In:
Biomedical Optics Express, Optica Publishing Group, Vol. 13, No. 11 ( 2022-11-01), p. 5574-
Kurzfassung:
Live-cell imaging reveals the phenotypes and mechanisms of cellular function and their dysfunction that underscore cell physiology, development, and pathology. Here, we report a 3D super-resolution live-cell microscopy method by integrating radiality analysis and Fourier light-field microscopy ( rad- FLFM). We demonstrated the method using various live-cell specimens, including actins in Hela cells, microtubules in mammary organoid cells, and peroxisomes in COS-7 cells. Compared with conventional wide-field microscopy, rad- FLFM realizes scanning-free, volumetric 3D live-cell imaging with sub-diffraction-limited resolution of ∼150 nm ( x-y ) and 300 nm ( z ), milliseconds volume acquisition time, six-fold extended depth of focus of ∼6 µm, and low photodamage. The method provides a promising avenue to explore spatiotemporal-challenging subcellular processes in a wide range of cell biological research.
Materialart:
Online-Ressource
ISSN:
2156-7085
,
2156-7085
Sprache:
Englisch
Verlag:
Optica Publishing Group
Publikationsdatum:
2022
ZDB Id:
2572216-5
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