In:
Microbial Physiology, S. Karger AG, Vol. 9, No. 2 ( 2005), p. 110-120
Abstract:
In the present study, the metabolic pathways involved in the degradation of benzyl alcohol and 1-butanol, the hydrolyzed products of butyl benzyl phthalate, were investigated by the 〈 i 〉 Gordonia 〈 /i 〉 sp. strain MTCC 4818. The strain can utilize both benzyl alcohol and 1-butanol individually as sole carbon sources, where benzyl alcohol was found to be metabolized via benzaldehyde, benzoic acid and catechol, which was further degraded by 〈 i 〉 ortho 〈 /i 〉 -cleavage dioxygenase to 〈 i 〉 cis,cis 〈 /i 〉 -muconic acid and subsequently to muconolactone leading to tricarboxylic acid cycle. On the other hand, 1-butanol was metabolized via butyraldehyde and butyric acid, which was channeled into the tricarboxylic acid cycle via the β-oxidation pathway. Numbers of dehydrogenases, both NAD 〈 sup 〉 + 〈 /sup 〉 -dependent and NAD 〈 sup 〉 + 〈 /sup 〉 -independent, were found to be involved in the degradation of benzyl alcohol and 1-butanol, where several dehydrogenases exhibited relaxed substrate specificity. Both 2,3- and 3,4-dihydroxybenzoic acids were utilized by the test organism for growth and metabolized by the 〈 i 〉 ortho 〈 /i 〉 -cleavage pathway by the cell-free extract of benzoate-grown cells, similar to catechol, suggesting possible broad substrate specificity of the ring cleavage dioxygenase. Moreover, the test organism can utilize various primary and secondary alcohols, aliphatic aldehydes and acids in the C 〈 sub 〉 2 〈 /sub 〉 –C 〈 sub 〉 5 〈 /sub 〉 range besides 〈 i 〉 n 〈 /i 〉 -hexadecane, 1,4-butanediol and cyclohexanol individually as the sole carbon sources indicating metabolic diversity in the 〈 i 〉 Gordonia 〈 /i 〉 sp. strain MTCC 4818.
Type of Medium:
Online Resource
ISSN:
2673-1665
,
2673-1673
Language:
English
Publisher:
S. Karger AG
Publication Date:
2005
detail.hit.zdb_id:
3042601-7
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