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  • 1
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    PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum) TERHADAP DIAMETER DAN TEBAL EPITEL TUBULUS SEMINIFERUS MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
    Universitas Airlangga ; 2020
    In:  Ovozoa : Journal of Animal Reproduction Vol. 8, No. 2 ( 2020-04-07), p. 132-
    Details
    In: Ovozoa : Journal of Animal Reproduction, Universitas Airlangga, Vol. 8, No. 2 ( 2020-04-07), p. 132-
    Abstract: The aim of this research is to analyze the effect of kebar grass on the diameter and epithelium thickness of seminiferous tubules. Twenty male mice of BALB/C strain with an average weight of 25 gram were used in this research. Total five experimental groups (4 mice each group) were treated with combination of TCDD and kebar grass extract designed as follows: (C-) 0mg/g BW/day dan 0µg/kg BW, (C+) 0mg/g BW/day and 7µg/kg BW, (T1) 0,045mg/g BW/day and 7µg/kg BW, (T2) 0,080mg/g BW/day and 7µg/kg BW, (T3) 0,1350mg/g BW/day and 7µg/kg BW. The TCDD was injected peritoneally in single dose while kebar grass extract was given orally everyday for 53 days. The results showed a significant difference (p 〈 0,05) on group which is treated with kebar grass extract 0,135mg/ g BW/ day is proven could maintain the diameter of seminiferous tubules in T3 group (170,5μmb ± 0,65μm) compared with C+ group (129,50μma ± 8,42μm). Significant difference of epithelial thickness were also found between the control and treatment groups (p 〈 0,05). The C+ group (33,57μmb ± 1,61μm) was different from the T1 group (43,80μmc ± 1,46μm), T2 (48,49μmd ± 1,08μm), T3 (52,15μme ± 0,86μm) and C- (56,17μma ± 1,35μm). This findings might suggest TCDD has the potential to decrease the epithelium thickness and the diameter of seminiferous tubules, while kebar grass extract in various dose were proven to be able maintain the epithelium thickness of seminiferous tubules but could not maintain the diameter of seminiferous tubules.
    Type of Medium: Online Resource
    ISSN: 2302-6464
    URL: Article
    DOI: 10.20473/ovz.v8i2.2019.132-138
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2020
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  • 2
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    THE EFFECT OF WATERMELON (Citrullus lanatus) RIND ETHANOLIC EXTRACT ON THE NUMBER OF LEYDIG, SERTOLI, AND SPERMATOGENIC CELLS OF RAT (Rattus novergicus) EXPOSED TO HEAT
    Universitas Airlangga ; 2021
    In:  Ovozoa Journal of Animal Reproduction Vol. 10, No. 1 ( 2021-04-01), p. 7-
    Details
    In: Ovozoa Journal of Animal Reproduction, Universitas Airlangga, Vol. 10, No. 1 ( 2021-04-01), p. 7-
    Abstract: High temperatures lead to oxidative stress, which can disturb spermatogenesis process. Watermelon (Citrullus lanatus) peel contain antioxidant expected to compensate oxidative stress due to heat stress exposure. This study aimed to determine the effect of watermelon rind ethanolic extract on the number of Leydig, Sertoli, and spermatogenic cells of rats exposed to heat (40°C). Twenty rats (Rattus norvegicus) were divided randomly into five groups. In the control group (T0) rats were not exposed to heat nor given the watermelon rind extract. T1, T2, T3, and T4 groups were exposed to heat for an hour daily and orally given placebo (1% Na CMC), 100, 200, and 400 mg/kg BW of watermelon rind extract (in 1% Na CMC). Rats were treated for 52 days, and sacrificed for the testicle collection. Hematoxylin-eosin stained histological slides were prepared for the examination of Leydig, Sertoli and spermatogenic cells. The results showed no significant difference (p 〉 0.05) in the average number of Leydig cells in rats among groups. The number of Sertoli cells and spermatogenic cells of rats exposed to heat (T1) was lower than those of the normal rats (T0 group). The dose of watermelon rind ethanolic extract at 200 mg/kg BW (T3 group) and 400 mg/kg BW (T4) increased (p 〈 0.05) the number of Sertoli and spermatogenic cells. It could be concluded that a dose of 400mg/kg BW of watermelon rind ethanolic extract maintained the number of Leydig cells, Sertoli cells, and spermatogenic cells of rats exposed to heat.
    Type of Medium: Online Resource
    ISSN: 2722-967X , 2302-6464
    URL: Article
    DOI: 10.20473/ovz.v10i1.2021.7-11
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2021
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  • 3
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    PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN
    Universitas Airlangga ; 2020
    In:  Ovozoa : Journal of Animal Reproduction Vol. 8, No. 2 ( 2020-04-07), p. 127-
    Details
    In: Ovozoa : Journal of Animal Reproduction, Universitas Airlangga, Vol. 8, No. 2 ( 2020-04-07), p. 127-
    Abstract: The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.
    Type of Medium: Online Resource
    ISSN: 2302-6464
    URL: Article
    DOI: 10.20473/ovz.v8i2.2019.127-131
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2020
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  • 4
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    KEMAMPUAN MALARISIDAL NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI TERAPI AJUVAN PADA HISTOPATOLOGI HATI MENCIT (Mus musculus) PENDERITA MALARIA
    Universitas Airlangga ; 2020
    In:  Journal of Basic Medical Veterinary Vol. 9, No. 1 ( 2020-08-01), p. 46-
    Details
    In: Journal of Basic Medical Veterinary, Universitas Airlangga, Vol. 9, No. 1 ( 2020-08-01), p. 46-
    Abstract: The aimed of this study is to know malaricidal ability of nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy on liver histopathological of mice (Mus musculus) which infected by Plasmodium berghei. This research uses 30 mice which are divided into six treatment groups namely K0 (not infected without treated), K+ (infected without treated), K1 (infected and treated with chloroquin), K2 (infected and treated with juwet leaf extract), P1 (infected and treated with juwet leaf extract nanoparticle) and P2 (infected and combination treated with chloroquine and juwet leaf extract nanoparticle) which infected by 1x105 in 0.2 ml of Plasmodium berghei. The data were analyzed by Kruskal Wallis using SPSS and followed with Mann-Whitney test. This research result showed there was significant difference (p 〈 0.05) between K1 and P2, but did not showed significant difference (p 〉 0.05) with K+, K2 and P1. Based on this research, it could be concluded that nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy estimated can increase malaricidal ability in degeneration, necrosis, portal inflammation, Kupffer cell hyperplasia, sinusoidal congestion, and hemosiderosis in mice liver which infected by Plasmodium berghei.
    Type of Medium: Online Resource
    ISSN: 2774-9959 , 2302-6820
    URL: Article
    DOI: 10.20473/.v9i1.21094
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2020
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  • 5
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    The Anthelmintic Activity Of Etanol Extract of Porang Tubers Against Fasciola gigantica In Vitro
    Universitas Airlangga ; 2021
    In:  Journal of Basic Medical Veterinary Vol. 10, No. 2 ( 2021-11-30), p. 66-
    Details
    In: Journal of Basic Medical Veterinary, Universitas Airlangga, Vol. 10, No. 2 ( 2021-11-30), p. 66-
    Abstract: This study aims to determine the anthelmintic activity ethanol extract of porang tubers (A. oncophillus) against F. gigantica worms in vitro. There were five treatments and each treatment was done in five replications and used 10 F. gigantica. The treatments of this research were K- with CMC Na 1%, K+ with Albendazole 2.4 mg/ml, P1 with extract concentration 5%, P2 with extract concentration 10%, P3 with extract concentration 20%. The results showed that the extract of Porang tuber (A. oncophillus) had an anthelmintic effect against F. gigantica worms in vitro. In the extract with a concentration of 20%, there were anthelmintic properties that almost the same as Albendazole. The higher the extract concentration, the higher the anthelmintic properties. The longer the immersion time, the higher the number of dead worms. The morphological changes was evaluated by light microscopic examination and the results showed many histopathological changes on the morphology of F. gigantica. The results indicate that the possible use of the tubers as a potential anthelmintic against F. gigantica.
    Type of Medium: Online Resource
    ISSN: 2774-9959 , 2302-6820
    URL: Article
    DOI: 10.20473/jbmv.v10i2.31235
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2021
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  • 6
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    EFEKTIVITAS PEMBERIAN NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI ADJUVANT TERAPI TERHADAP GAMBARANHISTOPATOLOGI PULMO MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium bhergei
    Universitas Airlangga ; 2020
    In:  Journal of Basic Medical Veterinary Vol. 9, No. 1 ( 2020-08-01), p. 22-
    Details
    In: Journal of Basic Medical Veterinary, Universitas Airlangga, Vol. 9, No. 1 ( 2020-08-01), p. 22-
    Abstract: The purpose of this study was to learn how to use juwet nanoparticle leaf extract (Syzygium cumini) as an adjunct therapy in pulmonary histopathological mice (Mus musculus) supported by Plasmodium berghei. This study used 30 rats divided into six groups consisting of K0 (disagree without agreement), P1 (fight without agreement), P2 (fight and dispute with chloroquine), P3 (fight and talk with juwet leaf extract), P4 (useful and useful with juwet leaf extract)) and P5 (extracts and combinations given with chloropine and juwet leaf extract nanoparticles) protected by 1x105 in 0.2 ml of Plasmodium berghei. Data were analyzed by Kruskal Wallis using SPSS and continued with the Mann-Whitney test. The results of this study showed that there were significant differences (p 〈 0.05) between P2 and P5, but did not show a significant difference (p 〉 0.05) with P1, P3 and P4. Based on this study, can be witnessed by juwet nanoparticles (Syzygium Cumini) additional therapeutic leaf extract which is expected to reduce pulmonary damage (hemmoragi, alveolar congestion, edema, hemosiderin) mice (Mus musculus) infected with Plasmodium berghei. 
    Type of Medium: Online Resource
    ISSN: 2774-9959 , 2302-6820
    URL: Article
    DOI: 10.20473/.v9i1.21091
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2020
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  • 7
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    UJI PENETAPAN STABILITAS RETENTION TIME Megestrole acetate DALAM ELUENT MOBILE PHASE MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
    Universitas Airlangga ; 2020
    In:  Journal of Basic Medical Veterinary Vol. 9, No. 1 ( 2020-08-01), p. 37-
    Details
    In: Journal of Basic Medical Veterinary, Universitas Airlangga, Vol. 9, No. 1 ( 2020-08-01), p. 37-
    Abstract: The aim of this research was to determine the level of stability of Megestrole acetate-retention time in storage period for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). The research method used posttest-only control group design by using three treatments and six repetitions. The three repetitions consist into six hours, eight hours and 12 hours. The data were obtained analyzed by Summery Independent T-Test with SPSS 24 for windows. The result showed six hours retention time of Megestrole acetate is stable and eight hours treatment and 12 hours treatment are not stable there is one unstable point of 12 hours treatment that indicates the substance is break down. Based on those result, it could be concluded that the storage time of Megestrole acetate in Eluent Mobile Phase began to show unstable at eight hours of storage.
    Type of Medium: Online Resource
    ISSN: 2774-9959 , 2302-6820
    URL: Article
    DOI: 10.20473/.v9i1.21093
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2020
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  • 8
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    Effect of Combination of Probiotics and Moringa oleifera Leaf Extract on Nutrients Intake in Ducks
    Universitas Airlangga ; 2022
    In:  Jurnal Medik Veteriner Vol. 5, No. 2 ( 2022-10-23), p. 241-246
    Details
    In: Jurnal Medik Veteriner, Universitas Airlangga, Vol. 5, No. 2 ( 2022-10-23), p. 241-246
    Abstract: The purpose of this research was to determine the effect of combination probiotics and Moringa oleifera (M. oleifera) extract in nutrient consumption of ducks. This study used 48 Peking ducks. The concentration of probiotic was 1.2 x 108 CFU/ml. The treatments of this research were P0 (control), P1 (4 ml probiotics), P2 (4 ml M. oleifera extract) and P3 (2 ml probiotics + 2 ml M. oleifera extract). The data were statistically analyzed by Analysis of Variance (ANOVA). The results showed that feed consumption of dry matter, organic matter, crude protein, crude fat, crude fiber and nitrogen free extract were no significant difference (p 〉 0.05) between all treatments. It can be concluded that the addition of combination probiotics and M. oleifera extract can be used to maintain nutrient consumption and safe for health ducks.
    Type of Medium: Online Resource
    ISSN: 2581-012X , 2615-7497
    URL: Article
    DOI: 10.20473/jmv.vol5.iss2.2022.241-246
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2022
    detail.hit.zdb_id: 3142565-3
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  • 9
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    In Vitro antimalarial activity of Syzygium cumini fruit fraction
    ScopeMed ; 2023
    In:  Open Veterinary Journal Vol. 13, No. 9 ( 2023), p. 1116-
    Details
    In: Open Veterinary Journal, ScopeMed, Vol. 13, No. 9 ( 2023), p. 1116-
    Abstract: Background: Malaria is still one of the most severe public health problems worldwide. The development of treatment, prevention, and control of malaria is one of the substantial problems in the world. Aims: To investigate the in vitro antimalarial activity of Syzygium cumin methanol fruit fraction. Methods: Syzygium cumini L fruit powder was macerated with methanol (PA) and the extract obtained was fractionated using the liquid-liquid partition method with n-hexane, ethyl acetate, butanol, chloroform, methanol, and water solvents. In vitro antimalarial assay was conducted using the culture of P. falciparum 3D7 strain culture that had reached 〉 5% growth and was examined for IC 50 values using a 24-well microplate in duplicate. Each treatment and control well contained 1080 µl of complete media. Well number 1 was added with 120 µl fraction, then the solution was diluted until it reached 0.01,0.1, 1,10,100 µg/ml the final concentration in the microtiter well. The control only contained complete media and infected erythrocytes without the addition of anti-malarial drugs. The microplate was incubated for 48 hours. After 48 hours a thin blood smear was made fixed with methanol and stained with 20% Giemsa for 20 minutes to determine the IC 50 value by plotting sample concentrations and percentage of parasitaemia in excel. Results: The IC50 values of ethyl acetate fraction, n.hexane fraction, butanol fraction, and water fraction were 1.189 µg/ml, 76.996 µg/ml, 1,769 µg/ml, and 15.058 µg/ml respectively. Whereases the IC50 values of C1 fraction (mix fraction from chloroform: methanol 100:0 and 90:10) and C4 fraction (mix fraction from chloroform: methanol 20:80, 10:90 and 0:100) were 100.126 µg/ml and 1.015 µg/ml respectively. The results showed that the IC50 value of ethyl acetate, butanol, and C4 fraction were lower than 10µg/ml and were considered as good activity (strong antimalarial activity). Conclusion: The ethyl acetate, butanol and C4 subfraction from Syzygium cumini fruit have the potential to be developed as an antimalarial agent.
    Type of Medium: Online Resource
    ISSN: 2226-4485
    URL: Journal
    URL: Article
    DOI: 10.5455/OVJ.
    DOI: 10.5455/OVJ.2023.v13.i9.7
    Language: Unknown
    Publisher: ScopeMed
    Publication Date: 2023
    detail.hit.zdb_id: 2651664-0
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  • 10
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    Hematologic changes and splenic index on malaria mice models given Syzygium cumini extract as an adjuvant therapy
    Veterinary World ; 2019
    In:  Veterinary World Vol. 12, No. 1 ( 2019-1), p. 106-111
    Details
    In: Veterinary World, Veterinary World, Vol. 12, No. 1 ( 2019-1), p. 106-111
    Abstract: Aim: This research aimed to determine the efficacy of Syzygium cumini L. as an adjuvant therapy on blood changes and splenic index of mice model malaria. Materials and Methods: Mice were infected intraperitoneally with 0.2 ml red blood cell (RBC) that contains 1×106 Plasmodium berghei. 35 mice were divided into seven treatment groups: Group K0: Mice were not infected; K1: Mice were infected; K2: Mice were infected and given chloroquine; P1: Mice were infected and given S. cumini leaf extract; P2: Mice were infected and given chloroquine and also S. cumini leaf extract; P3: Mice was infected and given S. cumini stem bark extract; and P4: Mice were infected and given chloroquine and S. cumini stem bark extract. Treatment was given for 4 days 24 h post-P. berghei infection. 21st day post-P. berghei infection, blood was taken from the heart for hematological examination, and the spleen was taken to examine the splenic index and also to measure the weight and length of the spleen. Hematological data and splenic index were analyzed by analysis of variance test, and if there is a difference, the test is continued by Duncan's multiple range test with 5% level. Results: The K0 group has normal hemoglobin (HGB), RBC, and hematocrit (HCT) and significantly different (p 〈 0.05) than other groups. HGB, RBC, and HCT of K1 group were under normal range, lowest, and significantly different (p 〈 0.05) than other groups. Mean corpuscular volume and mean corpuscular HGB values of K2 groups showed a decrease. The number of leukocytes, lymphocytes, and monocytes of K1 groups was increasing and significantly different (p 〈 0.05) with K2 and treatment group. The length, width, weight, and splenic index of K1 group were significantly different (p 〈 0.05) with K0 group. K2 and treatment groups showed that the length and width of spleens were significantly different (p 〈 0.05) with K1. Conclusion: The combination of chloroquine with leaf and chloroquine with stem bark extract of S. cumini as adjuvant therapy may increase the amount of erythrocyte; decrease the number of leukocytes, lymphocytes, and monocytes; and decrease the length, width, and splenic index on malaria mice models.
    Type of Medium: Online Resource
    ISSN: 2231-0916 , 0972-8988
    URL: Journal
    URL: Article
    DOI: 10.14202/vetworld.
    DOI: 10.14202/vetworld.2019.106-111
    Language: English
    Publisher: Veterinary World
    Publication Date: 2019
    detail.hit.zdb_id: 2456277-4
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