In:
Journal of Medical Microbiology, Microbiology Society, Vol. 55, No. 4 ( 2006-04-01), p. 387-392
Abstract:
Between January and April 2002, a total of 271 strains of Staphylococcus aureus were isolated from clinical specimens at Toho University Omori Hospital, Japan, including 201 (74·2 %) which were identified as meticillin-resistant S. aureus (MRSA). However, 34 (12·5 %) were biochemically atypical, because they did not produce acid on mannitol salt agar or did not agglutinate in Staphaurex testing but were categorized as MRSA by PCR analysis and by antibiotic susceptibility. Three automatic identification systems, AutoScan-4 ® (Dade Behring), BD Phoenix ™ (Becton Dickinson) and Vitek ® 2 (bioMérieux), were evaluated by testing these atypical S. aureus isolates. The AutoScan-4 ® and Phoenix ™ systems identified all 34 isolates as S. aureus . Without additional tests such as Staphaurex, observation of colony pigment and haemolysins on sheep blood agar, Vitek ® 2 identified only 16 isolates (47·1 %) as S. aureus with good or better confidence levels and misidentified one of the remaining isolates as Staphylococcus chromogenes . This study shows that it is possible to identify these physiologically atypical S. aureus isolates correctly by using the Phoenix ™ and AutoScan-4 ® fully automatic identification systems.
Type of Medium:
Online Resource
ISSN:
0022-2615
,
1473-5644
DOI:
10.1099/jmm.0.46231-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2006
detail.hit.zdb_id:
2083944-3
SSG:
12
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