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  • 1
    In: Journal of Mass Spectrometry, Wiley, Vol. 40, No. 2 ( 2005-02), p. 214-226
    Abstract: Recent work on protein and peptide biomarker patterns revealed the difficulties in identifying their molecular components, which is indispensable for validation of the biological context. Cerebrospinal fluid and brain tissue are used as sources to discover new biomarkers, e.g. for neurodegenerative diseases. Many of these biomarker candidates are peptides with a molecular mass of 〈 10 kDa. Their identification is favourably achieved with a ‘top‐down’ approach, because this requires less purification and an enzymatic cleavage will often not yield enough specific fragments for successful database searches. Here, we describe an approach using quadrupole time‐of‐flight mass spectrometry (TOFMS) as a highly efficient mass spectrometric purification and identification tool after off‐line decomplexation of biological samples by liquid chromatography. After initial peptidomic screening with matrix‐assisted laser desorption/ionization (MALDI) TOFMS, the elution behaviour in chromatography and the exact molecular mass were used to locate the same signals in nanoelectrospray measurements. Most of the peaks detected in MALDI‐TOFMS could be retrieved in nanoelectrospray quadrupole TOFMS. Suitable collision energies for informative fragment spectra were investigated for different parent ions, charge states and molecular masses. After collision‐induced dissociation, the resulting fragmentation data of multiply charged ions can become much more complicated than those derived from tryptic peptide digests. However, the mass accuracy and resolution of quadrupole TOF instruments results in high‐quality data suitable for determining peptide sequences. The protein precursor, proteolytic processing and post‐translational modifications were identified by automated database searches. This is demonstrated by the exemplary identifications of thymosin beta‐4 (5.0 kDa) and NPY (4.3 kDa) from rat hypothalamic tissue and ubiquitin (8.6 kDa) from human cerebrospinal fluid. The high data quality should also allow for de novo identification. This methodology is generally applicable for peptides up to a molecular mass of about 10 kDa from body fluids, tissues or other biological sources. Copyright © 2005 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 1076-5174 , 1096-9888
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2005
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  • 2
    In: Journal of Sports Sciences, Informa UK Limited, Vol. 38, No. 2 ( 2020-01-17), p. 130-139
    Type of Medium: Online Resource
    ISSN: 0264-0414 , 1466-447X
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2020
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  • 3
    In: Rapid Communications in Mass Spectrometry, Wiley, Vol. 15, No. 17 ( 2001-09-15), p. 1586-1592
    Abstract: Peptides with biological functions often contain disulfide bridges connecting two cysteine residues. In an attempt to screen biological fluids for peptides containing cysteine residues, we have developed a sensitive and specific method to label cysteines selectively and detect the resulting molecular mass shift by differential mass spectrometry. First, reduction of disulfide bridges and carboxyamidomethylation of free thiols is adjusted to quantitatively achieve cysteine alkylation for complex peptide extracts. In a second step, matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) before and after chemical derivatization is performed, followed by differential analysis to determine shifted peaks; shifted peaks belong to cysteine‐containing peptides, other peaks remain unchanged. The number of cysteines can then be determined by the resulting molecular mass shift. Free, reduced cysteines are shifted by 57 u, two oxidized cysteines involved in disulfide bridges (cystine) result in a shift to higher mass per disulfide bridge of 116 u. Disulfide bridges connecting different amino acid chains like insulin break up during reduction. In this case, two peaks with lower molecular masses result from a single one in the unmodified sample. With this technique, we were able to identify cysteine‐containing peptides and short fragments of proteins present in human blood filtrate. Copyright © 2001 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0951-4198 , 1097-0231
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2001
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  • 4
    In: Atmospheric Measurement Techniques, Copernicus GmbH, Vol. 16, No. 5 ( 2023-03-17), p. 1443-1460
    Abstract: Abstract. A new global O3 data product retrieved from the Michelson Interferometer for Passive Atmospheric Sounding (MIPAS) spectra with the IMK–IAA MIPAS data processor has been released. These data are based on ESA version 8 recalibrated radiance spectra with improved temporal stability. Changes in the level-2 processing with respect to previous data versions include the following: (1) the retrievals use improved temperature data and thus suffer less from the propagation of related errors. (2) The background continuum is now considered up to 58 km. (3) A priori information is now used to constrain the retrieval of the radiance offset. (4) Water vapour is fitted jointly with ozone to minimize the impact of interfering water lines. (5) A more adequate regularization has been chosen. (6) Ozone absorption lines in the MIPAS A band (685–980 cm−1) are used almost exclusively because of inconsistencies in spectroscopic data in the MIPAS AB band (1010–1180 cm−1). Only at altitudes above 50 km, where A-band ozone lines do not provide sufficient information, are ozone lines in the MIPAS AB band used. (7) Temperature-adjusted climatologies of vibrational temperatures of O3 and CO2 are considered to account for non-local thermodynamic equilibrium radiation. Ozone errors are estimated to be less than 10 % in the altitude range 20–50 km. The error budget is dominated by the spectroscopic errors, followed by the uncertainty of the instrumental line shape function, the gain calibration error, and the spectral noise. The error contribution of interfering gases is almost negligible. The vertical resolution depends on altitude and atmospheric conditions. In 2002–2004 it varies between 2.5 km at the lowest altitudes and 6 km at 70 km, while in 2005–2012 it covers 2 to 5.5 km in the same altitude range. The horizontal smearing in terms of the full width at half maximum of the horizontal component of the two-dimensional averaging kernel matrix is smaller than, or approximately equal to, the distance between two subsequent limb scans at all altitudes. This implies that the horizontal resolution is sampling-limited or optimal, respectively. An additional data version is made available that is free of the formal a priori information and thus more user-friendly for certain applications. Version 8 ozone results show a better consistency between the two MIPAS measurement periods. They seem to be more realistic than preceding data versions in terms of long-term stability, as at least a part of the drift is corrected. Further, the representation of elevated stratopause situations is improved.
    Type of Medium: Online Resource
    ISSN: 1867-8548
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2023
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  • 5
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2017
    In:  Journal of Strength and Conditioning Research Vol. 31, No. 3 ( 2017-03), p. 608-619
    In: Journal of Strength and Conditioning Research, Ovid Technologies (Wolters Kluwer Health), Vol. 31, No. 3 ( 2017-03), p. 608-619
    Abstract: Julian, R, Meyer, T, Fullagar, HHK, Skorski, S, Pfeiffer, M, Kellmann, M, Ferrauti, A, and Hecksteden, A. Individual patterns in blood-borne indicators of fatigue—trait or chance. J Strength Cond Res 31(3): 608–619, 2017—Blood-borne markers of fatigue such as creatine kinase (CK) and urea (U) are widely used to fine-tune training recommendations. However, predictive accuracy is low. A possible explanation for this dissatisfactory characteristic is the propensity of athletes to react to different patterns of fatigue indicators (e.g., predominantly muscular [CK] or metabolic [U] ). The aim of the present trial was to explore this hypothesis by using repetitive fatigue-recovery cycles. A total of 22 elite junior swimmers and triathletes (18 ± 3 years) were monitored for 9 weeks throughout 2 training phases (low-intensity, high-volume [LIHV] and high-intensity, low-volume [HILV] phases). Blood samples were collected each Monday (recovered) and Friday (fatigued) morning. From measured values of CK, U, free-testosterone (FT), and cortisol (C) as determined in the rested and fatigued state, respectively, Monday–Friday differences (Δ) were calculated and classified by magnitude before calculation of ratios (ΔCK/ΔU and ΔFT/ΔC). Coefficient of variation (CV) was calculated as group-based estimate s of reproducibility. Linear mixed modeling was used to differentiate inter- and intraindividual variability. Consistency of patterns was analyzed by comparing with threshold values ( 〈 0.9 or 〉 1.1 for all weeks). Reproducibility was very low for fatigue-induced changes (CV ≥ 100%) with interindividual variation accounting for 45–60% of overall variability. Case-wise analysis indicated consistent ΔCK/ΔU patterns for 7 individuals in LIHV and 7 in HILV; 5 responded consistently throughout. For ΔFT/ΔC the number of consistent patterns was 2 in LIHV and 3 in HILV. These findings highlight the potential value of an individualized and multivariate approach in the assessment of fatigue.
    Type of Medium: Online Resource
    ISSN: 1064-8011
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2017
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  • 6
    In: Nature Precedings, Springer Science and Business Media LLC
    Abstract: Owing to its availability, ease of collection and correlation with (patho-) physiology, urine is an attractive source for clinical proteomics. However, the lack of comparable datasets from large cohorts has greatly hindered development in this field. Here we report the establishment of a high resolution proteome database of naturally occurring human urinary peptides and proteins - ranging from 800-17,000 Da - from over 3,600 individual samples using capillary electrophoresis coupled to mass spectrometry, yielding an average of 1,500 peptides per sample. All processed data were deposited in an SQL database, currently containing 5,010 relevant unique urinary peptides that serve as classifiers for diagnosis and monitoring of diseases, including kidney and vascular diseases. Of these, 352 have been sequenced to date. To demonstrate the applicability of this database, two examples of disease diagnosis were provided: For renal damage diagnosis, patients with a specific renal disease were identified with high specificity and sensitivity in a blinded cohort of 131 individuals. We further show definition of biomarkers specific for immunosuppression and complications after transplantation (Kaposi’s sarcoma). Due to its high information content, this database will be a powerful tool for the validation of biomarkers for both renal and non-renal diseases.
    Type of Medium: Online Resource
    ISSN: 1756-0357
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2007
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  • 7
    Online Resource
    Online Resource
    Hogrefe Publishing Group ; 2015
    In:  Zeitschrift für Sportpsychologie Vol. 22, No. 4 ( 2015-10), p. 146-162
    In: Zeitschrift für Sportpsychologie, Hogrefe Publishing Group, Vol. 22, No. 4 ( 2015-10), p. 146-162
    Abstract: Zusammenfassung. Mit der Entwicklung einer Kurzform (Kurzskala zur Erfassung von Erholung und Beanspruchung im Sport; KEB) des Akutmaßes zur Erfassung von Erholung und Beanspruchung im Sport (AEB) wird dem Bedarf der Sportpraxis nach einem ökonomischen, validen und veränderungssensitiven Messinstrument zur Erfassung von Erholung und Beanspruchung nachgegangen. Nach einem vierstufigen Entwicklungs- und Validierungsprozess des AEB wurde aus den exploratorisch errechneten und konfirmatorisch geprüften Faktoren des AEB die KEB mit acht Items abgeleitet. Es konnten sehr gute Bereichshomogenitäten, moderate Interkorrelationen und hypothesenkonforme Korrelationen mit dem AEB berechnet werden. Der KEB zeigte ein dem AEB inhaltlich übereinstimmendes Korrelationsmuster mit den konvergenten Verfahren Erholungs-Belastungs-Fragebogen für Sportler (EBF-Sport) und visuelle Analogskala zum Muskelschmerzempfinden (Delayed-onset muscle soreness; DOMS). In einem Trainingsmonitoring mit der deutschen Hockey-Juniorinnen-Nationalmannschaft wurde die Veränderungssensitivität der KEB belegt und ein übereinstimmendes Ergebnismuster mit dem AEB, dem EBF-Sport und der DOMS gefunden.
    Type of Medium: Online Resource
    ISSN: 1612-5010 , 2190-6300
    Language: German
    Publisher: Hogrefe Publishing Group
    Publication Date: 2015
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  • 8
    Online Resource
    Online Resource
    Frontiers Media SA ; 2021
    In:  Frontiers in Psychology Vol. 12 ( 2021-3-25)
    In: Frontiers in Psychology, Frontiers Media SA, Vol. 12 ( 2021-3-25)
    Abstract: Self-applied portable polysomnography is considered a promising tool to assess sleep architecture in field studies. However, no findings have been published regarding the appearance of a first-night effect within a sport-specific setting. Its absence, however, would allow for a single night sleep monitoring and hence minimize the burden on athletes while still obtaining the most important variables. For this reason, the aim of the study was to assess whether the effect appears in home-based sleep monitoring of elite athletes. The study sample included eight male and 12 female German elite athletes from five different sports. Participants slept with a portable polysomnography for two nights, which they self-applied at night before going to bed. Time in bed and wake-up time in the morning were freely chosen by each individual athlete without any restrictions regarding time or sleeping environment. Participants were asked to keep the same location and time frame during the two days of monitoring and stick to their usual sleeping schedules. Sleep stages were manually scored using 30-s epochs. Sleep parameters and stages were later compared with the help of linear mixed models to investigate the factor time. Significant differences between the two nights were found for percentage of Non-REM sleep [T(19) = −2,10, p & lt; 0.05, d = −0.47, 95%-CI (−7.23, −0.01)] with small effect size, Total Wake Time [T(19) = 2.30, p = 0.03, d = 0.51, 95%-CI (1.66, 35.17)], Sleep Efficiency [T(19) = −2.48, p = 0.02, d = −0.55, 95%-CI (−7.43, −0.63)], and Wake percentage [T(19) = 2.47, p = 0.02, d = 0.55, 95%-CI (0.61, 7.43)] with moderate effect sizes, and N3 Sleep Onset Latency [T(19) = 3.37, p & lt; 0.01, d = 0.75, 95%-CI (7.15, 30.54)] with large effect size. Confidence Intervals for all other indices range from negative to positive values and hence specify, that parameters were not systematically negatively affected in the first night. Findings suggest that some individuals are more affected by the first-night effect than others. Yet, in order to keep the measurement uncertainties to a minimum, a more conservative approach with at least two monitoring nights should be used whenever possible, if no other supporting information on the athletes says otherwise.
    Type of Medium: Online Resource
    ISSN: 1664-1078
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
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  • 9
    In: Frontiers in Physiology, Frontiers Media SA, Vol. 9 ( 2018-4-18)
    Type of Medium: Online Resource
    ISSN: 1664-042X
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2018
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  • 10
    In: Atmospheric Chemistry and Physics, Copernicus GmbH, Vol. 18, No. 12 ( 2018-06-25), p. 8873-8892
    Abstract: Abstract. We present model simulations with the atmospheric chemistry–climate model ECHAM5/MESSy Atmospheric Chemistry (EMAC) nudged toward European Centre for Medium-Range Weather Forecasts (ECMWF) ERA-Interim reanalyses for the Arctic winters 2009/2010 and 2010/2011. This study is the first to perform an extensive assessment of the performance of the EMAC model for Arctic winters as previous studies have only made limited evaluations of EMAC simulations which also were mainly focused on the Antarctic winter stratosphere. We have chosen the two extreme Arctic winters 2009/2010 and 2010/2011 to evaluate the formation of polar stratospheric clouds (PSCs) and the representation of the chemistry and dynamics of the polar winter stratosphere in EMAC. The EMAC simulations are compared to observations by the Michelson Interferometer for Passive Atmospheric Soundings (Envisat/MIPAS) and the observations from the Aura Microwave Limb Sounder (Aura/MLS). The Arctic winter 2010/2011 was one of the coldest stratospheric winters on record, leading to the strongest depletion of ozone measured in the Arctic. The Arctic winter 2009/2010 was, from the climatological perspective, one of the warmest stratospheric winters on record. However, it was distinguished by an exceptionally cold stratosphere (colder than the climatological mean) from mid-December 2009 to mid-January 2010, leading to prolonged PSC formation and existence. Significant denitrification, the removal of HNO3 from the stratosphere by sedimentation of HNO3-containing polar stratospheric cloud particles, occurred in that winter. In our comparison, we focus on PSC formation and denitrification. The comparisons between EMAC simulations and satellite observations show that model and measurements compare well for these two Arctic winters (differences for HNO3 generally within ±20 %) and thus that EMAC nudged toward ECMWF ERA-Interim reanalyses is capable of giving a realistic representation of the evolution of PSCs and associated sequestration of gas-phase HNO3 in the polar winter stratosphere. However, simulated PSC volume densities are smaller than the ones derived from Envisat/MIPAS observations by a factor of 3–7. Further, PSCs in EMAC are not simulated as high up (in altitude) as they are observed. This underestimation of PSC volume density and vertical extension of the PSCs results in an underestimation of the vertical redistribution of HNO3 due to denitrification/re-nitrification. The differences found here between model simulations and observations stipulate further improvements in the EMAC set-up for simulating PSCs.
    Type of Medium: Online Resource
    ISSN: 1680-7324
    Language: English
    Publisher: Copernicus GmbH
    Publication Date: 2018
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