In:
Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 59, No. 2 ( 2021-03-16), p. 158-168
Abstract:
Triterpenoids are considered to be the major pharmaceutical active compounds found in the macrofungus Ganoderma lucidum (G. lucidum). Extraction conditions of triterpenoids from the Vietnamese red G. lucidum were optimized by response surface methodology using ultrasound –assisted extraction (UAE) method. A Box – Behnken experimental design was used to determine the simultaneous influences of three independent variables, namely solvent-to-material ratio, extraction time, and ultrasonic power on the yield of triterpenoids. The highest yield of triterpenoids obtained under optimum conditions including extraction time of 55 min, ultrasonic power of 480 W, solvent-to-material ratio of 27:1 mL/g was 9.5768 ± 0.228 mg/g according to ultraviolet-visible spectroscopy (UV-Vis) analysis. Besides, the hot water extraction method was carried out in order to compare with UAE in terms of the yield of triterpenoids. The results of anticancer evaluation of the extract obtained indicated that with the half-maximal inhibitory concentration value of 67.25 ± 0.82 µg/mL on the human Hep-G2 liver cancer cell lines, triterpenoids extracted from G. lucidum could be regarded as a potential agent for medicinal treatment.
Type of Medium:
Online Resource
ISSN:
2525-2518
,
2525-2518
DOI:
10.15625/2525-2518/59/2/15485
Language:
Unknown
Publisher:
Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
Publication Date:
2021
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