In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 10 ( 2022-10-27), p. e0275342-
Abstract:
The entomopathogenic nematode, Heterorhabditis indica , is a popular biocontrol agent of high commercial significance. It possesses tremendous genetic architecture to survive desiccation stress by undergoing anhydrobiosis to increase its lifespan—an attribute exploited in the formulation technology. The comparative transcriptome of unstressed and anhydrobiotic H . indica revealed several previously concealed metabolic events crucial for adapting towards the moisture stress. During the induction of anhydrobiosis in the infective juveniles (IJ), 1584 transcripts were upregulated and 340 downregulated. As a strategy towards anhydrobiotic survival, the IJ showed activation of several genes critical to antioxidant defense, detoxification pathways, signal transduction, unfolded protein response and molecular chaperones and ubiquitin-proteasome system. Differential expression of several genes involved in gluconeogenesis - β -oxidation of fatty acids , glyoxylate pathway ; glyceroneogenesis; fatty acid biosynthesis; amino-acid metabolism - shikimate pathway , sachharopine pathway , kyneurine pathway , lysine biosynthesi s; one-carbon metabolism— polyamine pathway , transsulfuration pathway , folate cycle , methionine cycle , nucleotide biosynthesis ; mevalonate pathway; and glyceraldehyde-3-phosphate dehydrogenase were also observed. We report the role of shikimate pathway, sachharopine pathway and glyceroneogenesis in anhydrobiotes, and seven classes of repeat proteins, specifically in H . indica for the first time. These results provide insights into anhydrobiotic survival strategies which can be utilized to strengthen the development of novel formulations with enhanced and sustained shelf-life.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0275342
DOI:
10.1371/journal.pone.0275342.g001
DOI:
10.1371/journal.pone.0275342.g002
DOI:
10.1371/journal.pone.0275342.g003
DOI:
10.1371/journal.pone.0275342.g004
DOI:
10.1371/journal.pone.0275342.g005
DOI:
10.1371/journal.pone.0275342.g006
DOI:
10.1371/journal.pone.0275342.g007
DOI:
10.1371/journal.pone.0275342.g008
DOI:
10.1371/journal.pone.0275342.g009
DOI:
10.1371/journal.pone.0275342.t001
DOI:
10.1371/journal.pone.0275342.t002
DOI:
10.1371/journal.pone.0275342.t003
DOI:
10.1371/journal.pone.0275342.t004
DOI:
10.1371/journal.pone.0275342.s001
DOI:
10.1371/journal.pone.0275342.s002
DOI:
10.1371/journal.pone.0275342.s003
DOI:
10.1371/journal.pone.0275342.s004
DOI:
10.1371/journal.pone.0275342.s005
DOI:
10.1371/journal.pone.0275342.s006
DOI:
10.1371/journal.pone.0275342.s007
DOI:
10.1371/journal.pone.0275342.s008
DOI:
10.1371/journal.pone.0275342.s009
DOI:
10.1371/journal.pone.0275342.s010
DOI:
10.1371/journal.pone.0275342.s011
DOI:
10.1371/journal.pone.0275342.s012
DOI:
10.1371/journal.pone.0275342.s013
DOI:
10.1371/journal.pone.0275342.s014
DOI:
10.1371/journal.pone.0275342.s015
DOI:
10.1371/journal.pone.0275342.s016
DOI:
10.1371/journal.pone.0275342.s017
DOI:
10.1371/journal.pone.0275342.s018
DOI:
10.1371/journal.pone.0275342.s019
DOI:
10.1371/journal.pone.0275342.s020
DOI:
10.1371/journal.pone.0275342.s021
DOI:
10.1371/journal.pone.0275342.s022
DOI:
10.1371/journal.pone.0275342.s023
DOI:
10.1371/journal.pone.0275342.s024
DOI:
10.1371/journal.pone.0275342.s025
DOI:
10.1371/journal.pone.0275342.s026
DOI:
10.1371/journal.pone.0275342.s027
DOI:
10.1371/journal.pone.0275342.s028
DOI:
10.1371/journal.pone.0275342.s029
DOI:
10.1371/journal.pone.0275342.s030
DOI:
10.1371/journal.pone.0275342.r001
DOI:
10.1371/journal.pone.0275342.r002
DOI:
10.1371/journal.pone.0275342.r003
DOI:
10.1371/journal.pone.0275342.r004
DOI:
10.1371/journal.pone.0275342.r005
DOI:
10.1371/journal.pone.0275342.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
Permalink