In:
Journal of Cell Biology, Rockefeller University Press, Vol. 202, No. 7 ( 2013-09-30), p. 1057-1073
Kurzfassung:
A ctin filaments, with the aid of multiple accessory proteins, self-assemble into a variety of network patterns. We studied the organization and dynamics of the actin network in nonadhesive regions of cells bridging fibronectin-coated adhesive strips. The network was formed by actin nodes associated with and linked by myosin II and containing the formin disheveled-associated activator of morphogenesis 1 (DAAM1) and the cross-linker filamin A (FlnA). After Latrunculin A (LatA) addition, actin nodes appeared to be more prominent and demonstrated drift-diffusion motion. Superresolution microscopy revealed that, in untreated cells, DAAM1 formed patches with a similar spatial arrangement to the actin nodes. Node movement (diffusion coefficient and velocity) in LatA-treated cells was dependent on the level and activity of myosin IIA, DAAM1, and FlnA. Based on our results, we developed a computational model of the dynamic formin-filamin-actin asters that can self-organize into a contractile actomyosin network. We suggest that such networks are critical for connecting distant parts of the cell to maintain the mechanical coherence of the cytoplasm.
Materialart:
Online-Ressource
ISSN:
1540-8140
,
0021-9525
DOI:
10.1083/jcb.201210123
DOI:
10.1083/jcb.201210123.dv
Sprache:
Englisch
Verlag:
Rockefeller University Press
Publikationsdatum:
2013
ZDB Id:
1421310-2
SSG:
12
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