In:
Journal of Separation Science, Wiley, Vol. 36, No. 12 ( 2013-06), p. 1904-1912
Abstract:
We developed and validated an accurate and sensitive LC – MS / MS method for the simultaneous quantitation of ginsenoside R g3 and R h2 epimers ( R ‐ R g3, S ‐ R g3, R ‐ R h2, and S ‐ R h2) in rat plasma. Analytes were extracted from 0.1 mL aliquots of rat plasma by liquid–liquid extraction, using 2 mL of ethyl acetate. In this assay, dioscin (500 ng/mL) was used as an internal standard. Chromatographic separation was conducted using an Acclaim RSLC C 18 column (150 × 2.1 mm, 2.2 μm) at 40°C, with a gradient mobile phase consisting of 0.1% formic acid in distilled water and in acetonitrile, a flow rate of 0.35 mL/min, and a total run time of 20 min. Detection and quantification were performed using a mass spectrometer in selected reaction‐monitoring mode with negative electrospray ionization at m/z 783.4 → 161.1 for R ‐ R g3 and S ‐ R g3, m/z 621.3 → 161.1 for R ‐ R h2 and S ‐ R h2, and m/z 867.2 → 761.5 for the internal standard. For R ‐ R g3 and S ‐ R g3, the lower limit of quantification was 5 ng/mL, with a linear range up to 500 ng/mL; for R ‐ R h2 and S ‐ R h2, the lower limit of quantification was 150 ng/mL, with a linear range up to 6000 ng/mL. The coefficient of variation for assay precision was less than 10.5%, with an accuracy of 86.4–112%. No relevant cross‐talk or matrix effect was observed. The method was successfully applied to a pharmacokinetic study after oral administration of 400 mg/kg and 2000 mg/kg of BST 204, a fermented ginseng extract, to rats. We found that the S epimers exhibited significantly higher plasma concentrations and area under curve values for both R g3 and R h2. This is the first report on the separation and simultaneous quantification of R ‐ R g3, S ‐ R g3, R ‐ R h2, and S ‐ R h2 in rat plasma by LC ‐ MS / MS . The method should be useful in the clinical use of ginseng or its derivatives.
Type of Medium:
Online Resource
ISSN:
1615-9306
,
1615-9314
DOI:
10.1002/jssc.201300107
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2047990-6
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