In:
Parasitology, Cambridge University Press (CUP), Vol. 100, No. 1 ( 1990-02), p. 73-81
Abstract:
The relationship between antigens associated with the surface of newly transformed schistosomula of Schistosoma mansoni and the tegumental surface membrane of adult S. mansoni worms has been further explored. Immunoprecipitation of detergent-solubilized 125 I-tegumental surface membrane antigens of adult S. mansoni with antibodies from mice vaccinated with highly irradiated S. mansoni cercariae revealed major antigens of M r 32, 20, 15 and 8K. The M r 32 and 20K antigens have been previously demonstrated to be antigenically and electrophoretically identical to major antigens on the schistosomulum surface. The M r 15 and 8K antigens, on the other hand, have not been identified by the immunoprecipitation of 125 I-schistosomulum surface antigens, although a distinct schistosomulum surface antigen of M r 15K is precipitated by antibodies from mice vaccinated with highly irradiated cercariae. Nevertheless, it was shown that antibodies to the M r 15 and 8K antigens were specifically absorbed from vaccinated mouse serum by intact, live schistosomula, demonstrating that the M r 15 and 8K antigens are exposed on or released from the schistosomulum surface. In contrast, absorption of the antiserum with eggs failed to remove antibody against any of the four tegumental membrane antigens examined. The M r 15 and 8K antigens were shown to be recognized via polypeptide epitopes and not periodate-sensitive carbohydrate epitopes, further emphasizing the similarity of these to the well-characterized M r 32 and 20K tegumental surface membrane antigens. A general relationship between schistosomulum surface, adult tegumental membrane and egg antigens was demonstrated by ELISA, using antibodies raised against the three antigenic fractions. It was shown that both the egg and adult tegumental membrane antigens cross-react with the schistosomulum surface, but that the egg and adult membrane antigens exhibit very little, if any, mutual cross-reactivity. This antigen divergence possibly enables the host to dissociate pathological, anti-egg responses from potentially protective anti-membrane responses during the course of natural infection. It also suggests that adult membrane antigens could be used in an anti-schistosome vaccine without the possible complication of inducing pathological responses.
Type of Medium:
Online Resource
ISSN:
0031-1820
,
1469-8161
DOI:
10.1017/S0031182000060133
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
1990
detail.hit.zdb_id:
1491287-9
SSG:
12
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