In:
Experimental Dermatology, Wiley, Vol. 21, No. 9 ( 2012-09), p. 716-718
Abstract:
Leucine‐rich glioma inactivated 3 ( LGI 3) is known to be expressed mainly in the brain. However, the expression and physiological roles of LGI 3 in skin cells remain unknown. In this study, it was found for the first time that LGI 3 is expressed mostly by normal human keratinocytes. Furthermore, ELISA analysis showed that H a C a T human keratinocytes increased LGI 3 secretion after exposure to ultraviolet B ( UVB ) in a time‐ and dose‐dependent manner. We next investigated the possible role of LGI 3 in keratinocytes. LGI 3 (50 ng/ml) increased survival of H a C a T cells by 20% after UVB irradiation (150 mJ/cm 2 ). It was also found that LGI 3 stimulates the phosphorylation of A kt, which is involved in the cell survival‐signalling cascade. Furthermore, LGI 3 led to the phosphorylation of MDM 2 and subsequent p53 degradation. Taken together, the data suggest that LGI 3 may regulate p53 levels and that keratinocyte‐derived LGI 3 may act as a novel cytokine for skin homoeostasis.
Type of Medium:
Online Resource
ISSN:
0906-6705
,
1600-0625
DOI:
10.1111/exd.2012.21.issue-9
DOI:
10.1111/j.1600-0625.2012.01550.x
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
2026228-0
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