-glutamyl transpeptidase (GGT) isolated from Helicobacter pylori induced apoptosis of AGS cells. Here, we investigate Ca 2+ effects on GGT-induced apoptosis. The GGT transiently and significantly increased intracellular Ca 2+ concentration ([Ca 2+ ] i ) in AGS cells in a dose-dependent manner (P 〈 0.05). The GGT-induced Ca 2+ increase resulted from Ca 2+ influx and release through the phospholipase C – inositol 1,4,5-trisphosphate (PLC–IP3) pathway. The GGT-induced apoptosis was significantly reduced by treatment with U73122 (a PLC inhibitor) and xestospongin (an IP3 receptor antagonist) (P 〈 0.05). These results indicate that GGT could induce apoptosis of AGS cells by high levels of [Ca 2+ ] i .
Type of Medium:
Online Resource
ISSN:
0008-4166
,
1480-3275
DOI:
10.1139/cjm-2014-0464
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
2014
detail.hit.zdb_id:
280534-0
detail.hit.zdb_id:
1481972-7
SSG:
12
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