In:
Asian Spine Journal, Asian Spine Journal (ASJ), Vol. 11, No. 3 ( 2017-06-30), p. 329-336
Abstract:
〈 sec 〉 〈 title 〉 Study Design 〈 /title 〉 〈 p 〉 Controlled laboratory study. 〈 /p 〉 〈 /sec 〉 〈 sec 〉 〈 title 〉 Purpose 〈 /title 〉 〈 p 〉 This study aimed to evaluate the efficacy of platelet-rich plasma (PRP) stored at room temperature (RT), frozen, or after freeze-drying. 〈 /p 〉 〈 /sec 〉 〈 sec 〉 〈 title 〉 Overview of Literature 〈 /title 〉 〈 p 〉 PRP enriches tissue repair and regeneration, and is a novel treatment option for musculoskeletal pathologies. However, whether biological activity is preserved during PRP storage remains uncertain. 〈 /p 〉 〈 /sec 〉 〈 sec 〉 〈 title 〉 Methods 〈 /title 〉 〈 p 〉 PRP was prepared from blood of 12 healthy human volunteers (200 mL/person) and stored using three methods: PRP was stored at RT with shaking, PRP was frozen and stored at −80℃, or PRP was freeze-dried and stored at RT. Platelet counts and growth factor content were examined immediately after preparation, as well as 2, 4, and 8 weeks after storage. Platelet activation rate was quantified by flow cytometry. 〈 /p 〉 〈 /sec 〉 〈 sec 〉 〈 title 〉 Results 〈 /title 〉 〈 p 〉 Platelet counts were impossible to determine in many RT samples after 2 weeks, but they remained at constant levels in frozen and freeze-dried samples, even after 8 weeks of storage. Flow cytometry showed approximately 80% activation of the platelets regardless of storage conditions. Almost no growth factors were detected in the RT samples after 8 weeks, while low but significant expression was detected in the frozen and freeze-dried PRP. Over time, the mean relative concentrations of various growth factors decreased significantly or disappeared in the RT group. In the frozen group, levels were maintained for 4 weeks, but decreased significantly by 8 weeks ( 〈 italic 〉 p 〈 /italic 〉 & lt;0.05). The freeze-dried group maintained baseline levels of growth factors for the entire 8-week duration. 〈 /p 〉 〈 /sec 〉 〈 sec 〉 〈 title 〉 Conclusions 〈 /title 〉 〈 p 〉 Freeze-drying enables PRP storage while maintaining bioactivity and efficacy for extended periods. 〈 /p 〉 〈 /sec 〉
Type of Medium:
Online Resource
ISSN:
1976-1902
,
1976-7846
DOI:
10.4184/asj.2017.11.3.329
Language:
English
Publisher:
Asian Spine Journal (ASJ)
Publication Date:
2017
detail.hit.zdb_id:
2559763-2
detail.hit.zdb_id:
2502303-2
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