In:
Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 78, No. 5 ( 1995-09-01), p. 1173-1177
Abstract:
We have developed an improved method, using 96-well microtiter plates, for the microbiological assay of folic acid. With this method, the tedium of conventional microbiological analysis is substantially decreased. Culture volumes have been reduced 33-fold, and pipetting procedures have been simplified. Assay time has been reduced to 14 h, and sensitivity has increased 10-fold (0.1 ng/mL). Analytical recoveries range from 98 to 104%. Intra-assay and interassay variabilities are less than 11%. The assay does not require extensive manipulation of inoculum. Day-to-day variability has been minimized by using saline aliquots of the bacterial suspension stored at 4°C. The procedure is accurate, selective, and useful for direct measurement of folic acid in multivitamin formulations.
Type of Medium:
Online Resource
ISSN:
1060-3271
,
1944-7922
DOI:
10.1093/jaoac/78.5.1173
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1995
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