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  • 1
    Online Resource
    Online Resource
    Elucidating the ecological networks in stone‐dwelling microbiomes
    Wiley ; 2020
    In:  Environmental Microbiology Vol. 22, No. 4 ( 2020-04), p. 1467-1480
    Details
    In: Environmental Microbiology, Wiley, Vol. 22, No. 4 ( 2020-04), p. 1467-1480
    Abstract: Stone surfaces are extreme environments that support microbial life. This microbial growth occurs despite unfavourable conditions associated with stone including limited sources of nutrients and water, high pH and exposure to extreme variations in temperature, humidity and irradiation. These stone‐dwelling microbes are often resistant to extreme environments including exposure to desiccation, heavy metals, UV and Gamma irradiation. Here, we report on the effects of climate and stone geochemistry on microbiomes of Roman stone ruins in North Africa. Stone microbiomes were dominated by Actinobacteria, Cyanobacteria and Proteobacteria but were heavily impacted by climate variables that influenced water availability. Stone geochemistry also influenced community diversity, particularly through biologically available P, Mn and Zn. Functions associated with photosynthesis and UV protection were enriched in the metagenomes, indicating the significance of these functions for community survival on stones. Core members of the stone microbial communities were also identified and included Geodermatophilaceae, Rubrobacter , Sphingomonas and others. Our research has helped to expand the understanding of stone microbial community structure and functional capacity within the context of varying climates, geochemical properties and stone conditions.
    Type of Medium: Online Resource
    ISSN: 1462-2912 , 1462-2920
    URL: Issue
    URL: Article
    DOI: 10.1111/emi.v22.4
    DOI: 10.1111/1462-2920.14700
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2020213-1
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Influence of Biological Treatment and Ultraviolet Disinfection System on Pseudomonas spp. Diversity in Wastewater as Assessed by Denaturing Gradient Gel Electrophoresis
    Wiley ; 2014
    In:  CLEAN – Soil, Air, Water Vol. 42, No. 5 ( 2014-05), p. 578-585
    Details
    In: CLEAN – Soil, Air, Water, Wiley, Vol. 42, No. 5 ( 2014-05), p. 578-585
    Abstract: Biological treatment methods use natural processes of ubiquitous living organisms to improve or upgrade the quality of a wastewater. To investigate the dominance of Pseudomonas spp. community in untreated and treated wastewater from full‐scale bio‐discs treatment plant, PCR–denaturing gradient gel electrophoresis coupled with sequence analysis of 16S rRNA gene fragments from dominant bands, were performed. The Pseudomonas species distribution over the total treatment process and over seasons (summer and winter) was obtained by comparing the denaturing gradient gel electrophoresis (DGGE) patterns to a normalized mix of nine reference Pseudomonas strains. Results indicated that Pseudomonas putida and Pseudomonas fluorescens strains showed dominance in wastewater, remarkable stability in the biofilm and resistance to UV irradiation. A reduction of Pseudomonas aeruginosa in treated wastewater was also observed. In addition, climatic and operational conditions results in selection of microbial community. The high temperature and intense solar radiation contribute to the total absence of the Pseudomonas syringae strain. In this work, the effect of increasing UV 254 germicidal dose on the Pseudomonas community in secondary treated wastewater effluent was also tested. At a dose of 1200 mWs/cm 2 , total disappearance of the band corresponding to P. aeruginosa , was noted. The DGGE approach can be used as an effective method to assess directly the Pseudomonas community shifts in studied wastewater treatment plant.
    Type of Medium: Online Resource
    ISSN: 1863-0650 , 1863-0669
    URL: Issue
    URL: Article
    DOI: 10.1002/clen.v42.5
    DOI: 10.1002/clen.201200589
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 2270984-8
    detail.hit.zdb_id: 2271097-8
    SSG: 13
    SSG: 14
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  • 3
    Online Resource
    Online Resource
    Purification and characterization of a highly thermostable esterase from the actinobacterium Geodermatophilus obscurus strain G20
    Wiley ; 2012
    In:  Journal of Basic Microbiology Vol. 52, No. 6 ( 2012-12), p. 653-660
    Details
    In: Journal of Basic Microbiology, Wiley, Vol. 52, No. 6 ( 2012-12), p. 653-660
    Abstract: Intracellular thermostable esterase produced by the extremophilic Geodermatophilus obscurus G20 was purified to homogeneity by a heat treatment, followed by an anion‐exchange chromatography, and then characterized. The molecular weight of the purified enzyme determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) was shown to be approximatively 55 kDa. The enzyme showed an optimal activity between pH 8.0 and 9.0 and was stable in the pH range 7.0–10.0. Moreover, it is highly thermostable, with a residual activity greater than 90% after incubation at 80 °C for more than 10 h. The enzyme showed preference for esters of p ‐nitrophenol with short chain fatty acid. When the p ‐nitrophenyl acetate (C2) was used as substrate, the Michaelis–Menten constant ( K m ) and maximum velocity for the reaction ( V max ) of esterase were 400 μM and 2500 U/mg protein, respectively. The effect of phenylmethanesulphonyl fluoride (PMSF), a serine‐specific inhibitor, on the enzyme activity suggested that the thermostable esterase belong to the serine hydrolase group. Because of its high thermostability, activity at alkaline pH, tolerance to methanol and various metal ions and specificity for short chain fatty acids, this enzyme showed high potential for use in biocatalysis. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
    Type of Medium: Online Resource
    ISSN: 0233-111X , 1521-4028
    URL: Issue
    URL: Article
    DOI: 10.1002/jobm.v52.6
    DOI: 10.1002/jobm.201100428
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 1480967-9
    detail.hit.zdb_id: 632513-0
    detail.hit.zdb_id: 203025-1
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Occurrence and diversity of Frankia in Tunisian soil
    Wiley ; 2007
    In:  Physiologia Plantarum Vol. 130, No. 3 ( 2007-07), p. 372-379
    Details
    In: Physiologia Plantarum, Wiley, Vol. 130, No. 3 ( 2007-07), p. 372-379
    Abstract: There is a lack of studies on the occurrence and diversity of Frankia in African soils, including those in northern African regions. The present study on Tunisian soils is an attempt to address this issue using Alnus glutinosa , Elaeagnus angustifolia and Casuarina glauca in a plant capturing bioassay on 30 soil samples, followed by amplified 16S ribosomal DNA restriction pattern analysis (ARDRA). A total of seven ARDRA haplotypes of Frankia have been detected in root actinorhizas that have been affiliated to theoretical ARDRA haplotypes upon in silico digestion of selected 16S ribosomal RNA (rRNA) gene sequences retrieved from GeneBank and confirmed by their partial 16S rRNA gene sequencing. Elaeagnus ‐compatible Frankia isolates were widespread and form four ARDRA haplotypes affiliated to Frankia , colonizing Elaeagnaceae and Rhamnaceae in two different phylogenetic subgroups. Alnus ‐compatible strains occurring in northern subhumid area were closely related to Alnus – Morella ‐compatible strains and clustered in two ARDRA haplotypes. Casuarina ‐compatible strains lack variability in several northern arboreta. The relatively wide diversity of Tunisian Frankia strains opens the perspective that African soil could be an interesting reservoir for the isolation of new actinorhizal strains that could be used as potential biofertilizers to counteract the progressive soil desertification which indeed is a crucial environmental problem in Northern Africa.
    Type of Medium: Online Resource
    ISSN: 0031-9317 , 1399-3054
    URL: Issue
    URL: Article
    DOI: 10.1111/ppl.2007.130.issue-3
    DOI: 10.1111/j.1399-3054.2006.00845.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2007
    detail.hit.zdb_id: 208872-1
    detail.hit.zdb_id: 2020837-6
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Biosynthetic energy cost of potentially highly expressed proteins vary with niche in selected actinobacteria
    Wiley ; 2018
    In:  Journal of Basic Microbiology Vol. 58, No. 2 ( 2018-02), p. 154-161
    Details
    In: Journal of Basic Microbiology, Wiley, Vol. 58, No. 2 ( 2018-02), p. 154-161
    Abstract: Amino acid and protein biosynthesis requires a number of high energy phosphate bonds and includes a dual energy cost for the synthesis of chemical intermediates during the fueling reactions and the conversion of precursor molecules to final products. One popular hypothesis is that the proteins encoded by putative highly expressed genes (hence called PHXPs) generally utilize low energy consuming amino acids to reduce the biosynthetic cost of the essential proteins. In our study, we found that this idea was not supported in the case of actinobacteria. With the actinobacteria, the energy costs of PHXPs varied in relation to their niche. Free‐living, including aquatic, soil and extremophilic, and plant‐associated actinobacteria were found to use energetically expensive amino acids in their PHXPs. An exception occurred with some animal‐host‐associated actinobacteria that used energy efficient amino acids. One explanation for these results may be due to the diverse metabolic patterns exhibited by actinobacteria under varied niches influenced by nutritional availability and physical environment.
    Type of Medium: Online Resource
    ISSN: 0233-111X , 1521-4028
    URL: Issue
    URL: Article
    DOI: 10.1002/jobm.v58.2
    DOI: 10.1002/jobm.201700350
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1480967-9
    detail.hit.zdb_id: 632513-0
    detail.hit.zdb_id: 203025-1
    SSG: 12
    Location Call Number Limitation Availability
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