In:
Journal of Pharmacy and Pharmacology, Oxford University Press (OUP), Vol. 71, No. 9 ( 2019-08-01), p. 1429-1439
Abstract:
To explore the effect of cordycepin on reducing lipid droplets in adipocytes. Methods Rats were fed a 60% high-fat diet to construct a hyperlipidaemia animal model and then treated with cordycepin at different concentrations for 8 weeks. Adipocytes were extracted, and BODIPY staining was used to detect the size of the lipid droplets. The adipocyte membrane proteins ASC-1, PAT2 and P2RX5 were assessed to determine the transformation of white adipocytes to beige and brown adipocytes. In an in vitro study, 3T3-L1 cells were cultured, and Western blotting was used to determine the expression of the lipid droplet-related genes Fsp27, perilipin 3, perilipin 2, PPAR-γ, Rab5, Rab7, Rab11, perilipin 1, ATGL and CGI-58. Results We found that cordycepin could promote the transformation of white adipocytes into beige and brown adipocytes. Cordycepin also downregulated the lipid droplet-associated genes Fsp27, perilipin 3, perilipin 2, Rab5, Rab11 and perilipin 1. Moreover, cordycepin reduced the expression of protein CGI-58, which inhibits lipid droplet degradation. In addition, cordycepin significantly increased the expression of ATGL, suggesting that cordycepin might stimulate lipolysis by upregulating the expression of ATGL instead of CGI-58 and by downregulating the expression of perilipin 1. Conclusions Cordycepin could blockade lipid droplet formation and promote lipid droplet degradation.
Type of Medium:
Online Resource
ISSN:
2042-7158
,
0022-3573
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2019
detail.hit.zdb_id:
2041988-0
detail.hit.zdb_id:
2050532-2
SSG:
15,3
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