In:
eLife, eLife Sciences Publications, Ltd, Vol. 12 ( 2023-08-08)
Abstract:
Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient and inducible suppression of any gene upon introduction of a synthetic target sequence into non-coding transcript regions. ARTi establishes a scalable loss-of-function tool with full control over on- and off-target effects.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.84792.3
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2023
detail.hit.zdb_id:
2687154-3
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