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  • 11
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    Online Resource
    Search for hepatopancreatic ecdysteroid-responsive genes during the crayfish molt cycle: from a single gene to multigenicity
    The Company of Biologists ; 2007
    In:  Journal of Experimental Biology Vol. 210, No. 20 ( 2007-10-15), p. 3525-3537
    Details
    In: Journal of Experimental Biology, The Company of Biologists, Vol. 210, No. 20 ( 2007-10-15), p. 3525-3537
    Abstract: The expression of the vitellogenin gene of the red-claw crayfish Cherax quadricarinatus (CqVg) was previously demonstrated in male crayfish during an endocrinologically induced molt cycle. The hypothesis that this expression is under the direct control of ecdysteroids was tested in this study both in vivo and in vitro. Unlike vitellogenin of insects, CqVg was not found to be ecdysteroid-responsive. Thus, a multigenic approach was employed for the identification of other hepatopancreatic ecdysteroid-responsive genes by a cDNA microarray. For the purposes of this study, a multi-parametric molt-staging technique, based on X-ray detection of gastrolith growth, was developed. To identify ecdysteroid-responsive genes during premolt, the molt cycle was induced by two manipulations, 20-hydroxyecdysone administration and X-organ–sinus gland complex removal; both resulted in significant elevation of ecdysteroids. Two clusters of affected genes (129 and 122 genes, respectively) were revealed by the microarray. It is suggested that only genes belonging to similarly responsive (up- or downregulated) gene clusters in both manipulations (102 genes) could be considered putative ecdysteroid-responsive genes. Some of these ecdysteroid-responsive genes showed homology to genes controlling chitin metabolism, proteases and other cellular activities, while 56.8% were unknown. The majority of the genes were downregulated, presumably by an energetic shift of the hepatopancreas prior to ecdysis. The effect of 20-hydroxyecdysone on representative genes from this group was confirmed in vitro using a hepatopancreas tissue culture. This approach for ecdysteroid-responsive gene identification could also be implemented in other tissues for the elucidation of ecdysteroid-specific signaling pathways during the crustacean molt cycle.
    Type of Medium: Online Resource
    ISSN: 1477-9145 , 0022-0949
    URL: Article
    DOI: 10.1242/jeb.006791
    Language: English
    Publisher: The Company of Biologists
    Publication Date: 2007
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    detail.hit.zdb_id: 1482461-9
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  • 12
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    Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis
    The Endocrine Society ; 2009
    In:  Endocrinology Vol. 150, No. 3 ( 2009-03-01), p. 1278-1286
    Details
    In: Endocrinology, The Endocrine Society, Vol. 150, No. 3 ( 2009-03-01), p. 1278-1286
    Abstract: Androgenic glands (AGs) of the freshwater prawn Macrobrachium rosenbergii were subjected to endocrine manipulation, causing them to hypertrophy. Transcripts from these glands were used in the construction of an AG cDNA subtractive library. Screening of the library revealed an AG-specific gene, termed the M. rosenbergii insulin-like AG (Mr-IAG) gene. The cDNA of this gene was then cloned and fully sequenced. The cysteine backbone of the predicted mature Mr-IAG peptide (B and A chains) showed high similarity to that of other crustacean AG-specific insulin-like peptides. In vivo silencing of the gene, by injecting the prawns with Mr-IAG double-stranded RNA, temporarily prevented the regeneration of male secondary sexual characteristics, accompanied by a lag in molt and a reduction in growth parameters, which are typically higher in males of the species. In terms of reproductive parameters, silencing of Mr-IAG led to the arrest of testicular spermatogenesis and of spermatophore development in the terminal ampullae of the sperm duct, accompanied by hypertrophy and hyperplasia of the AGs. This study constitutes the first report of the silencing of a gene expressed specifically in the AG, which caused a transient adverse effect on male phenotypical gender differences and spermatogenesis. Temporal silencing of a newly identified insulin-like gene from prawn androgenic gland inhibits primary spermatogenesis, male secondary sex characteristics, and growth.
    Type of Medium: Online Resource
    ISSN: 0013-7227 , 1945-7170
    URL: Article
    DOI: 10.1210/en.2008-0906
    Language: English
    Publisher: The Endocrine Society
    Publication Date: 2009
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    detail.hit.zdb_id: 2011695-0
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  • 13
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    Intratesticular spermatozoa retention in low fertility ageing roosters is related to malformations of Sertoli cell ectoplasmic specializations
    Wiley ; 1996
    In:  The Journal of Experimental Zoology Vol. 275, No. 4 ( 1996-07-01), p. 317-325
    Details
    In: The Journal of Experimental Zoology, Wiley, Vol. 275, No. 4 ( 1996-07-01), p. 317-325
    Type of Medium: Online Resource
    ISSN: 0022-104X , 1097-010X
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.1002/(SICI)1097-010X(19960701)275:4〈〉1.0.CO;2-T
    DOI: 10.1002/(ISSN)1097-010X
    DOI: 10.1002/(SICI)1097-010X(19960701)275:4〈317::AID-JEZ10〉3.0.CO;2-7
    Language: English
    Publisher: Wiley
    Publication Date: 1996
    detail.hit.zdb_id: 2205981-7
    detail.hit.zdb_id: 3181-1
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  • 14
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    A crayfish molar tooth protein with putative mineralized exoskeletal chitinous matrix c properties
    The Company of Biologists ; 2015
    In:  Journal of Experimental Biology ( 2015-01-01)
    Details
    In: Journal of Experimental Biology, The Company of Biologists, ( 2015-01-01)
    Abstract: Some crustaceans possess exoskeletons that are reinforced with calcium carbonate. In the crayfish Cherax quadricarinatus, the molar tooth, which is part of the mandibular exoskeleton, contains an unusual crystalline enamel-like apatite layer. As this layer resembles vertebrate enamel in composition and function, it offers an interesting example of convergent evolution. Unlike other parts of the crayfish exoskeleton, which is periodically shed and regenerated during the molt cycle, molar mineral deposition takes place during the pre-molt stage. The molar mineral composition transforms continuously from fluorapatite through amorphous calcium phosphate to amorphous calcium carbonate and is mounted on chitin. The process of crayfish molar formation is entirely extracellularand presumably controlled by proteins, lipids, polysaccharides, low-molecular weight molecules and calcium salts. We have identified a novel molar protein termed Cq-M15 from C. quadricarinatus and cloned its transcript from the molar-forming epithelium. Its transcript and differential expression were confirmed by a next generation sequencing library. The predicted acidic pI of Cq-M15 suggests its possible involvement in mineral arrangement. Cq-M15 is expressed in several exoskeletal tissues at pre-molt and its silencing is lethal. Like other arthropod cuticular proteins, Cq-M15 possesses a chitin-binding Rebers-Riddiford domain, with a recombinant version of the protein found to bind chitin. Cq-M15 was also found to interact with calcium ions in a concentration dependent manner. This latter property might make Cq-M15 useful for bone and dental regenerative efforts. We suggest that, in molar, this protein might be involved in calcium phosphate and/or carbonate precipitation.
    Type of Medium: Online Resource
    ISSN: 1477-9145 , 0022-0949
    URL: Article
    DOI: 10.1242/jeb.123539
    Language: English
    Publisher: The Company of Biologists
    Publication Date: 2015
    detail.hit.zdb_id: 1413561-9
    detail.hit.zdb_id: 1482461-9
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  • 15
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    An androgenic gland membrane-anchored gene associated with the crustacean insulin-like androgenic gland hormone
    The Company of Biologists ; 2013
    In:  Journal of Experimental Biology
    Details
    In: Journal of Experimental Biology, The Company of Biologists
    Abstract: Crustacean male sexual differentiation is governed by the androgenic gland (AG) and specifically by the secreted insulin-like AG hormone (IAG), thus far identified in several decapod species including the Australian red claw crayfish Cherax quadricarinatus (termed Cq-IAG). While few insulin-like AG genes have been identified in crustaceans, other AG-specific genes have not been documented until now. In the present study we describe the recent identification of a non-IAG AG-specific transcript obtained from the C. quadricarinatus AG cDNA library. This transcript, termed C. quadricarinatus membrane-anchored AG-specific factor (Cq-MAG), was fully sequenced and found to encode a putative product of 189 amino acids including a signal anchoring peptide. Expression of a recombinant GFP fusion protein lacking the signal anchor encoding sequence dramatically affected recombinant protein localization pattern. While the expression of the deleterious fusion protein was observed throughout most of the cell, the native GFP::Cq-MAG fusion protein was observed mainly surrounding the periphery of the nucleus, demonstrating an ER-like localization pattern. Moreover, co-expressing the wild-type Cq-MAG (fused to GFP) and the Cq-IAG hormone revealed that these peptides indeed co-localize. This study is the first to report a protein specifically associated with the insulin-like androgenic gland hormone in addition to the finding of another AG-specific transcript in crustaceans. Previous knowledge suggests that insulin/insulin-like factor secretion involves tissue-specific transcripts and membrane anchored proteins. On this note, Cq-MAG's tissue specificity, anchoring properties, and intracellular co-localization with Cq-IAG suggest that it may play a role in the processing and secretion of this insulin-like androgenic gland hormone.
    Type of Medium: Online Resource
    ISSN: 1477-9145 , 0022-0949
    URL: Article
    DOI: 10.1242/jeb.080523
    Language: English
    Publisher: The Company of Biologists
    Publication Date: 2013
    detail.hit.zdb_id: 1413561-9
    detail.hit.zdb_id: 1482461-9
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  • 16
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    A Novel Chitin Binding Crayfish Molar Tooth Protein with Elasticity Properties
    Public Library of Science (PLoS) ; 2015
    In:  PLOS ONE Vol. 10, No. 5 ( 2015-5-26), p. e0127871-
    Details
    In: PLOS ONE, Public Library of Science (PLoS), Vol. 10, No. 5 ( 2015-5-26), p. e0127871-
    Type of Medium: Online Resource
    ISSN: 1932-6203
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.1371/journal.pone.0127871
    DOI: 10.1371/journal.pone.0127871.g001
    DOI: 10.1371/journal.pone.0127871.g002
    DOI: 10.1371/journal.pone.0127871.g003
    DOI: 10.1371/journal.pone.0127871.g004
    DOI: 10.1371/journal.pone.0127871.s001
    Language: English
    Publisher: Public Library of Science (PLoS)
    Publication Date: 2015
    detail.hit.zdb_id: 2267670-3
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  • 17
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    Intratesticular retention of sperm and premature decline in fertility in the domestic rooster, Gallus domesticus
    Wiley ; 1995
    In:  Journal of Experimental Zoology Vol. 273, No. 1 ( 1995-09), p. 76-81
    Details
    In: Journal of Experimental Zoology, Wiley, Vol. 273, No. 1 ( 1995-09), p. 76-81
    Abstract: In domestic roosters, which were originally seasonal breeders and that are now kept under unnatural and unchanging conditions throughout the year, fertility peaks at 32 weeks of age (96%) but it subsequently declines rapidly to only 5% at 110 weeks despite the fact that roosters can live for about 10 years. Roosters exhibiting this low‐fertility syndrome have reduced levels of spermatozoa in the ejaculate. Concomitantly, however, superabundant but apparently normal spermatozoa are found attached to Sertoli cells and, in addition, the seminiferous epithelium fails to show evidence of the regression or atrophy that characterizes both aging non‐seasonal breeders and true seasonal breeders during non‐reproductive periods. This syndrome of premature low fertility appears to stem from impaired spermiation with resultant retention of spermatozoa by Sertoli cells. To examine this problem, we compared intratesticular incorporation of 3 H‐thymidine between high‐fertility (32‐week‐old) and low‐fertility (82‐week‐old) roosters. Radioactivity associated with spermatozoa, 33 days post‐injection, was almost 50% higher in the low‐fertility roosters than in the high‐fertility ones. By contrast, both groups showed similar characteristics with respect to a) intratesticular incorporation of 3 H‐thymidine, b) dynamics of spermatogenesis, c) intratesticular level of radioactivity just before the initiation of spermiation, and d) the duration of both spermatogenesis and the time required for sperm to pass through the genital tract. Our results confirm that intratesticular retention of sperm occurs in roosters with premature low‐fertility syndrome and suggest new possibilities for the study of the complex relationship between Sertoli cells and spermatozoa and the effects of this relationship on fertility. © 1995 Wiley‐Liss, Inc.
    Type of Medium: Online Resource
    ISSN: 0022-104X , 1097-010X
    URL: Issue
    URL: Article
    DOI: 10.1002/jez.v273:1
    DOI: 10.1002/jez.1402730110
    Language: English
    Publisher: Wiley
    Publication Date: 1995
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    detail.hit.zdb_id: 3181-1
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  • 18
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    DataSheet_1.docx
    Frontiers Media SA ; 2023
    In:  Frontiers in Marine Science Vol. 10 ( 2023-10-17)
    Details
    In: Frontiers in Marine Science, Frontiers Media SA, Vol. 10 ( 2023-10-17)
    Abstract: Despite extensive research spanning several decades, a commercial cell line for prawns remains elusive, and we have yet to determine the ideal conditions required for successful in vitro cell cultures of crustaceans. This study marks the first application of mass spectrometry to analyze lipid content changes in prawn culture media, allowing us to investigate how specific metabolites in media correlate with cell cycle and proliferation status. To investigate these relationships, short-term cell culture experiments were conducted using three commercial media: Dulbecco's modified eagle medium (DMEM) high glucose, Opti-Minimal essential media (Opti-MEM), and DMEM:F12. We monitored metabolic activity, performed cell-cycle analysis, and used mass spectrometry to analyze lipid metabolic profiles of culture media. This comprehensive approach enables us to examine the interplay between media lipidomic content and cellular behavior. Our results revealed that all three media sustained cellular metabolic activity. DMEM high glucose and Opti-MEM showed peaks in metabolic activity at day 4, while DMEM:F12 exhibited a peak at day 7. Opti-MEM supported the shortest doubling time, making it ideal for short-term culture. Interestingly, DMEM high glucose retained more attached cells on day 1 and had the highest count on day 4, with a noticeable trend towards the G2/M cell-cycle phase. Moreover, our lipidomic analysis of Opti-MEM revealed significantly higher concentrations of 26 annotated lipids compared to the other media. Notably, lipids like phosphatidylcholine and phosphatidylserine were consistently abundant in all three media, and palmitate levels correlated with proliferation phase. These findings have significant implications for establishing sustainable crustacean cell culture lines. Understanding the lipidomic compositions and their associations with cell-cycle dynamics and proliferation allows for precise conditioning of culture media to promote sustainable growth and delay cellular entry into quiescence phases. This knowledge contributes to the long-term goal of maintaining robust prawn cell cultures for various research and biotechnological applications.
    Type of Medium: Online Resource
    ISSN: 2296-7745
    URL: Article
    URL: Article
    DOI: 10.3389/fmars.2023.1270491
    DOI: 10.3389/fmars.2023.1270491.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2757748-X
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  • 19
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    Identification of Receptor‐Interacting Regions of Vitellogenin within Evolutionarily Conserved β‐Sheet Structures by Using a Peptide Array
    Wiley ; 2013
    In:  ChemBioChem Vol. 14, No. 9 ( 2013-06-17), p. 1116-1122
    Details
    In: ChemBioChem, Wiley, Vol. 14, No. 9 ( 2013-06-17), p. 1116-1122
    Abstract: Vitellogenesis, a key process in oviparous animals, is characterized by enhanced synthesis of the lipoprotein vitellogenin, which serves as the major yolk‐protein precursor. In most oviparous animals, and specifically in crustaceans, vitellogenin is mainly synthesized in the hepatopancreas, secreted to the hemolymph, and taken up into the ovary by receptor‐mediated endocytosis. In the present study, localization of the vitellogenin receptor and its interaction with vitellogenin were investigated in the freshwater prawn Macrobrachium rosenbergii . The receptor was immuno‐histochemically localized to the cell periphery and around yolk vesicles. A receptor blot assay revealed that the vitellogenin receptor interacts with most known vitellogenin subunits, the most prominent being the 79 kDa subunit. The receptor was, moreover, able to interact with trypsin‐digested vitellogenin peptides. By combining a novel peptide‐array approach with tandem mass spectrometry, eleven vitellogenin‐derived peptides that interacted with the receptor were identified. A 3D model of vitellogenin indicated that four of the identified peptides are N‐terminally localized. One of the peptides is homologous to the receptor‐recognized site of vertebrate vitellogenin, and assumes a conserved β‐sheet structure. These findings suggest that this specific β‐sheet region in the vitellogenin N‐terminal lipoprotein domain is the receptor‐interacting site, with the rest of the protein serving to enhance affinity for the receptor. The conservation of the receptor recognition site in invertebrate and vertebrate vitellogenin might have vast implications for oviparous species reproduction, development, immunity, and pest management.
    Type of Medium: Online Resource
    ISSN: 1439-4227 , 1439-7633
    URL: Issue
    URL: Article
    DOI: 10.1002/cbic.v14.9
    DOI: 10.1002/cbic.201300152
    RVK:
    VA 2918
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2019276-9
    detail.hit.zdb_id: 2020469-3
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  • 20
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    Seasonal Plasma Levels of Luteinizing and Steroid Hormones in Male and Female Domestic Ostriches (Struthio camelus)
    Elsevier BV ; 1994
    In:  General and Comparative Endocrinology Vol. 93, No. 1 ( 1994-1), p. 21-27
    Details
    In: General and Comparative Endocrinology, Elsevier BV, Vol. 93, No. 1 ( 1994-1), p. 21-27
    Type of Medium: Online Resource
    ISSN: 0016-6480
    URL: Article
    DOI: 10.1006/gcen.1994.1003
    Language: English
    Publisher: Elsevier BV
    Publication Date: 1994
    detail.hit.zdb_id: 1851-X
    SSG: 12
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